Quality Control of Chemogenomic Library Using LC-MS.

Chromatography, Liquid Tandem Mass Spectrometry Quality Control
Chemical integrity Chemogenomic library Identity LC-MS Liquid chromatography Mass spectrometry Purity Qualitative analysis Quantitative analysis

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
medline: 11 8 2023
pubmed: 10 8 2023
entrez: 9 8 2023
Statut: ppublish

Résumé

In chemical biology, using compounds with incorrect identity or insufficient purity can lead to misleading biological activity data. Chemical quality control for confirmation of purity and compound identity is thus central to chemogenomics. We have established a medium-throughput LC-MS-based semi-automated quality control (QC) workflow with a minimal requirement for materials suitable for chemogenomics and other small molecule libraries. This rapid method can cover a broad chemical space of small organic compounds with diverse physicochemical properties such as polarity or lipophilicity.

Identifiants

DOI: 10.1007/978-1-0716-3397-7_4 PMID: 37558940
pubmed: 37558940
doi: 10.1007/978-1-0716-3397-7_4
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

51-58

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Begou O, Gika HG, Theodoridis GA, Wilson ID (2018) Quality control and validation issues in LC-MS metabolomics. In: Theodoridis GA, Gika HG, Wilson ID (eds) Metabolic profiling, Methods in molecular biology, vol 1738. Springer, New York, pp 15–26. https://doi.org/10.1007/978-1-4939-7643-0_2
doi: 10.1007/978-1-4939-7643-0_2
Korfmacher WA (2005) Foundation review: principles and applications of LC-MS in new drug discovery. Drug Discov Today 10(20):1357–1367. https://doi.org/10.1016/S1359-6446(05)03620-2
doi: 10.1016/S1359-6446(05)03620-2 pubmed: 16253874
Niessen WMA (2003) Progress in liquid chromatography–mass spectrometry instrumentation and its impact on high-throughput screening. J Chromatogr A 1000(1–2):413–436. https://doi.org/10.1016/S0021-9673(03)00506-5
doi: 10.1016/S0021-9673(03)00506-5 pubmed: 12877182

Auteurs

Václav Němec (V)

Institute for Pharmaceutical Chemistry, Structural Genomics Consortium, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany.

Stefan Knapp (S)

Institute for Pharmaceutical Chemistry, Structural Genomics Consortium, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany. knapp@pharmchem.uni-frankfurt.de.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Quality Control of Chemogenomic Library Using LC-MS.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Quality Control of Chemogenomic Library Using LC-MS.
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Contrôle de qualité › Quality Control of Chemogenomic Library Using LC-MS.