Exploring the impact of nucleic acids on protein stability in bacterial cell lysate.
Anti-aggregation activity
Chaperone
DNA
Nucleic acids
RNA
Journal
Biochimica et biophysica acta. General subjects
ISSN: 1872-8006
Titre abrégé: Biochim Biophys Acta Gen Subj
Pays: Netherlands
ID NLM: 101731726
Informations de publication
Date de publication:
10 2023
10 2023
Historique:
received:
24
04
2023
revised:
02
08
2023
accepted:
14
08
2023
medline:
18
9
2023
pubmed:
18
8
2023
entrez:
17
8
2023
Statut:
ppublish
Résumé
- Addition of salt enhanced thermal stability of model substrate proteins by reducing electrostatic repulsion between protein molecules. - However, the opposite effect was observed with bacterial cell lysate, indicating that certain molecules within the lysate could enhance protein stability via electrostatic interactions. - Such molecules present in cell lysate were found to be nucleic acids known to have a potent anti-aggregation activity toward proteins involving electrostatic interactions. - Nucleic acids showed chaperone activity in physiological salt concentration within cells and in buffer or medium commonly used in experiments. - The chaperone activity of nucleic acids should be taken into account when performing various in vitro assays using cell lysate or samples containing nucleic acids.
Identifiants
pubmed: 37591417
pii: S0304-4165(23)00143-5
doi: 10.1016/j.bbagen.2023.130445
pii:
doi:
Substances chimiques
Nucleic Acids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
130445Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Changhan Lee reports financial support was provided by National Research Foundation of Korea (NRF) funded by the Korea government (MSIT). Changhan Lee reports financial support was provided by Basic Science Research Program through the NRF funded by the Ministry of Education. Changhan Lee reports financial support was provided by the new faculty research fund of Ajou University.