The influence of calcitriol and methylprednisolone on podocytes function in minimal change disease in vitro model.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 08 2023
05 08 2023
Historique:
received:
13
04
2023
accepted:
01
08
2023
medline:
7
8
2023
pubmed:
6
8
2023
entrez:
5
8
2023
Statut:
epublish
Résumé
Minimal change disease (MCD), considered one of the major causes of nephrotic syndrome, is a complex pathological condition with disturbances in podocytes' foot processes. Numerous studies suggested the essential role of vitamin D3 in maintaining proper glomerulus function. However, the data on direct potential of that compound in reference to podocytes are scarce. Thus, here we assessed the influence of calcitriol (active vitamin D3) on podocyte function, apart from commonly used steroids (methylprednisolone). CIHP-1 podocyte cell line was used to implement the LPS-PAN-induced MCD in vitro model. Viability, podocyte-related slit diaphragm proteins, morphology, function as a barrier was evaluated using flow cytometry, RT-PCR, confocal microscopy, and TEER analysis. Calcitriol or methylprednisolone did not affect cell viability. Podocyte-related proteins demonstrated different responses to in vitro treatment compared to previously reported changes in total glomeruli. Podocyte morphology was partially restored in the presence of the tested compounds. In addition, TEER analysis revealed improvement of LPS-PAN-induced cells' function as a barrier when vitamin D3 or steroid was used. In conclusion, a significant potential for modulation of MCD in vitro model podocytes with calcitriol or selected steroids was reported. Further studies on vitamin D3 in context of podocyte-related phenomenon accompanying MCD are of great importance.
Identifiants
pubmed: 37543700
doi: 10.1038/s41598-023-39893-x
pii: 10.1038/s41598-023-39893-x
pmc: PMC10404287
doi:
Substances chimiques
Calcitriol
FXC9231JVH
Methylprednisolone
X4W7ZR7023
Lipopolysaccharides
0
Cholecalciferol
1C6V77QF41
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12731Informations de copyright
© 2023. Springer Nature Limited.
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