Preparation of pepsin trypsin digested gliadin for stimulation experiments.

Humans Gliadin Pepsin A Trypsin Celiac Disease Laboratories
Celiac disease Gliadin Pepsin-trypsin digestion Stimulation

Journal

Methods in cell biology
ISSN: 0091-679X
Titre abrégé: Methods Cell Biol
Pays: United States
ID NLM: 0373334

Informations de publication

Date de publication:
2023
Historique:
medline: 28 8 2023
pubmed: 26 8 2023
entrez: 25 8 2023
Statut: ppublish

Résumé

Celiac disease (CD) is a complex immune disorder of the intestine that developes in genetically susceptible individuals. CD develops as an intolerance to ingested gluten proteins (gliadins, secalins, hordeins and avenins), being gliadin one of the most immunogenic. Here we present a protocol for the preparation of digested gliadin for laboratory use, a fundamental axis for in vitro and in vivo stimulation studies related to celiac disease research. The importance of a scrupulous handling of materials, products and laboratory instruments to achieve a lipopolysaccharide free gliadin is explained and emphasized. Therefore, in the present chapter, a step-by-step set-up of the protocol for pepsin trypsin gliadin digestion is explained.

Identifiants

DOI: 10.1016/bs.mcb.2022.11.005 PMID: 37625875
pubmed: 37625875
pii: S0091-679X(22)00188-1
doi: 10.1016/bs.mcb.2022.11.005
pii:
doi:

Substances chimiques

Gliadin 9007-90-3
Pepsin A EC 3.4.23.1
Trypsin EC 3.4.21.4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

195-201

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Auteurs

Luis Manuel Mendoza-Gomez (LM)

Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, Leioa, Spain.

Maialen Sebastian-delaCruz (M)

Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, Leioa, Spain; Biocruces Bizkaia Health Research Institute, Barakaldo, Spain.

Ane Olazagoitia-Garmendia (A)

Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, Leioa, Spain; Biocruces Bizkaia Health Research Institute, Barakaldo, Spain.

Itziar González-Moro (I)

Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, Leioa, Spain; Biocruces Bizkaia Health Research Institute, Barakaldo, Spain.

Henar Rojas-Márquez (H)

Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, Leioa, Spain; Biocruces Bizkaia Health Research Institute, Barakaldo, Spain.

Jon Mentxaka (J)

Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, Leioa, Spain; Biocruces Bizkaia Health Research Institute, Barakaldo, Spain.

Izortze Santin (I)

Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, Leioa, Spain; Biocruces Bizkaia Health Research Institute, Barakaldo, Spain; CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain.

Ainara Castellanos-Rubio (A)

Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, Leioa, Spain; Biocruces Bizkaia Health Research Institute, Barakaldo, Spain; Ikerbasque, Basque Foundation for Science, Bilbao, Spain; CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain. Electronic address: ainara.castellanos@ehu.eus.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Preparation of pepsin trypsin digested gliadin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines de stockage des graines › Prolamines › Glutens › Gliadine › Preparation of pepsin trypsin digested gliadin...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Endopeptidases › Aspartic acid endopeptidases › Pepsine A › Preparation of pepsin trypsin digested gliadin...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Protéases à sérine › Serine endopeptidases › Trypsine › Preparation of pepsin trypsin digested gliadin...
questionsmedicales.fr › Maladies métaboliques et nutritionnelles › Maladies métaboliques › Syndromes de malabsorption › Maladie coeliaque › Preparation of pepsin trypsin digested gliadin...
questionsmedicales.fr › Établissements, main d'oeuvre et services de soins de santé › Établissements de santé › Laboratoires › Preparation of pepsin trypsin digested gliadin...