Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics.
IgG N-glycan library
carbohydrates
enzyme-catalyzed synthesis
functional glycomics
glycan microarray
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
10 2023
10 2023
Historique:
revised:
25
07
2023
received:
12
06
2023
medline:
30
10
2023
pubmed:
27
8
2023
entrez:
26
8
2023
Statut:
ppublish
Résumé
N-Glycosylation, a main post-translational modification of Immunoglobulin G (IgG), plays a significant role in modulating the immune functions of IgG. However, the precise function elucidation of IgG N-glycosylation remains impeded due to the obstacles in obtaining comprehensive and well-defined N-glycans. Here, an easy-to-implement divergent approach is described to synthesize a 64-membered IgG N-glycan library covering all possible biantennary and bisected N-glycans by reprogramming biosynthetic assembly lines based on the inherent branch selectivity and substrate specificity of enzymes. The unique binding specificities of 64 N-glycans with different proteins are deciphered by glycan microarray technology. This unprecedented collection of synthetic IgG N-glycans can serve as standards for N-glycan structure identification in complex biological samples and the microarray data enrich N-glycan glycomics to facilitate biomedical applications.
Identifiants
pubmed: 37632720
doi: 10.1002/advs.202303832
pmc: PMC10602528
doi:
Substances chimiques
Immunoglobulin G
0
Polysaccharides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2303832Subventions
Organisme : National Natural Science Foundation of China
ID : 22077130
Organisme : Science and Technology Commission of Shanghai Municipality
ID : 20ZR1467900
Organisme : CAS Pioneer Talent Program
Organisme : Shanghai Municipal Science and Technology Major Project
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
Références
Cell Chem Biol. 2019 Apr 18;26(4):535-547.e4
pubmed: 30745240
Glycobiology. 2006 Dec;16(12):21C-27C
pubmed: 16971379
Annu Rev Biochem. 2019 Jun 20;88:433-459
pubmed: 30917003
Proc Natl Acad Sci U S A. 2017 Oct 3;114(40):E8508-E8517
pubmed: 28923942
Nat Protoc. 2017 Aug;12(8):1702-1721
pubmed: 28749929
J Am Chem Soc. 2010 Jul 21;132(28):9653-62
pubmed: 20583754
ACS Cent Sci. 2021 Jul 28;7(7):1156-1165
pubmed: 34341769
Cell Host Microbe. 2017 Jan 11;21(1):23-34
pubmed: 28017661
Chem Sci. 2022 Jun 9;13(25):7644-7656
pubmed: 35872821
Angew Chem Int Ed Engl. 2016 Aug 22;55(35):10487-92
pubmed: 27443163
Proc Natl Acad Sci U S A. 2015 Aug 25;112(34):10611-6
pubmed: 26253764
Angew Chem Int Ed Engl. 2007;46(22):4173-5
pubmed: 17444542
Nat Rev Immunol. 2014 Oct;14(10):653-66
pubmed: 25234143
J Am Chem Soc. 2017 Jan 18;139(2):1011-1018
pubmed: 28002670
Annu Rev Biochem. 2011;80:797-823
pubmed: 21469953
Nat Rev Drug Discov. 2021 Mar;20(3):217-243
pubmed: 33462432
Angew Chem Int Ed Engl. 2013 Jul 15;52(29):7426-31
pubmed: 23740650
Adv Sci (Weinh). 2023 Oct;10(30):e2303832
pubmed: 37632720
J Am Chem Soc. 2013 Mar 27;135(12):4700-3
pubmed: 23461434
Org Biomol Chem. 2022 Jun 22;20(24):4905-4914
pubmed: 35593095
Carbohydr Res. 2014 Sep 19;396:62-9
pubmed: 25124522
Curr Opin Biotechnol. 2022 Apr;74:247-255
pubmed: 34998108
Chem Rev. 2018 Sep 12;118(17):8359-8413
pubmed: 30141327
Angew Chem Int Ed Engl. 2000 Feb;39(3):531-534
pubmed: 10671247
Chem Rev. 2018 Sep 12;118(17):7886-7930
pubmed: 29553244
Chem Sci. 2018 Aug 31;9(43):8194-8206
pubmed: 30542567
Chemistry. 2008;14(23):7072-81
pubmed: 18601234
Nat Chem. 2019 Feb;11(2):161-169
pubmed: 30532014
J Am Chem Soc. 2019 Sep 18;141(37):14463-14479
pubmed: 31403778
Cell Immunol. 2018 Nov;333:65-79
pubmed: 30107893
Biotechnol Adv. 2023 Oct;67:108169
pubmed: 37207876
Angew Chem Int Ed Engl. 2021 Nov 8;60(46):24686-24693
pubmed: 34520098
J Am Chem Soc. 2009 Nov 25;131(46):16669-71
pubmed: 19886622
J Am Chem Soc. 2016 Mar 16;138(10):3461-8
pubmed: 26926155
Nat Commun. 2018 Aug 17;9(1):3287
pubmed: 30120234
Nat Rev Rheumatol. 2023 Jan;19(1):28-43
pubmed: 36418483
Chemistry. 2016 Dec 23;22(52):18742-18746
pubmed: 27798819
Cancer Res. 1991 Nov 1;51(21):5888-92
pubmed: 1933856
J Biol Chem. 2013 Nov 22;288(47):33598-33610
pubmed: 24108122
Glycobiology. 2014 Jun;24(6):507-17
pubmed: 24658466
Angew Chem Int Ed Engl. 2021 Aug 23;60(35):19287-19296
pubmed: 34124805
Nat Commun. 2018 Jan 17;9(1):258
pubmed: 29343722
Nat Commun. 2016 Apr 12;7:11273
pubmed: 27068492
Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):6954-6959
pubmed: 28630345
Blood. 2011 Jun 16;117(24):6589-99
pubmed: 21536857
MethodsX. 2019 Apr 09;6:773-778
pubmed: 31016140
Nat Rev Nephrol. 2019 Jun;15(6):346-366
pubmed: 30858582
Nature. 2007 Apr 26;446(7139):1046-51
pubmed: 17460666
Nat Chem. 2016 Apr;8(4):338-46
pubmed: 27001729
Biochim Biophys Acta. 1997 Apr 17;1335(1-2):23-32
pubmed: 9133639
Nat Rev Immunol. 2023 Aug;23(8):479-494
pubmed: 36646848
J Clin Invest. 2019 Sep 3;129(9):3492-3498
pubmed: 31478910
J Biol Chem. 1987 Feb 15;262(5):2025-33
pubmed: 3546284
Carbohydr Res. 1986 Aug 15;151:329-35
pubmed: 3094938
Nat Chem. 2019 Mar;11(3):229-236
pubmed: 30792508
J Am Chem Soc. 2020 Nov 18;142(46):19611-19621
pubmed: 33164488
Sci Adv. 2022 Feb 11;8(6):eabm1759
pubmed: 35138894
Science. 2013 Jul 26;341(6144):379-83
pubmed: 23888036
Nat Chem Biol. 2020 Jul;16(7):766-775
pubmed: 32483376
Chem Sci. 2015 Oct 1;6(10):5652-5661
pubmed: 26417422
J Immunol. 1999 Aug 15;163(4):1973-83
pubmed: 10438934
J Immunol. 2016 Feb 15;196(4):1435-41
pubmed: 26851295
J Am Chem Soc. 2011 Oct 19;133(41):16495-502
pubmed: 21859106
J Am Chem Soc. 2011 Nov 23;133(46):18975-91
pubmed: 22004528
Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):3485-3490
pubmed: 28289219
Cancer Immunol Res. 2015 Feb;3(2):173-83
pubmed: 25387893
Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):E1297-306
pubmed: 25733881
J Biochem. 1993 Jun;113(6):692-8
pubmed: 8370666
Nat Commun. 2018 Aug 17;9(1):3288
pubmed: 30120235
Carbohydr Res. 2017 Nov 27;452:122-128
pubmed: 29096185
Org Lett. 2003 Nov 27;5(24):4717-20
pubmed: 14627423
Science. 2001 Dec 7;294(5549):2163-6
pubmed: 11739956
Nat Rev Drug Discov. 2009 Mar;8(3):226-34
pubmed: 19247305
Nat Rev Rheumatol. 2017 Oct;13(10):621-630
pubmed: 28905852