A Versatile and Efficient Method to Isolate DNA-Polymer Conjugates.
Journal
ACS macro letters
ISSN: 2161-1653
Titre abrégé: ACS Macro Lett
Pays: United States
ID NLM: 101574672
Informations de publication
Date de publication:
19 09 2023
19 09 2023
Historique:
medline:
20
9
2023
pubmed:
1
9
2023
entrez:
1
9
2023
Statut:
ppublish
Résumé
We present a facile and adaptable method to purify and isolate DNA-polymer conjugates from different uncharged homo, random, or block copolymer families. Anion exchange chromatography is used to separate the reaction solution and retrieve the excess unreacted polymer and oligonucleotide. The stationary phase has a high efficiency (25 nmol of DNA per run), facilitating the purification of large batches without compromising the peak shape and resolution. To demonstrate the versatility of this method, different types of polymers, including acrylates, methacrylates, and acrylamides containing hydrophilic and hydrophobic blocks, were purified with high yields. Additionally, DNA-polymer conjugates with various DNA block lengths were also successfully purified, further highlighting the broad applicability of this method.
Identifiants
pubmed: 37656875
doi: 10.1021/acsmacrolett.3c00371
pmc: PMC10515633
doi:
Substances chimiques
DNA
9007-49-2
Oligonucleotides
0
Acrylamides
0
Acrylates
0
Polymers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1257-1263Références
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