Enhancement of capsular hypermucoviscosity in Klebsiella pneumoniae by Acanthamoeba.
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
received:
20
04
2023
accepted:
18
07
2023
revised:
11
09
2023
medline:
12
9
2023
pubmed:
11
8
2023
entrez:
11
8
2023
Statut:
epublish
Résumé
Acanthamoeba and Klebsiella pneumoniae are both environmental commensals. Recently, clinical harm caused by hypermucoviscous K. pneumoniae has been observed. However, the interaction between these microbes and the origin of hypermucoviscous K. pneumoniae have not been reported. Here, we report that the bacterial capsule is enlarged when co-cultured with Acanthamoeba using India ink staining, and this effect depends on the number of parasites present. This interaction results in an enhancement of capsular polysaccharide production in the subsequent generations of K. pneumoniae, even without co-culturing with Acanthamoeba. The hypermucoviscosity of the capsule was examined using the sedimentation assay and string test. We also screened other K. pneumoniae serotypes, including K1, K2, K5, and K20, for interaction with Acanthamoeba using India ink staining, and found the same interaction effect. These findings suggest that the interaction between Acanthamoeba and K. pneumoniae could lead to harmful consequences in public health and nosocomial disease control, particularly hypermucoviscous K. pneumoniae infections.
Sections du résumé
BACKGROUND
Acanthamoeba and Klebsiella pneumoniae are both environmental commensals. Recently, clinical harm caused by hypermucoviscous K. pneumoniae has been observed. However, the interaction between these microbes and the origin of hypermucoviscous K. pneumoniae have not been reported.
METHODOLOGY/PRINCIPAL FINDINGS
Here, we report that the bacterial capsule is enlarged when co-cultured with Acanthamoeba using India ink staining, and this effect depends on the number of parasites present. This interaction results in an enhancement of capsular polysaccharide production in the subsequent generations of K. pneumoniae, even without co-culturing with Acanthamoeba. The hypermucoviscosity of the capsule was examined using the sedimentation assay and string test. We also screened other K. pneumoniae serotypes, including K1, K2, K5, and K20, for interaction with Acanthamoeba using India ink staining, and found the same interaction effect.
CONCLUSIONS/SIGNIFICANCE
These findings suggest that the interaction between Acanthamoeba and K. pneumoniae could lead to harmful consequences in public health and nosocomial disease control, particularly hypermucoviscous K. pneumoniae infections.
Identifiants
pubmed: 37566587
doi: 10.1371/journal.pntd.0011541
pii: PNTD-D-23-00485
pmc: PMC10495012
doi:
Substances chimiques
chinese ink
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0011541Informations de copyright
Copyright: © 2023 Huang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Anal Biochem. 1973 Aug;54(2):484-9
pubmed: 4269305
ISME J. 2009 Jun;3(6):675-84
pubmed: 19242534
Molecules. 2017 Dec 18;22(12):
pubmed: 29258252
IDCases. 2019 May 03;17:e00547
pubmed: 31193033
J Bacteriol. 1991 Mar;173(5):1738-47
pubmed: 1999391
Clin Microbiol Rev. 1998 Oct;11(4):589-603
pubmed: 9767057
Parasitol Res. 2013 Nov;112(11):3687-96
pubmed: 23933810
Clin Infect Dis. 2006 May 15;42(10):1351-8
pubmed: 16619144
Infect Immun. 2006 Sep;74(9):5402-7
pubmed: 16926436
Infect Control. 1985 Feb;6(2):52-8
pubmed: 3882590
Infect Immun. 1989 Dec;57(12):3778-82
pubmed: 2680983
Ophthalmology. 2010 Mar;117(3):445-52, 452.e1-3
pubmed: 20031220
J Clin Microbiol. 1984 Nov;20(5):936-41
pubmed: 6392324
Antimicrob Agents Chemother. 2020 Apr 21;64(5):
pubmed: 32152079
Infect Disord Drug Targets. 2017;17(3):160-177
pubmed: 28637420
Am J Gastroenterol. 2005 Feb;100(2):322-31
pubmed: 15667489
Parasitol Res. 2017 Nov;116(11):3151-3162
pubmed: 28988383
FEMS Microbiol Rev. 2010 May;34(3):231-59
pubmed: 19744244
Infect Immun. 1992 Jan;60(1):296-301
pubmed: 1729191
Microorganisms. 2022 Sep 23;10(10):
pubmed: 36296171
Emerg Microbes Infect. 2021 Dec;10(1):277-290
pubmed: 33538648
Trans R Soc Trop Med Hyg. 2014 Jun;108(6):326-37
pubmed: 24781376
Trends Parasitol. 2022 Nov;38(11):975-990
pubmed: 36109313
Parasit Vectors. 2019 Oct 9;12(1):467
pubmed: 31597577
Exp Parasitol. 2013 Sep;135(1):30-5
pubmed: 23769934
Emerg Infect Dis. 2006 Feb;12(2):248-55
pubmed: 16494750
Appl Environ Microbiol. 2008 Dec;74(23):7183-8
pubmed: 18849458
Infect Immun. 1994 Aug;62(8):3254-61
pubmed: 8039895
BMC Infect Dis. 2022 Sep 29;22(1):757
pubmed: 36175838
mBio. 2015 Jun 09;6(3):e00775
pubmed: 26060277
Microbiology (Reading). 2009 Dec;155(Pt 12):4170-4183
pubmed: 19744990
Environ Microbiol. 2008 Oct;10(10):2728-45
pubmed: 18637950
J Bacteriol. 2010 Jun;192(12):3144-58
pubmed: 20382770
Parasit Vectors. 2019 Nov 14;12(1):538
pubmed: 31727139
mSphere. 2018 Nov 7;3(6):
pubmed: 30404929
Molecules. 2022 Sep 26;27(19):
pubmed: 36234875
Molecules. 2019 Dec 13;24(24):
pubmed: 31847255
Microorganisms. 2020 Oct 10;8(10):
pubmed: 33050506
J Clin Pathol. 1980 Dec;33(12):1179-83
pubmed: 7451664
Microbiol Mol Biol Rev. 2016 Jun 15;80(3):629-61
pubmed: 27307579
Front Microbiol. 2021 Jan 07;11:616213
pubmed: 33488560
Pathogens. 2021 Feb 25;10(3):
pubmed: 33669045
PLoS One. 2013 Dec 09;8(12):e80670
pubmed: 24349011
Virulence. 2013 Feb 15;4(2):107-18
pubmed: 23302790
Emerg Infect Dis. 2009 Jul;15(7):1144-6
pubmed: 19624948
Curr Microbiol. 2017 May;74(5):541-549
pubmed: 28258293