3D-Printed Osteoinductive Polymeric Scaffolds with Optimized Architecture to Repair a Sheep Metatarsal Critical-Size Bone Defect.


Journal

Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613

Informations de publication

Date de publication:
12 2023
Historique:
revised: 10 08 2023
received: 26 05 2023
medline: 5 12 2023
pubmed: 1 9 2023
entrez: 1 9 2023
Statut: ppublish

Résumé

The reconstruction of critical-size bone defects in long bones remains a challenge for clinicians. A new osteoinductive medical device is developed here for long bone repair by combining a 3D-printed architectured cylindrical scaffold made of clinical-grade polylactic acid (PLA) with a polyelectrolyte film coating delivering the osteogenic bone morphogenetic protein 2 (BMP-2). This film-coated scaffold is used to repair a sheep metatarsal 25-mm long critical-size bone defect. In vitro and in vivo biocompatibility of the film-coated PLA material is proved according to ISO standards. Scaffold geometry is found to influence BMP-2 incorporation. Bone regeneration is followed using X-ray scans, µCT scans, and histology. It is shown that scaffold internal geometry, notably pore shape, influenced bone regeneration, which is homogenous longitudinally. Scaffolds with cubic pores of ≈870 µm and a low BMP-2 dose of ≈120 µg cm

Identifiants

pubmed: 37655491
doi: 10.1002/adhm.202301692
doi:

Substances chimiques

Polyesters 0
Polymers 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2301692

Subventions

Organisme : H2020 European Research Council
ID : GA259370
Organisme : H2020 European Research Council
ID : GA692924
Organisme : H2020 European Research Council
ID : GA790435
Organisme : Union des Blessés de la Face et de la Tête
ID : 33-2022
Organisme : Agence Nationale de la Recherche
ID : ANR-18-CE17-0016
Organisme : Infrastructure d'avenir en Biologie Santé
ID : ANR-11-INBS-0006

Informations de copyright

© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.

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Auteurs

Charlotte Garot (C)

CNRS EMR 5000 Biomimetism and Regenerative Medicine (BRM), INSERM U1292 Biosanté, CEA, Université Grenoble Alpes, 17 avenue des Martyrs, Grenoble, F-38054, France.

Sarah Schoffit (S)

Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, F-94704, France.
CNRS, INSERM, ENVA, B3OA, Université Paris Cité, Paris, F-75010, France.

Cécile Monfoulet (C)

INSERM, Institut Bergonié, University of Bordeaux, CIC 1401, Bordeaux, F-33000, France.
CIC-IT, INSERM, Institut Bergonié, CHU de Bordeaux, CIC 1401, Bordeaux, F-33000, France.

Paul Machillot (P)

CNRS EMR 5000 Biomimetism and Regenerative Medicine (BRM), INSERM U1292 Biosanté, CEA, Université Grenoble Alpes, 17 avenue des Martyrs, Grenoble, F-38054, France.

Claire Deroy (C)

INSERM, Institut Bergonié, University of Bordeaux, CIC 1401, Bordeaux, F-33000, France.
CIC-IT, INSERM, Institut Bergonié, CHU de Bordeaux, CIC 1401, Bordeaux, F-33000, France.

Samantha Roques (S)

INSERM, Institut Bergonié, University of Bordeaux, CIC 1401, Bordeaux, F-33000, France.
CIC-IT, INSERM, Institut Bergonié, CHU de Bordeaux, CIC 1401, Bordeaux, F-33000, France.

Julie Vial (J)

Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, F-94704, France.
CNRS, INSERM, ENVA, B3OA, Université Paris Cité, Paris, F-75010, France.

Julien Vollaire (J)

INSERM U1209, Institute of Advanced Biosciences, Grenoble, F-38000, France.
Institute of Advanced Biosciences, Université Grenoble Alpes, Grenoble, F-38000, France.

Martine Renard (M)

INSERM, Institut Bergonié, University of Bordeaux, CIC 1401, Bordeaux, F-33000, France.
CIC-IT, INSERM, Institut Bergonié, CHU de Bordeaux, CIC 1401, Bordeaux, F-33000, France.

Hasan Ghanem (H)

CNRS, INSERM, ENVA, B3OA, Université Paris Cité, Paris, F-75010, France.

Hanane El-Hafci (H)

CNRS, INSERM, ENVA, B3OA, Université Paris Cité, Paris, F-75010, France.

Adeline Decambron (A)

Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, F-94704, France.
CNRS, INSERM, ENVA, B3OA, Université Paris Cité, Paris, F-75010, France.

Véronique Josserand (V)

INSERM U1209, Institute of Advanced Biosciences, Grenoble, F-38000, France.
Institute of Advanced Biosciences, Université Grenoble Alpes, Grenoble, F-38000, France.

Laurence Bordenave (L)

INSERM, Institut Bergonié, University of Bordeaux, CIC 1401, Bordeaux, F-33000, France.
CIC-IT, INSERM, Institut Bergonié, CHU de Bordeaux, CIC 1401, Bordeaux, F-33000, France.

Georges Bettega (G)

INSERM U1209, Institute of Advanced Biosciences, Grenoble, F-38000, France.
Service de Chirurgie Maxillo-Faciale, Centre Hospitalier Annecy Genevois, 1 avenue de l'hôpital, Epagny Metz-Tessy, F-74370, France.

Marlène Durand (M)

INSERM, Institut Bergonié, University of Bordeaux, CIC 1401, Bordeaux, F-33000, France.
CIC-IT, INSERM, Institut Bergonié, CHU de Bordeaux, CIC 1401, Bordeaux, F-33000, France.

Mathieu Manassero (M)

Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, F-94704, France.
CNRS, INSERM, ENVA, B3OA, Université Paris Cité, Paris, F-75010, France.

Véronique Viateau (V)

Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, F-94704, France.
CNRS, INSERM, ENVA, B3OA, Université Paris Cité, Paris, F-75010, France.

Delphine Logeart-Avramoglou (D)

CNRS, INSERM, ENVA, B3OA, Université Paris Cité, Paris, F-75010, France.

Catherine Picart (C)

CNRS EMR 5000 Biomimetism and Regenerative Medicine (BRM), INSERM U1292 Biosanté, CEA, Université Grenoble Alpes, 17 avenue des Martyrs, Grenoble, F-38054, France.
Institut Universitaire de France (IUF), 1 rue Descartes, Paris CEDEX 05, 75231, France.

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