Self-assembled multienzyme complex facilitates synthesis of glucosylglycerol from maltodextrin and glycerol.
SpyTag-SpyCatcher
glucosylglycerol
maltodextrin
multienzyme complex
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
15 Jan 2024
15 Jan 2024
Historique:
revised:
03
08
2023
received:
09
05
2023
accepted:
08
08
2023
medline:
20
11
2023
pubmed:
8
8
2023
entrez:
8
8
2023
Statut:
ppublish
Résumé
Compound 2-O-α-d-glucosylglycerol (2αGG) naturally serves as a compatible osmolyte in acclimation to environmental stresses, such as high osmolarity, dryness, and extreme temperature. It presents several bioactivities and has been used in the food, agriculture, and cosmetics areas. In the present study, we attempted to synthesize the 2αGG from low-cost maltodextrin and glycerol by constructing an in vitro multi-enzyme system. The system contained two core enzymes, namely glucan phosphorylases (GPs) and glucosylglycerol phosphorylases (GGPs), and two auxiliary enzymes, namely isoamylase and 4-α-glucanotransferase. Several new GGPs from different organisms were characterized with the function of converting α-G1P and glycerol to sole stereo-configuration product 2αGG. Then, polypeptide SpyTag-SpyCatcher was employed to construct a self-assembled multienzyme complex, and different combinations between enzymes and peptides were constructed and tested. The best self-assembled multienzyme complex exhibited three-fold higher productivity compared to that of free enzyme. This reaction system also produced 240 mm (61 g L The present study provides an efficient approach for producing 2αGG. It also demonstrates that the SpyTag-SpyCatcher system could be applied to construct other multienzyme complexes for increased productivity and product titer. © 2023 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Compound 2-O-α-d-glucosylglycerol (2αGG) naturally serves as a compatible osmolyte in acclimation to environmental stresses, such as high osmolarity, dryness, and extreme temperature. It presents several bioactivities and has been used in the food, agriculture, and cosmetics areas.
RESULTS
RESULTS
In the present study, we attempted to synthesize the 2αGG from low-cost maltodextrin and glycerol by constructing an in vitro multi-enzyme system. The system contained two core enzymes, namely glucan phosphorylases (GPs) and glucosylglycerol phosphorylases (GGPs), and two auxiliary enzymes, namely isoamylase and 4-α-glucanotransferase. Several new GGPs from different organisms were characterized with the function of converting α-G1P and glycerol to sole stereo-configuration product 2αGG. Then, polypeptide SpyTag-SpyCatcher was employed to construct a self-assembled multienzyme complex, and different combinations between enzymes and peptides were constructed and tested. The best self-assembled multienzyme complex exhibited three-fold higher productivity compared to that of free enzyme. This reaction system also produced 240 mm (61 g L
CONCLUSION
CONCLUSIONS
The present study provides an efficient approach for producing 2αGG. It also demonstrates that the SpyTag-SpyCatcher system could be applied to construct other multienzyme complexes for increased productivity and product titer. © 2023 Society of Chemical Industry.
Substances chimiques
maltodextrin
7CVR7L4A2D
glucosylglycerol
0
Glycerol
PDC6A3C0OX
Polysaccharides
0
Phosphorylases
EC 2.4.1.-
Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
266-272Subventions
Organisme : National Key R&D Program of China
ID : 2022YFC2104901
Organisme : National Natural Science Foundation of China
ID : 32271545
Organisme : National Natural Science Foundation of China
ID : 31972030
Organisme : Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project
ID : TSBICIP-CXRC-047
Organisme : Youth Promotion Association of Chinese Academy of Sciences
ID : 2021176
Informations de copyright
© 2023 Society of Chemical Industry.
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