A correlation study between virulence factors and multidrug resistance among clinical isolates of Proteus mirabilis.
Biofilm formation in P. mirabilis
Multidrug resistant P. mirabilis
P. mirabilis virulence factors
P. mirabilis virulence genes
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
07
11
2022
accepted:
22
07
2023
medline:
11
9
2023
pubmed:
3
8
2023
entrez:
3
8
2023
Statut:
ppublish
Résumé
Treatment of Proteus mirabilis infections is a challenge due to the high abundance of virulence factors and the high intrinsic resistance to antimicrobials. Multidrug resistance (MDR) and extensive drug resistance (XDR) further challenge the control of P. mirabilis infection. This study aimed to investigate the correlation between virulence determinants and multidrug resistance in 100 clinical isolates of P. mirabilis collected in Alexandria from December 2019 to June 2021. Susceptibility to antimicrobials was tested by the Kirby Bauer method. Detection of swarming, urease, protease, hemolysin, and biofilm formation was performed phenotypically and by PCR amplification of zapA, flaA, ureC, mrpA, atfA, ucaA, hpmA, and luxS. MDR and XDR were detected in 34% and 5%, respectively. All isolates were positive for motility, swarming, urease, and protease production. Ninety percent were positive for hemolysin production, while 73% formed biofilm. All isolates possessed the ureC and zapA genes. The luxS, flaA, ucaA, hpmA, mrpA, and atfA genes were detected in 99%, 98%, 96% 90%, 89%, and 84%, respectively. The presence of a single biofilm-related gene was statistically correlated with non-biofilm production (P= 0.018). It was concluded that P. mirabilis isolates from catheterized-urine samples were significantly associated with biofilm formation. MDR and virulence were not statistically correlated. A significant positive correlation was detected between some virulence genes in P. mirabilis. Non-MDR isolates of P. mirabilis had a high abundance of virulence factors with no statistically significant difference from MDR. Most of the MDR and all XDR isolates could produce biofilm.
Identifiants
pubmed: 37535261
doi: 10.1007/s42770-023-01080-5
pii: 10.1007/s42770-023-01080-5
pmc: PMC10484824
doi:
Substances chimiques
Virulence Factors
0
3-((aminoiminomethyl)thio)-2-propenoic acid
92138-10-8
hydroxypropyl methacrylate
UKW89XAX2X
Hemolysin Proteins
0
Urease
EC 3.5.1.5
Peptide Hydrolases
EC 3.4.-
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1387-1397Informations de copyright
© 2023. The Author(s).
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