The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
02 10 2023
02 10 2023
Historique:
received:
28
01
2023
revised:
31
05
2023
accepted:
06
07
2023
medline:
31
7
2023
pubmed:
30
7
2023
entrez:
30
7
2023
Statut:
ppublish
Résumé
The endoplasmic reticulum's (ER's) structure is directly linked to the many functions of the ER, but its formation is not fully understood. We investigate how the ER-membrane curving protein reticulon 4 (Rtn4) localizes to and organizes in the membrane and how that affects the local ER structure. We show a strong correlation between the local Rtn4 density and the local ER membrane curvature. Our data further reveal that the typical ER tubule possesses an elliptical cross-section with Rtn4 enriched at either end of the major axis. Rtn4 oligomers are linear shaped, contain about five copies of the protein, and preferentially orient parallel to the tubule axis. Our observations support a mechanism in which oligomerization leads to an increase of the local Rtn4 concentration with each molecule, increasing membrane curvature through a hairpin wedging mechanism. This quantitative analysis of Rtn4 and its effects on the ER membrane result in a new model of tubule shape as it relates to Rtn4.
Identifiants
pubmed: 37516910
pii: 276129
doi: 10.1083/jcb.202301112
pmc: PMC10373298
pii:
doi:
Substances chimiques
Nogo Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Wellcome Trust
ID : 203285/B/16/Z
Pays : United Kingdom
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023 Fuentes et al.
Références
Science. 2016 Oct 28;354(6311):
pubmed: 27789813
Mol Biol Cell. 2012 Jul;23(13):2424-32
pubmed: 22573885
Nat Methods. 2020 Mar;17(3):261-272
pubmed: 32015543
EMBO J. 2005 Dec 21;24(24):4368-80
pubmed: 16362047
Nat Commun. 2016 Mar 04;7:10778
pubmed: 26940217
Science. 2008 Feb 29;319(5867):1247-50
pubmed: 18309084
Science. 2011 Oct 21;334(6054):358-62
pubmed: 21885730
J Cell Biol. 2019 Jan 7;218(1):83-96
pubmed: 30442642
Nat Methods. 2020 Feb;17(2):225-231
pubmed: 31907447
Cell. 2006 Feb 10;124(3):573-86
pubmed: 16469703
Annu Rev Biochem. 2018 Jun 20;87:965-989
pubmed: 29272143
Trends Cell Biol. 2003 Apr;13(4):187-94
pubmed: 12667756
Biophys J. 2023 Jun 23;:
pubmed: 37355772
Curr Opin Cell Biol. 2013 Aug;25(4):428-33
pubmed: 23478217
Sci Rep. 2017 Nov 28;7(1):16474
pubmed: 29184084
Cell. 2013 Jul 18;154(2):285-96
pubmed: 23870120
Traffic. 2011 Jan;12(1):28-41
pubmed: 20955502
Dev Cell. 2022 Jun 6;57(11):1369-1382.e6
pubmed: 35609616
Dev Cell. 2021 Dec 20;56(24):3364-3379.e10
pubmed: 34852214
Genome Biol. 2007;8(12):234
pubmed: 18177508
J Cell Biol. 2020 Apr 6;219(4):
pubmed: 32328629
Nat Commun. 2021 Jan 25;12(1):568
pubmed: 33495454
Nature. 2022 Jan;601(7891):132-138
pubmed: 34912111
Nat Methods. 2019 Oct;16(10):1045-1053
pubmed: 31562488
Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):E5243-51
pubmed: 25404289
Nat Methods. 2021 Jun;18(6):582-584
pubmed: 34002092
Cell. 2010 Nov 24;143(5):774-88
pubmed: 21111237
Philos Trans A Math Phys Eng Sci. 2016 Apr 13;374(2065):20150202
pubmed: 26953178
Nat Methods. 2022 May;19(5):554-559
pubmed: 35501386
ACS Cent Sci. 2021 Aug 25;7(8):1419-1426
pubmed: 34471685
J Struct Biol. 2007 Nov;160(2):224-35
pubmed: 17889556
Biol Reprod. 2018 Jul 1;99(1):101-111
pubmed: 29566165
Nature. 2022 Mar;603(7902):736-742
pubmed: 35264794
Science. 2014 Nov 7;346(6210):1257521
pubmed: 25378630
Biophys J. 2018 Sep 18;115(6):951-956
pubmed: 30139523
J Cell Sci. 2019 Jan 22;132(4):
pubmed: 30670475
Cell. 2018 Sep 20;175(1):254-265.e14
pubmed: 30220460
Cold Spring Harb Perspect Biol. 2023 Jun 1;15(6):
pubmed: 36123032
Int J Biochem Cell Biol. 2012 Sep;44(9):1436-43
pubmed: 22640864
Biochim Biophys Acta. 2013 Nov;1833(11):2492-8
pubmed: 23602970
Annu Rev Cell Dev Biol. 2009;25:113-32
pubmed: 19575639
J Cell Biol. 2011 Apr 18;193(2):333-46
pubmed: 21502358
Nat Protoc. 2018 Jun;13(6):1465-1487
pubmed: 29844520
Proc Natl Acad Sci U S A. 2022 May 3;119(18):e2117559119
pubmed: 35471903
Nat Methods. 2020 Sep;17(9):870-872
pubmed: 32814874
J Biol Chem. 2008 Jul 4;283(27):18892-904
pubmed: 18442980
Cell. 2016 Aug 11;166(4):1028-1040
pubmed: 27397506