A mosaic adeno-associated virus vector as a versatile tool that exhibits high levels of transgene expression and neuron specificity in primate brain.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
08 08 2023
08 08 2023
Historique:
received:
06
08
2021
accepted:
27
07
2023
medline:
10
8
2023
pubmed:
9
8
2023
entrez:
8
8
2023
Statut:
epublish
Résumé
Recent emphasis has been placed on gene transduction mediated through recombinant adeno-associated virus (AAV) vector to manipulate activity of neurons and their circuitry in the primate brain. In the present study, we created a novel vector of which capsid was composed of capsid proteins derived from both of the AAV serotypes 1 and 2 (AAV1 and AAV2). Following the injection into the frontal cortex of macaque monkeys, this mosaic vector, termed AAV2.1 vector, was found to exhibit the excellence in transgene expression (for AAV1 vector) and neuron specificity (for AAV2 vector) simultaneously. To explore its applicability to chemogenetic manipulation and in vivo calcium imaging, the AAV2.1 vector expressing excitatory DREADDs or GCaMP was injected into the striatum or the visual cortex of macaque monkeys, respectively. Our results have defined that such vectors secure intense and stable expression of the target proteins and yield conspicuous modulation and imaging of neuronal activity.
Identifiants
pubmed: 37553329
doi: 10.1038/s41467-023-40436-1
pii: 10.1038/s41467-023-40436-1
pmc: PMC10409865
doi:
Substances chimiques
Capsid Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4762Informations de copyright
© 2023. Springer Nature Limited.
Références
Mol Ther. 2014 Feb;22(2):329-337
pubmed: 24419081
Nat Neurosci. 2012 Oct;15(10):1368-70
pubmed: 22941109
Neuron. 2012 Dec 6;76(5):901-7
pubmed: 23217739
J Psychopharmacol. 2020 Dec;34(12):1418-1430
pubmed: 33200659
Lancet. 2017 Aug 26;390(10097):849-860
pubmed: 28712537
Mol Ther. 2010 Mar;18(3):588-93
pubmed: 20010918
Nat Neurosci. 2020 Sep;23(9):1157-1167
pubmed: 32632286
Hum Mol Genet. 2019 Oct 1;28(R1):R3-R14
pubmed: 31261383
Nature. 2004 Jan 29;427(6973):448-51
pubmed: 14749833
Science. 2017 Aug 18;357(6352):687-692
pubmed: 28818944
Neuron. 2017 Jan 4;93(1):33-47
pubmed: 27989459
Hum Gene Ther. 2020 Feb;31(3-4):151-162
pubmed: 31914802
J Nucl Med. 1996 Mar;37(3):513-20
pubmed: 8772658
Mol Ther. 2011 Jun;19(6):1058-69
pubmed: 21487395
Nat Commun. 2016 Dec 06;7:13605
pubmed: 27922009
Mol Ther. 2003 Mar;7(3):419-25
pubmed: 12668138
Nat Protoc. 2010 Mar;5(3):439-56
pubmed: 20203662
Curr Opin Virol. 2016 Dec;21:75-80
pubmed: 27596608
Physiol Rev. 2018 Jan 1;98(1):391-418
pubmed: 29351511
Nat Rev Neurosci. 2012 Mar 20;13(4):251-66
pubmed: 22430017
Gene Ther. 2001 Jun;8(11):864-73
pubmed: 11423934
J Virol. 2004 May;78(9):4421-32
pubmed: 15078923
Exp Neurol. 2008 Jan;209(1):51-7
pubmed: 17920059
Ann Neurol. 1979 Nov;6(5):371-88
pubmed: 117743
Mol Ther. 2008 Feb;16(2):296-301
pubmed: 18223548
Science. 2009 Apr 17;324(5925):354-9
pubmed: 19299587
Exp Neurol. 2012 Feb;233(2):807-14
pubmed: 22177996
Neuron. 2009 Jul 16;63(1):27-39
pubmed: 19607790
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Mol Ther. 2020 Mar 4;28(3):723-746
pubmed: 31972133
Curr Biol. 2012 Sep 25;22(18):1722-6
pubmed: 22840516
Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5163-8
pubmed: 17360345
Neuron. 2017 Mar 8;93(5):1049-1057.e3
pubmed: 28215557
Mol Ther. 2000 Apr;1(4):376-82
pubmed: 10933956
Neuron. 2017 Jul 5;95(1):51-62.e4
pubmed: 28648497
J Neurosci. 2013 Oct 16;33(42):16818-27
pubmed: 24133282
Mol Ther. 2020 Mar 4;28(3):709-722
pubmed: 31968213
J Clin Invest. 2013 Dec;123(12):5342-50
pubmed: 24231358
Neuroimage. 2018 Oct 15;180(Pt A):312-323
pubmed: 29331450
Mol Ther. 2013 Jan;21(1):158-66
pubmed: 22929660
Nature. 2007 Nov 15;450(7168):420-4
pubmed: 17943086
Sci Rep. 2019 Mar 5;9(1):3567
pubmed: 30837514
Hum Gene Ther. 2009 Mar;20(3):225-37
pubmed: 19292604
Neurosci Res. 2015 Apr;93:144-57
pubmed: 25240284
Hum Gene Ther. 1998 Feb 10;9(3):287-93
pubmed: 9508046
J Neurosci. 2017 Nov 8;37(45):10894-10903
pubmed: 29118219
Cereb Cortex. 2019 Feb 1;29(2):666-679
pubmed: 29329408
Neuron. 2016 Feb 17;89(4):683-94
pubmed: 26889809
Neuron. 2016 Apr 6;90(1):143-51
pubmed: 27021172
Mol Ther. 2008 Jun;16(6):1073-80
pubmed: 18414476
Cell. 2016 Sep 8;166(6):1564-1571.e6
pubmed: 27610576
Nat Commun. 2015 Sep 21;6:8378
pubmed: 26387804
J Neurosci. 2013 Oct 16;33(42):16684-97
pubmed: 24133271
Curr Gene Ther. 2005 Jun;5(3):299-310
pubmed: 15975007
J Physiol. 2019 Oct;597(19):5025-5040
pubmed: 31397900
Gene Ther. 2008 Jun;15(11):858-63
pubmed: 18418415