Development and application of a visualization method for identification of Panax species with LAMP and a DNAzyme.

DNA, Catalytic Hydrogen Peroxide Chromatography, Gas Computer Simulation Panax
DNAzyme LAMP Panax ginseng Panax quinquefolium Visual identification

Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
15 10 2023
Historique:
received: 05 06 2023
revised: 13 08 2023
accepted: 21 08 2023
medline: 8 9 2023
pubmed: 25 8 2023
entrez: 24 8 2023
Statut: ppublish

Résumé

Panax ginseng and Panax quinquefolium are two valuable Chinese herbal medicines that should not be mixed because they differ in drug properties and efficacy. The traditional identification method is easily affected by subjective factors and cannot effectively distinguish between ginseng products. This study aimed to develop a new chemical analysis method to visually identify P. ginseng and P. quinquefolium. In this method, a large number of sequences containing G-quadruplex were generated by loop-mediated isothermal amplification, and the combination of G-quadruplex and hemin was used to form deoxyribozyme, which catalyzed the color change of H

Identifiants

DOI: 10.1016/j.ab.2023.115298 PMID: 37619904
pubmed: 37619904
pii: S0003-2697(23)00263-4
doi: 10.1016/j.ab.2023.115298
pii:
doi:

Substances chimiques

DNA, Catalytic 0
Hydrogen Peroxide BBX060AN9V

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

115298

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest All authors disclosed no relevant relationships.

Auteurs

Xiangjun Wang (X)

College of Medical Technology, Beihua University, Jilin, 132000, China.

Moyi Liu (M)

College of Medical Technology, Beihua University, Jilin, 132000, China.

Ying Li (Y)

College of Medical Technology, Beihua University, Jilin, 132000, China.

Xinchen Zhou (X)

College of Medical Technology, Beihua University, Jilin, 132000, China.

Zhuo Zhang (Z)

College of Medical Technology, Beihua University, Jilin, 132000, China.

Shuhan Dong (S)

College of Medical Technology, Beihua University, Jilin, 132000, China.

Mingmei Shen (M)

College of Medical Technology, Beihua University, Jilin, 132000, China.

Minghui Wang (M)

College of Medical Technology, Beihua University, Jilin, 132000, China.

Helin Wang (H)

College of Medical Technology, Beihua University, Jilin, 132000, China.

Limei Liu (L)

College of Medical Technology, Beihua University, Jilin, 132000, China. Electronic address: liulm74@163.com.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN catalytique › Development and application of a visualization...
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Peroxydes › Peroxyde d'hydrogène › Development and application of a visualization...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase gazeuse › Development and application of a visualization...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Development and application of a visualization...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Araliaceae › Panax › Development and application of a visualization...