Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells.
airway basal cells
airway epithelial stem cell transplantation
directed differentiation
pluripotent stem cells
regeneration
Journal
Cell stem cell
ISSN: 1875-9777
Titre abrégé: Cell Stem Cell
Pays: United States
ID NLM: 101311472
Informations de publication
Date de publication:
07 09 2023
07 09 2023
Historique:
received:
31
10
2022
revised:
13
06
2023
accepted:
28
07
2023
medline:
11
9
2023
pubmed:
26
8
2023
entrez:
25
8
2023
Statut:
ppublish
Résumé
Life-long reconstitution of a tissue's resident stem cell compartment with engrafted cells has the potential to durably replenish organ function. Here, we demonstrate the engraftment of the airway epithelial stem cell compartment via intra-airway transplantation of mouse or human primary and pluripotent stem cell (PSC)-derived airway basal cells (BCs). Murine primary or PSC-derived BCs transplanted into polidocanol-injured syngeneic recipients give rise for at least two years to progeny that stably display the morphologic, molecular, and functional phenotypes of airway epithelia. The engrafted basal-like cells retain extensive self-renewal potential, evident by the capacity to reconstitute the tracheal epithelium through seven generations of secondary transplantation. Using the same approach, human primary or PSC-derived BCs transplanted into NOD scid gamma (NSG) recipient mice similarly display multilineage airway epithelial differentiation in vivo. Our results may provide a step toward potential future syngeneic cell-based therapy for patients with diseases resulting from airway epithelial cell damage or dysfunction.
Identifiants
pubmed: 37625411
pii: S1934-5909(23)00281-3
doi: 10.1016/j.stem.2023.07.014
pmc: PMC10528754
mid: NIHMS1928352
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1199-1216.e7Subventions
Organisme : NICHD NIH HHS
ID : R21 HD094012
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL007035
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL134745
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL139799
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL095993
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL124392
Pays : United States
Organisme : NHLBI NIH HHS
ID : U24 HL134763
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK065988
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL134766
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL148692
Pays : United States
Organisme : NHLBI NIH HHS
ID : 75N92020C00005
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors have filed a patent application related to the engraftment approach presented in this manuscript.
Références
Methods Mol Biol. 2013;945:109-21
pubmed: 23097104
Dis Model Mech. 2010 Sep-Oct;3(9-10):545-56
pubmed: 20699479
Cell Stem Cell. 2020 Mar 5;26(3):346-358.e4
pubmed: 31978363
Cell. 2004 Sep 3;118(5):635-48
pubmed: 15339667
Dev Cell. 2018 Mar 26;44(6):752-761.e4
pubmed: 29587145
Stem Cell Reports. 2018 Jan 9;10(1):101-119
pubmed: 29249664
Am J Respir Crit Care Med. 2016 Jul 15;194(2):156-68
pubmed: 26840431
Sci Rep. 2016 Feb 04;6:20270
pubmed: 26842779
Sci Adv. 2017 Aug 30;3(8):e1700521
pubmed: 28875163
Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12771-5
pubmed: 19625615
Cell Stem Cell. 2015 Nov 5;17(5):527-42
pubmed: 26593959
Cell Stem Cell. 2023 Sep 7;30(9):1217-1234.e7
pubmed: 37625412
J Cell Comp Physiol. 1964 Aug;64:23-31
pubmed: 14200349
Cell Rep. 2018 Oct 16;25(3):598-610.e5
pubmed: 30332641
Am J Respir Cell Mol Biol. 2001 Jun;24(6):662-70
pubmed: 11415930
Lancet. 1981 Jan 10;1(8211):75-8
pubmed: 6109123
Science. 1997 Apr 4;276(5309):60-6
pubmed: 9082986
Bioinformatics. 2018 Apr 1;34(7):1246-1248
pubmed: 29228172
Am J Respir Cell Mol Biol. 2017 Jan;56(1):1-10
pubmed: 27632244
Nature. 2017 Nov 16;551(7680):327-332
pubmed: 29144448
Cell Stem Cell. 2021 Jan 7;28(1):79-95.e8
pubmed: 33098807
Cell. 2021 Jun 24;184(13):3573-3587.e29
pubmed: 34062119
Cell Rep. 2020 Dec 29;33(13):108553
pubmed: 33378665
Exp Lung Res. 2013 Apr;39(3):146-54
pubmed: 23527783
Cell Stem Cell. 2016 Aug 4;19(2):217-231
pubmed: 27320041
Exp Lung Res. 2001 Jul-Aug;27(5):401-15
pubmed: 11480582
iScience. 2022 Apr 16;25(5):104262
pubmed: 35521516
Am J Respir Cell Mol Biol. 2017 May;56(5):568-574
pubmed: 27983869
Development. 2017 Nov 1;144(21):3879-3893
pubmed: 28947536
Sci Rep. 2018 Oct 17;8(1):15312
pubmed: 30333510
Nature. 2015 Jan 29;517(7536):621-5
pubmed: 25533958
Annu Rev Cell Dev Biol. 1995;11:35-71
pubmed: 8689561
Cell Stem Cell. 2017 Jun 1;20(6):844-857.e6
pubmed: 28366587
J Clin Invest. 2010 Jan;120(1):379-89
pubmed: 20038801
JCI Insight. 2022 Mar 22;7(6):
pubmed: 35315362
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
Nature. 2018 Aug;560(7718):377-381
pubmed: 30069046
Nat Protoc. 2019 Dec;14(12):3303-3332
pubmed: 31732721
Cell Rep. 2022 Apr 12;39(2):110662
pubmed: 35417699
Blood. 2020 Dec 17;136(25):2893-2904
pubmed: 32614947
Nucleic Acids Res. 2001 May 1;29(9):e45
pubmed: 11328886
Methods. 2019 Jul 15;164-165:18-28
pubmed: 31216442
JCI Insight. 2023 Jan 10;8(1):
pubmed: 36454643
Nat Cell Biol. 2022 Jan;24(1):10-23
pubmed: 34969962
Am J Physiol Lung Cell Mol Physiol. 2004 Apr;286(4):L643-9
pubmed: 12871857
Nature. 2007 Oct 25;449(7165):1003-7
pubmed: 17934449
Dev Cell. 2020 Apr 6;53(1):117-128.e6
pubmed: 32109386
N Engl J Med. 2010 Jul 8;363(2):147-55
pubmed: 20573916
Elife. 2013 Oct 22;2:e00966
pubmed: 24151545
Stem Cell Reports. 2015 Apr 14;4(4):569-77
pubmed: 25772471
Am J Respir Crit Care Med. 2014 Dec 15;190(12):1355-62
pubmed: 25354273
Cell Stem Cell. 2012 Apr 6;10(4):398-411
pubmed: 22482505
Cell Rep. 2015 Jul 7;12(1):90-101
pubmed: 26119728
Nature. 2021 Apr;592(7852):99-104
pubmed: 33627870
Stem Cells. 2023 Aug 16;41(8):809-820
pubmed: 37468433
Front Pharmacol. 2019 Feb 08;10:74
pubmed: 30800069
PLoS One. 2014 Feb 21;9(2):e88848
pubmed: 24586412
Nat Commun. 2020 Jan 31;11(1):635
pubmed: 32005814
Stem Cell Reports. 2018 May 8;10(5):1579-1595
pubmed: 29657097
Nature. 2004 May 6;429(6987):41-6
pubmed: 15129273
Genome Biol. 2015 Dec 10;16:278
pubmed: 26653891
Biotechnol Adv. 2014 Mar-Apr;32(2):504-13
pubmed: 24440487
J Clin Invest. 2013 Jul;123(7):3025-36
pubmed: 23921127
Nat Methods. 2017 Dec;14(12):1205-1212
pubmed: 29106405
J Clin Invest. 2017 Jun 1;127(6):2277-2294
pubmed: 28463226
Nature. 2018 Aug;560(7718):319-324
pubmed: 30069044
Stem Cell Reports. 2014 Oct 14;3(4):634-49
pubmed: 25358791