Chimeric versus isolated proteins: Biochemical characterization of the NADP

Mixed Function Oxygenases / chemistry NADP / metabolism Formate Dehydrogenases / chemistry NADPH Dehydrogenase Pseudomonas / genetics
Fused enzymes Protein conformation Protein promiscuity

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
31 Dec 2023
Historique:
received: 16 05 2023
revised: 04 08 2023
accepted: 29 08 2023
medline: 27 11 2023
pubmed: 2 9 2023
entrez: 1 9 2023
Statut: ppublish

Résumé

The expression of multifunctional proteins can facilitate the setup of a biotechnology process that requires multiple functions absolved by different proteins. Herein the functional and conformational characterization of a formate dehydrogenase-monooxygenase chimera enzyme is presented. The fused enzyme (FDH-PAMO) was prepared by linking the C-terminus of the mutant NADP

Identifiants

DOI: 10.1016/j.ijbiomac.2023.126637 PMID: 37657580
pubmed: 37657580
pii: S0141-8130(23)03533-X
doi: 10.1016/j.ijbiomac.2023.126637
pii:
doi:

Substances chimiques

Mixed Function Oxygenases EC 1.-
NADP 53-59-8
Formate Dehydrogenases EC 1.17.1.9
NADPH Dehydrogenase EC 1.6.99.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

126637

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Francesco Secundo reports financial support was provided by European Union. Anastasia A. Pometun reports financial support was provided by Russian Foundation for Basic Research and Moscow Government. S. Yu Kleymenov reports financial support was provided by Ministry of Science and Higher Education of Russia.

Auteurs

Elia Lio (E)

Istituto di Scienze e Tecnologie Chimiche, CNR, Via Mario Bianco 9, 20131 Milan, Italy.

Pavel Parshin (P)

Chemistry Faculty, M.V. Lomonosov Moscow State University, Leninskie Gory 1-3, 119991 Moscow, Russian Federation.

Erica D'Oronzo (E)

Istituto di Scienze e Tecnologie Chimiche, CNR, Via Mario Bianco 9, 20131 Milan, Italy.

Stefano Plebani (S)

Istituto di Scienze e Tecnologie Chimiche, CNR, Via Mario Bianco 9, 20131 Milan, Italy.

Anastasia A Pometun (AA)

Chemistry Faculty, M.V. Lomonosov Moscow State University, Leninskie Gory 1-3, 119991 Moscow, Russian Federation; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, bld. 33-2 Leninsky Ave., Moscow 119071, Russian Federation.

S Yu Kleymenov (SY)

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, bld. 33-2 Leninsky Ave., Moscow 119071, Russian Federation; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Vavilova 26, Moscow 119334, Russian Federation.

Vladimir I Tishkov (VI)

Chemistry Faculty, M.V. Lomonosov Moscow State University, Leninskie Gory 1-3, 119991 Moscow, Russian Federation; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, bld. 33-2 Leninsky Ave., Moscow 119071, Russian Federation.

Francesco Secundo (F)

Istituto di Scienze e Tecnologie Chimiche, CNR, Via Mario Bianco 9, 20131 Milan, Italy. Electronic address: francesco.secundo@scitec.cnr.it.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxygénases › Mixed function oxygenases › Chimeric versus isolated proteins: Biochemical...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › NADP › Chimeric versus isolated proteins: Biochemical...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxidoreductases acting on aldehyde or oxo group donors › Formate dehydrogenases › Chimeric versus isolated proteins: Biochemical...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › NADH, NADPH oxidoreductases › NADPH dehydrogenase › Chimeric versus isolated proteins: Biochemical...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pseudomonadaceae › Pseudomonas › Chimeric versus isolated proteins: Biochemical...