The bioinformatics analysis of CD59 in Helicobacter pylori infected gastric cancer.


Journal

Cancer biomarkers : section A of Disease markers
ISSN: 1875-8592
Titre abrégé: Cancer Biomark
Pays: Netherlands
ID NLM: 101256509

Informations de publication

Date de publication:
2023
Historique:
medline: 12 9 2023
pubmed: 31 7 2023
entrez: 31 7 2023
Statut: ppublish

Résumé

Cell surface molecules play important roles in cell signal transduction pathways during microbial infection. In this study, the expression and the functions of CD59 was investigated in H. pylori infected gastric cancer (GC). The differential expression of CD59 and the influence of H. pylori on the expression of CD59 were analyzed via bioinformatics through Gene Set Enrichment in GC. In addition, the expression of CD59 in GES-1, AGS cells and GC tissues infected with H. pylori was confirmed by Western blot. Bioinformatics results and H. pylori infection experiments showed CD59 decreased obviously in H. pylori infected GC cells and tissues. The expression of CD59 was linked to the survival rate of GC patients, and influenced various immune cells in the immune microenvironment of GC. CD59 interacts with other genes to form a network in H. pylori infected GC. Certainly, CD59 decreased significantly in H. pylori infected GC tissues, GES-1 and AGS cells in vitro. H. pylori infection could influence the expression of CD59 in GC indicating that CD59 may be a promising treatment target.

Sections du résumé

BACKGROUND BACKGROUND
Cell surface molecules play important roles in cell signal transduction pathways during microbial infection.
OBJECTIVE OBJECTIVE
In this study, the expression and the functions of CD59 was investigated in H. pylori infected gastric cancer (GC).
METHODS AND RESULTS RESULTS
The differential expression of CD59 and the influence of H. pylori on the expression of CD59 were analyzed via bioinformatics through Gene Set Enrichment in GC. In addition, the expression of CD59 in GES-1, AGS cells and GC tissues infected with H. pylori was confirmed by Western blot. Bioinformatics results and H. pylori infection experiments showed CD59 decreased obviously in H. pylori infected GC cells and tissues. The expression of CD59 was linked to the survival rate of GC patients, and influenced various immune cells in the immune microenvironment of GC. CD59 interacts with other genes to form a network in H. pylori infected GC. Certainly, CD59 decreased significantly in H. pylori infected GC tissues, GES-1 and AGS cells in vitro.
CONCLUSION CONCLUSIONS
H. pylori infection could influence the expression of CD59 in GC indicating that CD59 may be a promising treatment target.

Identifiants

pubmed: 37522198
pii: CBM230034
doi: 10.3233/CBM-230034
doi:

Substances chimiques

CD59 protein, human 101754-01-2
CD59 Antigens 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

27-35

Auteurs

Jun Sun (J)

Department of Medical Service, Kunshan First People's Hospital, Kunshan, Jiangsu, China.
Department of Medical Service, Kunshan First People's Hospital, Kunshan, Jiangsu, China.

Hui Cao (H)

Department of food safety and evaluation, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu, China.
Department of Medical Service, Kunshan First People's Hospital, Kunshan, Jiangsu, China.

Tingting Wen (T)

Department of Pharmacy, Kunshan First People's Hospital, Kunshan, Jiangsu, China.

Zi Xu (Z)

Department of Clinical Laboratory, Kunshan First People's Hospital, Kunshan, Jiangsu, China.

Xian Zhang (X)

Department of Clinical Laboratory, Kunshan First People's Hospital, Kunshan, Jiangsu, China.

Jianjun Wang (J)

Department of Clinical Laboratory, Kunshan First People's Hospital, Kunshan, Jiangsu, China.

Hong Zhu (H)

Department of Clinical Laboratory, the Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu, China.

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Classifications MeSH