Long wavelength-sensing cones of zebrafish retina exhibit multiple layers of transcriptional heterogeneity.
RNA-Seq
cone
opsin
photoreceptor
retina
scRNA-Seq
transducin
zebrafish
Journal
Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935
Informations de publication
Date de publication:
2023
2023
Historique:
received:
28
04
2023
accepted:
26
06
2023
medline:
31
7
2023
pubmed:
31
7
2023
entrez:
31
7
2023
Statut:
epublish
Résumé
Understanding how photoreceptor genes are regulated is important for investigating retinal development and disease. While much is known about gene regulation in cones, the mechanism by which tandemly-replicated opsins, such as human long wavelength-sensitive and middle wavelength-sensitive opsins, are differentially regulated remains elusive. In this study, we aimed to further our understanding of transcriptional heterogeneity in cones that express tandemly-replicated opsins and the regulation of such differential expression using zebrafish, which express the tandemly-replicated opsins We performed bulk and single cell RNA-Seq of LWS1 and LWS2 cones, evaluated expression patterns of selected genes of interest using multiplex fluorescence Our studies indicate that additional transcriptional differences beyond opsin expression exist between LWS1 and LWS2 cones. Bulk RNA-Seq results showed 95 transcripts enriched in LWS1 cones and 186 transcripts enriched in LWS2 cones (FC > 2, FDR < 0.05). We found some evidence of coordinate regulation of
Identifiants
pubmed: 37519633
doi: 10.3389/fncel.2023.1214084
pmc: PMC10382231
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1214084Subventions
Organisme : NEI NIH HHS
ID : F31 EY031962
Pays : United States
Organisme : NEI NIH HHS
ID : F31 EY034793
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM103408
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY012146
Pays : United States
Informations de copyright
Copyright © 2023 Farre, Sun, Starostik, Hunter, English, Duncan, Santhanam, Shihabeddin, O’Brien, Swaroop and Stenkamp.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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