High-resolution crystal structure of the Mu8.1 conotoxin from Conus mucronatus.

Mu8.1 conotoxins hydrogen bonding toxins zinc binding

Journal

Acta crystallographica. Section F, Structural biology communications
ISSN: 2053-230X
Titre abrégé: Acta Crystallogr F Struct Biol Commun
Pays: United States
ID NLM: 101620319

Informations de publication

Date de publication:
01 Sep 2023
Historique:
received: 18 06 2023
accepted: 10 08 2023
medline: 6 9 2023
pubmed: 29 8 2023
entrez: 29 8 2023
Statut: ppublish

Résumé

Marine cone snails produce a wealth of peptide toxins (conotoxins) that bind their molecular targets with high selectivity and potency. Therefore, conotoxins constitute valuable biomolecular tools with a variety of biomedical purposes. The Mu8.1 conotoxin from Conus mucronatus is the founding member of the newly identified saposin-like conotoxin class of conotoxins and has been shown to target Cav2.3, a voltage-gated calcium channel. Two crystal structures have recently been determined of Mu8.1 at 2.3 and 2.1 Å resolution. Here, a high-resolution crystal structure of Mu8.1 was determined at 1.67 Å resolution in the high-symmetry space group I4

Identifiants

pubmed: 37642664
pii: S2053230X23007070
doi: 10.1107/S2053230X23007070
pmc: PMC10478764
doi:

Substances chimiques

Conotoxins 0
Lysine K3Z4F929H6
Water 059QF0KO0R

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

240-246

Subventions

Organisme : Teknologi og Produktion, Det Frie Forskningsråd
ID : 7017-00288

Informations de copyright

open access.

Références

Front Pharmacol. 2021 May 13;12:655981
pubmed: 34054536
Protein Sci. 1998 Aug;7(8):1700-16
pubmed: 10082367
Arch Biochem Biophys. 2003 Mar 1;411(1):112-20
pubmed: 12590929
Nat Chem. 2017 Dec;9(12):1181-1190
pubmed: 29168484
Bioessays. 2016 Jun;38(6):539-48
pubmed: 27166747
J Gen Physiol. 2012 Mar;139(3):219-34
pubmed: 22371363
J Synchrotron Radiat. 2020 Sep 1;27(Pt 5):1415-1429
pubmed: 32876619
Expert Opin Drug Discov. 2021 Oct;16(10):1163-1173
pubmed: 33914674
Nat Struct Mol Biol. 2016 Oct;23(10):916-920
pubmed: 27617429
J Phys Chem B. 2009 Mar 5;113(9):2952-60
pubmed: 19708219
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
PLoS Biol. 2023 Aug 3;21(8):e3002217
pubmed: 37535677
Proc Natl Acad Sci U S A. 2015 Feb 10;112(6):1743-8
pubmed: 25605914
J Chem Theory Comput. 2011 Feb 8;7(2):525-37
pubmed: 26596171
Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5260-5
pubmed: 21389271
J Biol Chem. 2019 May 31;294(22):8745-8759
pubmed: 30975904
Science. 2014 Aug 29;345(6200):1021-6
pubmed: 25103405
J Biol Chem. 1999 Dec 31;274(53):37705-8
pubmed: 10608828
Nat Protoc. 2014 Jan;9(1):156-70
pubmed: 24356774
Elife. 2019 Feb 12;8:
pubmed: 30747102
Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4085-90
pubmed: 24591597
Cell. 1981 Feb;23(2):411-22
pubmed: 7471207
Curr Biol. 2009 Jun 9;19(11):900-8
pubmed: 19481459
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Acta Crystallogr D Struct Biol. 2016 Sep;72(Pt 9):1036-48
pubmed: 27599736
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
J Appl Crystallogr. 2009 Dec 1;42(Pt 6):1035-1042
pubmed: 22477774
J Chem Theory Comput. 2011 Jul 12;7(7):2284-95
pubmed: 26606496
J Gen Physiol. 2022 May 2;154(5):
pubmed: 35377397

Auteurs

Emilie Müller (E)

Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.

Celeste Menuet Hackney (CM)

Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark.

Lars Ellgaard (L)

Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark.

Jens Preben Morth (JP)

Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.

Articles similaires

Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH