Novel anti-PTEN C2 domain monoclonal antibodies to analyse the expression and function of PTEN isoform variants.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
11
04
2023
accepted:
17
07
2023
medline:
3
8
2023
pubmed:
1
8
2023
entrez:
1
8
2023
Statut:
epublish
Résumé
PTEN is a major tumor suppressor gene frequently mutated in human tumors, and germline PTEN gene mutations are the molecular diagnostic of PTEN Hamartoma Tumor Syndrome (PHTS), a heterogeneous disorder that manifests with multiple hamartomas, cancer predisposition, and neurodevelopmental alterations. A diversity of translational and splicing PTEN isoforms exist, as well as PTEN C-terminal truncated variants generated by disease-associated nonsense mutations. However, most of the available anti-PTEN monoclonal antibodies (mAb) recognize epitopes at the PTEN C-terminal tail, which may introduce a bias in the analysis of the expression of PTEN isoforms and variants. We here describe the generation and precise characterization of anti-PTEN mAb recognizing the PTEN C2-domain, and their use to monitor the expression and function of PTEN isoforms and PTEN missense and nonsense mutations associated to disease. These anti-PTEN C2 domain mAb are suitable to study the pathogenicity of PTEN C-terminal truncations that retain stability and function but have lost the PTEN C-terminal epitopes. The use of well-defined anti-PTEN mAb recognizing distinct PTEN regions, as the ones here described, will help to understand the deleterious effects of specific PTEN mutations in human disease.
Identifiants
pubmed: 37527256
doi: 10.1371/journal.pone.0289369
pii: PONE-D-23-09497
pmc: PMC10393154
doi:
Substances chimiques
Codon, Nonsense
0
PTEN Phosphohydrolase
EC 3.1.3.67
Protein Isoforms
0
Epitopes
0
Antibodies, Monoclonal
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0289369Informations de copyright
Copyright: © 2023 Torices et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Int Immunopharmacol. 2020 Aug;85:106639
pubmed: 32473573
J Biomed Sci. 2020 Jan 2;27(1):1
pubmed: 31894001
EMBO J. 2021 May 17;40(10):e105806
pubmed: 33755220
Cell Mol Life Sci. 2017 Aug;74(15):2783-2794
pubmed: 28289760
J Invest Dermatol. 2003 Dec;121(6):1356-9
pubmed: 14675182
Eur J Hum Genet. 2023 May;31(5):568-577
pubmed: 36543932
Science. 2013 Jul 26;341(6144):399-402
pubmed: 23744781
Hum Mol Genet. 2006 Mar 1;15(5):777-87
pubmed: 16436456
Semin Cell Dev Biol. 2016 Apr;52:30-8
pubmed: 26827793
Nat Commun. 2021 Aug 26;12(1):5147
pubmed: 34446716
Mol Biosyst. 2013 Nov;9(11):2877-88
pubmed: 24056727
PLoS One. 2016 Aug 22;11(8):e0160972
pubmed: 27548698
Sci Signal. 2014 Jul 01;7(332):pe15
pubmed: 24985344
Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10182-7
pubmed: 10468583
Methods. 2015 May;77-78:3-10
pubmed: 25843297
J Cell Biochem. 2012 Aug;113(8):2661-70
pubmed: 22434720
Hum Mol Genet. 2005 Aug 15;14(16):2459-68
pubmed: 16014636
Nat Cell Biol. 2019 Nov;21(11):1436-1448
pubmed: 31685992
Cancer Lett. 2005 Jun 8;223(2):303-12
pubmed: 15896465
Pharmacol Res Perspect. 2019 Dec;7(6):e00535
pubmed: 31859459
Fac Rev. 2020 Dec 23;9:31
pubmed: 33659963
Methods Mol Biol. 2016;1388:169-86
pubmed: 27033077
Eur J Hum Genet. 2018 Aug;26(8):1180-1187
pubmed: 29706633
Open Biol. 2016 May;6(5):
pubmed: 27249819
Sci Rep. 2019 Feb 28;9(1):3183
pubmed: 30816308
Cell. 1999 Oct 29;99(3):323-34
pubmed: 10555148
Am J Pathol. 1999 Oct;155(4):1253-60
pubmed: 10514407
Cancers (Basel). 2019 Jun 18;11(6):
pubmed: 31216739
Eur J Cancer. 2001 Jan;37(1):114-21
pubmed: 11165138
Cancer Res. 2007 Oct 15;67(20):9731-9
pubmed: 17942903
Hum Mutat. 2021 May;42(5):551-566
pubmed: 33600059
Blood. 2019 May 9;133(19):2079-2089
pubmed: 30926592
PLoS One. 2015 Jan 21;10(1):e0116898
pubmed: 25607987
Biochim Biophys Acta. 2000 Dec 1;1494(3):282-5
pubmed: 11121587
Mol Biomed. 2022 Nov 22;3(1):35
pubmed: 36418786
Cells. 2019 Nov 26;8(12):
pubmed: 31779149
Cell Commun Signal. 2018 Jun 28;16(1):35
pubmed: 29954386
Annu Rev Med. 2020 Jan 27;71:103-116
pubmed: 31433956
Cold Spring Harb Perspect Med. 2019 Nov 1;9(11):
pubmed: 31451538
NPJ Precis Oncol. 2019 Apr 15;3:11
pubmed: 30993208
Cold Spring Harb Perspect Med. 2019 Dec 2;9(12):
pubmed: 31501265
Nat Rev Mol Cell Biol. 2018 Sep;19(9):547-562
pubmed: 29858604
Annu Rev Biochem. 2014;83:641-69
pubmed: 24905788
J Biol Chem. 2003 Aug 15;278(33):30652-60
pubmed: 12788938
Cell Metab. 2014 May 6;19(5):836-48
pubmed: 24768297
Biomolecules. 2023 Jan 30;13(2):
pubmed: 36830628
Nat Commun. 2017 Mar 23;8:14771
pubmed: 28332494