Structural basis for high-order complex of SARNP and DDX39B to facilitate mRNP assembly.

Humans Nuclear Proteins / metabolism Ribonucleoproteins / metabolism Transcription Factors / metabolism RNA, Messenger / metabolism Saccharomyces cerevisiae / metabolism DEAD-box RNA Helicases / metabolism
CP: Molecular biology DDX39B DEAD-box ATPase SARNP Sub2 TREX complex Tho1 UAP56 mRNA nuclear export mRNP assembly

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
29 08 2023
Historique:
received: 05 12 2022
revised: 10 04 2023
accepted: 31 07 2023
medline: 4 9 2023
pubmed: 14 8 2023
entrez: 14 8 2023
Statut: ppublish

Résumé

mRNA in eukaryotic cells is packaged into highly compacted ribonucleoprotein particles (mRNPs) in the nucleus and exported to the cytoplasm for translation. mRNP packaging and export require the evolutionarily conserved transcription-export (TREX) complex. TREX facilitates loading of various RNA-binding proteins on mRNA through the action of its DDX39B subunit. SARNP (Tho1 [transcriptional defect of Hpr1 by overexpression 1] in yeast) is shown to interact with DDX39B and affect mRNA export. The molecular mechanism of how SARNP recognizes DDX39B and functions in mRNP assembly is unclear. Here, we determine the crystal structure of a Tho1/DDX39B/RNA complex, revealing a multivalent interaction mediated by tandem DDX39B interacting motifs in SARNP/Tho1. The high-order complex of SARNP and DDX39B is evolutionarily conserved, and human SARNP can engage with five DDX39B molecules. RNA sequencing (RNA-seq) from SARNP knockdown cells shows the most affected RNAs in export are GC rich. Our work suggests the role of the high-order SARNP/DDX39B/RNA complex in mRNP assembly and export.

Identifiants

DOI: 10.1016/j.celrep.2023.112988 PMID: 37578863 PMC: PMC10508174
pubmed: 37578863
pii: S2211-1247(23)00999-3
doi: 10.1016/j.celrep.2023.112988
pmc: PMC10508174
mid: NIHMS1928288
pii:
doi:

Substances chimiques

messenger ribonucleoprotein 0
Nuclear Proteins 0
Ribonucleoproteins 0
Transcription Factors 0
RNA, Messenger 0
SARNP protein, human 0
DDX39B protein, human EC 3.6.1.-
DEAD-box RNA Helicases EC 3.6.4.13

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

112988

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI125524
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI154635
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM133743
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA119925
Pays : United States

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Yihu Xie (Y)

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA; Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA.

Shengyan Gao (S)

Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9039, USA.

Ke Zhang (K)

Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9039, USA.

Prasanna Bhat (P)

Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9039, USA.

Bradley P Clarke (BP)

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA; Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA.

Kimberly Batten (K)

Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9039, USA.

Menghan Mei (M)

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA; Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA.

Matthew Gazzara (M)

Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA.

Jerry W Shay (JW)

Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9039, USA.

Kristen W Lynch (KW)

Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA.

Alexia E Angelos (AE)

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA; Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA.

Pate S Hill (PS)

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA; Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA.

Austin L Ivey (AL)

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA; Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA.

Beatriz M A Fontoura (BMA)

Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9039, USA. Electronic address: beatriz.fontoura@utsouthwestern.edu.

Yi Ren (Y)

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA; Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA. Electronic address: yi.ren@vanderbilt.edu.

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