Optimizing conditions to construct artificial cells using commercial in vitro transcription-translation system (PUREfrex2.0).

Liposomes Artificial Cells
Artificial cells Constructive biology In vitro transcription-translation Liposomes PUREfrex2.0

Journal

Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800

Informations de publication

Date de publication:
Oct 2023
Historique:
received: 08 05 2023
revised: 03 07 2023
accepted: 12 07 2023
medline: 15 9 2023
pubmed: 31 7 2023
entrez: 30 7 2023
Statut: ppublish

Résumé

Artificial cells containing in vitro transcription and translation (IVTT) systems inside liposomes are important for the reconstruction and analysis of various biological systems. To improve the accessibility of artificial cell research, it is important that artificial cells can be constructed using only commercially available components. Here, we optimized the construction of artificial cells containing PUREfrex2.0, a commercially available IVTT with high transcriptional and translational activity. Specifically, the composition of the inner and outer s olutions of the liposomes and the concentrations of lipids, glucose/sucrose, potassium glutamate, and magnesium acetate were systematically optimized, and finally we found a protocol for the stable construction of artificial cells containing PUREfre×2.0. These findings are expected to be important in expanding the artificial cell research community.

Identifiants

DOI: 10.1016/j.jbiosc.2023.07.004 PMID: 37517904
pubmed: 37517904
pii: S1389-1723(23)00231-1
doi: 10.1016/j.jbiosc.2023.07.004
pii:
doi:

Substances chimiques

Liposomes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

334-339

Informations de copyright

Copyright © 2023 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Auteurs

Toshiko Sato (T)

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. Electronic address: sato.toshiko.7v@kyoto-u.ac.jp.

Shuhei Matsuda (S)

World Intec Co., Ltd., Fukuoka 812-0011, Japan. Electronic address: s.matsuda.y35@rd.witc.co.jp.

Wataru Aoki (W)

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. Electronic address: aoki.wataru@bio.eng.osaka-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Liposomes › Optimizing conditions to construct artificial...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Cellules artificielles › Optimizing conditions to construct artificial...