Spectral Flow Cytometry Analysis of Resident Tissue Macrophages.
High-dimensional
Resident tissue macrophages
Spectral flow cytometry
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
29
8
2023
pubmed:
28
8
2023
entrez:
28
8
2023
Statut:
ppublish
Résumé
Spectral flow cytometry improves flow cytometry panels by resolving the full emission spectra of individual fluorophores, allowing greater flexibility to incorporate more fluorochromes when designing multicolor panels. Additionally, the spectral approach captures the autofluorescence of a sample or cell population (e.g., macrophages, which are highly autofluorescent) that can be considered during unmixing for improved downstream analyses. As the increased complexity of macrophage heterogeneity unravels in the scientific community, it is crucial to obtain high-dimensional data at the single-cell level to resolve these populations.
Identifiants
pubmed: 37639129
doi: 10.1007/978-1-0716-3437-0_18
doi:
Substances chimiques
Fluorescent Dyes
0
Ionophores
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
269-280Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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pubmed: 32553181
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doi: 10.1126/science.aau0964
pubmed: 30872492
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pubmed: 26441984
pmcid: 4585205
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pmcid: 3720686
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