Early protective effect of a ("pan") coronavirus vaccine (PanCoVac) in Roborovski dwarf hamsters after single-low dose intranasal administration.
T cell epitopes
T-cell-directed vaccine
coronaviruses
dwarf hamster COVID-19 model
multi-epitope vaccine
pan-coronavirus vaccine
universal COVID-19 vaccine
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2023
2023
Historique:
received:
15
02
2023
accepted:
19
06
2023
medline:
1
8
2023
pubmed:
31
7
2023
entrez:
31
7
2023
Statut:
epublish
Résumé
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted the danger posed by human coronaviruses. Rapid emergence of immunoevasive variants and waning antiviral immunity decrease the effect of the currently available vaccines, which aim at induction of neutralizing antibodies. In contrast, T cells are marginally affected by antigen evolution although they represent the major mediators of virus control and vaccine protection against virus-induced disease. We generated a multi-epitope vaccine (PanCoVac) that encodes the conserved T cell epitopes from all structural proteins of coronaviruses. PanCoVac contains elements that facilitate efficient processing and presentation of PanCoVac-encoded T cell epitopes and can be uploaded to any available vaccine platform. For proof of principle, we cloned PanCoVac into a non-integrating lentivirus vector (NILV-PanCoVac). We chose Roborovski dwarf hamsters for a first step in evaluating PanCoVac Using HLA-A PanCoVac, a multi-epitope vaccine covering conserved T cell epitopes from all structural proteins of coronaviruses, might protect from severe disease caused by SARS-CoV-2 variants and future pathogenic coronaviruses. The use of (HLA-) humanized animal models will allow for further efficacy studies of PanCoVac-based vaccines
Identifiants
pubmed: 37520530
doi: 10.3389/fimmu.2023.1166765
pmc: PMC10372429
doi:
Substances chimiques
COVID-19 Vaccines
0
Epitopes, T-Lymphocyte
0
Viral Vaccines
0
Antibodies, Neutralizing
0
HLA-A Antigens
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1166765Informations de copyright
Copyright © 2023 Abdelaziz, Raftery, Weihs, Bielawski, Edel, Köppke, Vladimirova, Adler, Firsching, Voß, Gruber, Hummel, Fernandez Munoz, Müller-Marquardt, Willimsky, Elleboudy, Trimpert and Schönrich.
Déclaration de conflit d'intérêts
MA, MR and GS hold a patent EP4176898 related to the PanCoVac vaccine described herein. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
N Engl J Med. 2021 Dec 9;385(24):e83
pubmed: 34614327
Immunity. 2022 May 10;55(5):738-748
pubmed: 35545026
Hum Vaccin Immunother. 2022 Nov 30;18(5):2045853
pubmed: 35258416
N Engl J Med. 2021 Dec 9;385(24):e85
pubmed: 34706170
Nature. 2022 Jan;601(7891):110-117
pubmed: 34758478
Nat Rev Microbiol. 2022 May;20(5):299-314
pubmed: 34799704
J Infect. 2020 Dec;81(6):923-930
pubmed: 33127456
Nature. 2020 Aug;584(7821):457-462
pubmed: 32668444
JAMA Netw Open. 2022 Apr 1;5(4):e2210871
pubmed: 35452102
Cell Rep Med. 2022 Feb 25;3(3):100562
pubmed: 35474748
J Virol. 2013 Jul;87(14):7790-2
pubmed: 23678167
J Immunol. 2011 Jun 15;186(12):7264-8
pubmed: 21576510
Proc Natl Acad Sci U S A. 2023 Mar 21;120(12):e2220320120
pubmed: 36917669
Science. 2021 Oct 08;374(6564):eabh1823
pubmed: 34465633
J Clin Invest. 2021 Jan 4;131(1):
pubmed: 32997649
Sci Immunol. 2022 May 20;7(71):eabo1303
pubmed: 35324269
Sci Rep. 2020 Dec 8;10(1):21447
pubmed: 33293664
Cell Rep. 2020 Dec 8;33(10):108488
pubmed: 33271063
Vaccine. 2016 Apr 12;34(17):2008-14
pubmed: 26954467
Commun Biol. 2021 Jun 10;4(1):713
pubmed: 34112936
Cell Rep Med. 2022 Mar 04;3(3):100557
pubmed: 35474751
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
Nat Rev Microbiol. 2021 Jul;19(7):409-424
pubmed: 34075212
Cell. 2020 Oct 1;183(1):158-168.e14
pubmed: 32979941
Proc Natl Acad Sci U S A. 2022 May 17;119(20):e2118312119
pubmed: 35561224
Immunity. 2022 Dec 13;55(12):2231-2235
pubmed: 36309008
Nat Commun. 2019 Dec 4;10(1):5531
pubmed: 31797935
Science. 2022 Nov 25;378(6622):eabo2523
pubmed: 36302057
Sci Immunol. 2022 Oct 28;7(76):eadd4853
pubmed: 35857583
Immunol Rev. 2022 Sep;310(1):27-46
pubmed: 35733376
Pediatr Allergy Immunol. 2020 Jul;31(5):565-569
pubmed: 32319118
Cell. 2020 Jun 25;181(7):1489-1501.e15
pubmed: 32473127
Nat Rev Immunol. 2011 Nov 11;11(12):823-36
pubmed: 22076556
Sci Transl Med. 2021 Apr 21;13(590):
pubmed: 33723016
Mult Scler Relat Disord. 2021 Oct;55:103157
pubmed: 34418737
Biomedicines. 2014 Jan 27;2(1):14-35
pubmed: 28548058
Front Immunol. 2019 Jul 30;10:1776
pubmed: 31417555
Nat Commun. 2022 Jul 8;13(1):3966
pubmed: 35803932
N Engl J Med. 2003 May 15;348(20):1986-94
pubmed: 12682352
Front Immunol. 2023 Jan 27;14:1107803
pubmed: 36776863
Arch Virol. 2009;154(7):1093-9
pubmed: 19526193
Nat Immunol. 2021 Jan;22(1):74-85
pubmed: 32999467
NPJ Vaccines. 2022 Dec 19;7(1):167
pubmed: 36535982
Cell Rep Med. 2021 Jul 20;2(7):100355
pubmed: 34230917
J Virol. 1998 Nov;72(11):8463-71
pubmed: 9765382
Lancet. 1961 Sep 30;2(7205):748-9
pubmed: 14474038
Nat Commun. 2021 Aug 17;12(1):5061
pubmed: 34404775
Nat Rev Immunol. 2023 May;23(5):304-316
pubmed: 36539527
BMC Biotechnol. 2006 Jul 12;6:34
pubmed: 16836756
Sci Adv. 2021 Dec 03;7(49):eabk0172
pubmed: 34851677
Cell. 2022 Feb 3;185(3):467-484.e15
pubmed: 35081335
J Virol. 2011 Oct;85(20):10464-71
pubmed: 21813600
Immunity. 2022 May 10;55(5):749-780
pubmed: 35545027
Nat Immunol. 2022 Feb;23(2):186-193
pubmed: 35105982
Immunol Rev. 1997 Oct;159:105-17
pubmed: 9416506
J Immunol. 2022 Mar 1;208(5):1001-1005
pubmed: 35121642
Nat Commun. 2021 Nov 26;12(1):6871
pubmed: 34836955
Nat Immunol. 2022 Sep;23(9):1324-1329
pubmed: 36038709
Hum Vaccin Immunother. 2022 Nov 30;18(6):2127292
pubmed: 36194255
Sci Immunol. 2020 Jun 26;5(48):
pubmed: 32591408
Immunity. 2021 May 11;54(5):1055-1065.e5
pubmed: 33945786
Nat Rev Immunol. 2021 Nov;21(11):693
pubmed: 34611319
Cell. 2021 Jan 7;184(1):169-183.e17
pubmed: 33296701
Sci Immunol. 2022 Mar 25;7(69):eabo2202
pubmed: 35113647
Immunity. 2022 Oct 11;55(10):1764-1778
pubmed: 36049482
Immunity. 2016 Jun 21;44(6):1379-91
pubmed: 27287409
Nucleic Acids Res. 2022 Jul 5;50(W1):W36-W43
pubmed: 35640594
Nucleic Acids Res. 2020 Jul 2;48(W1):W449-W454
pubmed: 32406916
Cell Rep. 2022 Jun 14;39(11):110952
pubmed: 35675811
Lancet Infect Dis. 2022 Sep;22(9):1293-1302
pubmed: 35753318
Pediatr Res. 2004 Jul;56(1):1-5
pubmed: 15152053
Nat Commun. 2022 Aug 9;13(1):4380
pubmed: 35945197
Vaccine. 2010 Sep 24;28(41):6675-83
pubmed: 20709004
Signal Transduct Target Ther. 2022 May 3;7(1):146
pubmed: 35504917
Nat Commun. 2021 Apr 6;12(1):2055
pubmed: 33824342
Emerg Infect Dis. 2021 Jan;27(1):
pubmed: 33261718
Science. 2021 Apr 16;372(6539):227-231
pubmed: 33859017
Nat Immunol. 2021 May;22(5):620-626
pubmed: 33674800
EBioMedicine. 2022 Feb;76:103841
pubmed: 35085851
BMC Bioinformatics. 2007 Jan 05;8:4
pubmed: 17207271
Annu Rev Immunol. 2006;24:519-40
pubmed: 16551258
Sci Immunol. 2022 Dec 23;7(78):eadd3899
pubmed: 36318037
Science. 2021 Oct 22;374(6566):eabj9853
pubmed: 34519540
J Exp Med. 1995 Dec 1;182(6):1865-70
pubmed: 7500032
J Immunol. 2021 Jul 15;207(2):376-379
pubmed: 34193597
Nat Med. 2021 Jan;27(1):78-85
pubmed: 33184509
Cell. 1991 Sep 20;66(6):1145-53
pubmed: 1913805
N Engl J Med. 2022 Jan 27;386(4):297-299
pubmed: 34910863
J Immunol. 1995 Jun 1;154(11):5934-43
pubmed: 7538538
Nat Commun. 2022 Jan 10;13(1):80
pubmed: 35013199
J Virol. 2014 Oct;88(19):11034-44
pubmed: 25056892
Nature. 2021 Feb;590(7847):630-634
pubmed: 33276369
NPJ Vaccines. 2020 May 28;5(1):43
pubmed: 32528732
Nature. 2021 Sep;597(7878):603
pubmed: 34561634
Cell. 2022 Mar 3;185(5):847-859.e11
pubmed: 35139340
Lancet Microbe. 2022 May;3(5):e348-e356
pubmed: 35345417
Mol Ther. 2008 Apr;16(4):698-706
pubmed: 18362927
N Engl J Med. 2017 Feb 9;376(6):584-594
pubmed: 28177862
Proc (Bayl Univ Med Cent). 2008 Jan;21(1):3-8
pubmed: 18209746
N Engl J Med. 2022 Feb 10;386(6):599-601
pubmed: 35030645
Vaccines (Basel). 2021 Aug 09;9(8):
pubmed: 34452006
Nature. 2022 Jan;601(7894):617-622
pubmed: 34814158
Nature. 2020 Nov;587(7833):270-274
pubmed: 32726801
Nature. 2022 Apr;604(7905):330-336
pubmed: 35172323
Science. 2020 Oct 2;370(6512):89-94
pubmed: 32753554
JAMA. 2022 Apr 19;327(15):1438
pubmed: 35438724
PLoS Pathog. 2022 Feb 17;18(2):e1010260
pubmed: 35176090
Sci Immunol. 2021 Oct 22;6(64):eabk0894
pubmed: 34519539
Trends Mol Med. 2023 Apr;29(4):255-267
pubmed: 36764906
J Exp Med. 2021 Apr 5;218(4):
pubmed: 33464307
PLoS One. 2013 Sep 13;8(9):e73957
pubmed: 24058508
Nat Rev Mol Cell Biol. 2002 Oct;3(10):753-66
pubmed: 12360192
Science. 2022 Jan 21;375(6578):331-336
pubmed: 34735261
Sci Immunol. 2021 May 25;6(59):
pubmed: 34035118
Nature. 2022 Mar;603(7901):488-492
pubmed: 35102311
J Clin Invest. 2021 Jan 4;131(1):
pubmed: 33216734
Cell. 2021 Aug 19;184(17):4380-4391.e14
pubmed: 34147139
Cell. 2022 Jul 7;185(14):2434-2451.e17
pubmed: 35764089
Nat Med. 2023 Feb;29(2):334-343
pubmed: 36797482
Nature. 2022 Mar;603(7901):493-496
pubmed: 35102312
Cell Host Microbe. 2021 Feb 10;29(2):236-249.e6
pubmed: 33357418
Lancet Reg Health Eur. 2021 Nov;10:100208
pubmed: 34514454
Annu Rev Immunol. 2023 Apr 26;41:343-373
pubmed: 36750314
Nat Microbiol. 2020 Apr;5(4):536-544
pubmed: 32123347
J Med Virol. 2022 Jun;94(6):2402-2413
pubmed: 35099819
Oncotarget. 2017 May 4;8(39):64892-64906
pubmed: 29029399
J Gene Med. 2006 Jan;8(1):3-17
pubmed: 16288497
Infect Genet Evol. 2020 Nov;85:104502
pubmed: 32798769
Hum Gene Ther. 2002 Jun 10;13(9):1091-100
pubmed: 12067442
Nat Rev Immunol. 2020 Aug;20(8):457-458
pubmed: 32636479
Mult Scler. 2020 Sep;26(10):1261-1264
pubmed: 32762494
Nat Med. 2015 Dec;21(12):1508-13
pubmed: 26552008
Science. 2022 Mar 11;375(6585):1127-1132
pubmed: 35271316
mBio. 2022 Apr 26;13(2):e0361721
pubmed: 35229637
Traffic. 2000 Aug;1(8):641-51
pubmed: 11208152
Cell Rep Med. 2022 Jan 19;3(2):100520
pubmed: 35233545
Methods Mol Biol. 2004;246:451-9
pubmed: 14970610
Viruses. 2021 Nov 16;13(11):
pubmed: 34835096
JAMA Neurol. 2022 Apr 01;79(4):399-404
pubmed: 35212717
Front Immunol. 2015 Jun 05;6:298
pubmed: 26097483
Med. 2023 Mar 10;4(3):191-215.e9
pubmed: 36863347
J Immunol. 2021 Jun 1;206(11):2566-2582
pubmed: 33911008
Cell Rep. 2021 Jul 20;36(3):109400
pubmed: 34245672
Mol Immunol. 2015 Dec;68(2 Pt A):72-6
pubmed: 26006050
Nat Rev Microbiol. 2023 Mar;21(3):162-177
pubmed: 36653446
Sci Immunol. 2021 Jul 1;6(61):
pubmed: 34210785
Cell Rep Med. 2021 Feb 16;2(2):100204
pubmed: 33521695
Nat Commun. 2021 Nov 9;12(1):6563
pubmed: 34753934
BMC Immunol. 2008 Jan 22;9:1
pubmed: 18211710
Viruses. 2020 Jul 20;12(7):
pubmed: 32698441
Front Immunol. 2021 Dec 17;12:781280
pubmed: 34987509
Cell Host Microbe. 2021 Jul 14;29(7):1076-1092
pubmed: 34237248
PLoS Comput Biol. 2022 Feb 10;18(2):e1009726
pubmed: 35143484
Nat Med. 2022 Mar;28(3):472-476
pubmed: 35042228
Mol Ther. 2007 Feb;15(2):411-21
pubmed: 17235321
Cell. 2022 Mar 3;185(5):896-915.e19
pubmed: 35180381