New insights into RNA processing by the eukaryotic tRNA splicing endonuclease.
RNA
RNA degradation
RNA processing
TSEN
protein structure
tRNA splicing
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
received:
14
03
2023
revised:
27
07
2023
accepted:
29
07
2023
medline:
2
10
2023
pubmed:
7
8
2023
entrez:
6
8
2023
Statut:
ppublish
Résumé
Through its role in intron cleavage, tRNA splicing endonuclease (TSEN) plays a critical function in the maturation of intron-containing pre-tRNAs. The catalytic mechanism and core requirement for this process is conserved between archaea and eukaryotes, but for decades, it has been known that eukaryotic TSENs have evolved additional modes of RNA recognition, which have remained poorly understood. Recent research identified new roles for eukaryotic TSEN, including processing or degradation of additional RNA substrates, and determined the first structures of pre-tRNA-bound human TSEN complexes. These recent discoveries have changed our understanding of how the eukaryotic TSEN targets and recognizes substrates. Here, we review these recent discoveries, their implications, and the new questions raised by these findings.
Identifiants
pubmed: 37544645
pii: S0021-9258(23)02166-X
doi: 10.1016/j.jbc.2023.105138
pmc: PMC10485636
pii:
doi:
Substances chimiques
RNA Precursors
0
RNA, Transfer
9014-25-9
splicing endonuclease
EC 3.1.27.-
Endoribonucleases
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
105138Subventions
Organisme : NIGMS NIH HHS
ID : R00 GM143534
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM141710
Pays : United States
Organisme : NCATS NIH HHS
ID : R42 TR001711
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA ES103247
Pays : United States
Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflicts of interest The authors declare that they have no conflicts of interest with the contents of this article.