RNA helicase DHX33 regulates HMGB family genes in human cancer cells.
DHX33
Epigenetic
HMGB
RNA helicase
cancer
Journal
Cellular signalling
ISSN: 1873-3913
Titre abrégé: Cell Signal
Pays: England
ID NLM: 8904683
Informations de publication
Date de publication:
10 2023
10 2023
Historique:
received:
12
05
2023
revised:
26
07
2023
accepted:
28
07
2023
medline:
28
8
2023
pubmed:
6
8
2023
entrez:
5
8
2023
Statut:
ppublish
Résumé
RNA helicase DHX33 has been shown to be aberrantly expressed in various human cancers, however, its role in tumorigenesis remains incompletely understood. In this report, we uncovered that a family of DNA architecture proteins, HMGBs, can be regulated by DHX33 in cancer cells but not in normal cells. Specifically, DHX33 knockdown caused the downregulation of HMGBs at the levels of both gene transcription and protein expression. Notably, in RAS driven lung tumorigenesis, nuclear HMGBs proteins can be induced via DHX33. When DHX33 was knocked out, HMGBs overexpression was debilitated. Mechanistically, DHX33 was found to bind to the promoters of HMGB family genes and regulated their transcription through demethylation on gene promoters. Our study reveals a novel mechanism for DHX33 to promote tumorigenesis and highlights its therapeutic value in human cancers.
Identifiants
pubmed: 37543097
pii: S0898-6568(23)00246-2
doi: 10.1016/j.cellsig.2023.110832
pii:
doi:
Substances chimiques
HMGB Proteins
0
DHX33 protein, human
EC 3.6.1.-
DEAD-box RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110832Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no conflicts of interest with the contents of this article.