Inhibition of caspase-1-dependent apoptosis suppresses peste des petits ruminants virus replication.
Peste-des-petits-ruminants virus
inflammation
innate immunity
interaction
mononuclear
Journal
Journal of veterinary science
ISSN: 1976-555X
Titre abrégé: J Vet Sci
Pays: Korea (South)
ID NLM: 100964185
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
14
11
2022
revised:
10
05
2023
accepted:
26
05
2023
medline:
1
12
2023
pubmed:
28
8
2023
entrez:
28
8
2023
Statut:
ppublish
Résumé
Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells. PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response. Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4 PPRV stimulated the differentiation of CD4 This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication
Sections du résumé
BACKGROUND
BACKGROUND
Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells.
OBJECTIVES
OBJECTIVE
PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response.
METHODS
METHODS
Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4
RESULTS
RESULTS
PPRV stimulated the differentiation of CD4
CONCLUSIONS
CONCLUSIONS
This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication
Identifiants
pubmed: 37638708
pii: 24.e55
doi: 10.4142/jvs.22288
pmc: PMC10556287
doi:
Substances chimiques
Caspases
EC 3.4.22.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e55Subventions
Organisme : National Key R & D plan
ID : 2022YFD1602203
Pays : China
Organisme : Gansu Provincial Science and Technology Special Mission
ID : 22CX8NA012
Pays : China
Organisme : Special Project on Transformation of Scientific and Technological Achievements
ID : 2022-NK-118
Pays : China
Organisme : Qinghai Provincial Science and Technology Plan
ID : 2021-ZJ-923
Pays : China
Informations de copyright
© 2023 The Korean Society of Veterinary Science.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.