The regulation of simulated artificial oro-gastrointestinal transit stress on the adhesion of Lactobacillus plantarum S7.
Adhesion ability
Lactobacillus plantarum S7
Regulation
Simulated oro-gastrointestinal transit stress
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
02 Sep 2023
02 Sep 2023
Historique:
received:
08
04
2023
accepted:
09
08
2023
medline:
4
9
2023
pubmed:
3
9
2023
entrez:
2
9
2023
Statut:
epublish
Résumé
Oro-gastrointestinal stress in the digestive tract is the main stress to which orally administered probiotics are exposed. The regulation of oro-gastrointestinal transit (OGT) stress on the adhesion and survival of probiotics under continuous exposure to simulated salivary-gastric juice-intestinal juice was researched in this study. Lactobacillus plantarum S7 had a higher survival rate after exposure to simulated OGT1 (containing 0.15% bile salt) stress and OGT2 (containing 0.30% bile salt) stress. The adhesion ability of L. plantarum S7 was significantly increased by OGT1 stress (P < 0.05) but was not changed significantly by OGT2 stress (P > 0.05), and this trend was also observed in terms of the thickness of the surface material of L. plantarum S7 cells. The expression of surface proteins of L. plantarum S7, such as the 30 S ribosomal proteins, mucus-binding protein and S-layer protein, was significantly downregulated by OGT stress (P < 0.05); meanwhile, the expression of moonlight proteins, such as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycorate kinase (PGK), beta-phosphoglucomutase (PGM1), GroEL and glucose-6-phosphate isomerase (PGI), was significantly upregulated (P < 0.05). However, the upregulation of GAPDH, PGK, PGM1 and PGI mediated by OGT1 stress was greater than those mediated by OGT2 stress. The quorum sensing pathway of L. plantarum S7 was changed significantly by OGT stress compared with no OGT stress cells (P < 0.05), and the expression of Luxs in the pathway was significantly upregulated by OGT1 stress (P < 0.05). The ABC transportation pathway was significantly altered by OGT1 stress (P < 0.05), of which the expression of the peptide ABC transporter substrate-binding protein and energy-coupling factor transporter ATP-binding protein EcfA was significantly upregulated by OGT stress (P < 0.05). The glycolide metabolism pathway was significantly altered by OGT1 stress compared with that in response to OGT2 stress (P < 0.05). L. plantarum S7 had a strong ability to resist OGT stress, which was regulated by the proteins and pathways related to OGT stress. The adhesion ability of L. plantarum S7 was enhanced after continuous exposure to OGT1 stress, making it a potential probiotic with a promising future for application.
Sections du résumé
BACKGROUND
BACKGROUND
Oro-gastrointestinal stress in the digestive tract is the main stress to which orally administered probiotics are exposed. The regulation of oro-gastrointestinal transit (OGT) stress on the adhesion and survival of probiotics under continuous exposure to simulated salivary-gastric juice-intestinal juice was researched in this study.
RESULTS
RESULTS
Lactobacillus plantarum S7 had a higher survival rate after exposure to simulated OGT1 (containing 0.15% bile salt) stress and OGT2 (containing 0.30% bile salt) stress. The adhesion ability of L. plantarum S7 was significantly increased by OGT1 stress (P < 0.05) but was not changed significantly by OGT2 stress (P > 0.05), and this trend was also observed in terms of the thickness of the surface material of L. plantarum S7 cells. The expression of surface proteins of L. plantarum S7, such as the 30 S ribosomal proteins, mucus-binding protein and S-layer protein, was significantly downregulated by OGT stress (P < 0.05); meanwhile, the expression of moonlight proteins, such as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycorate kinase (PGK), beta-phosphoglucomutase (PGM1), GroEL and glucose-6-phosphate isomerase (PGI), was significantly upregulated (P < 0.05). However, the upregulation of GAPDH, PGK, PGM1 and PGI mediated by OGT1 stress was greater than those mediated by OGT2 stress. The quorum sensing pathway of L. plantarum S7 was changed significantly by OGT stress compared with no OGT stress cells (P < 0.05), and the expression of Luxs in the pathway was significantly upregulated by OGT1 stress (P < 0.05). The ABC transportation pathway was significantly altered by OGT1 stress (P < 0.05), of which the expression of the peptide ABC transporter substrate-binding protein and energy-coupling factor transporter ATP-binding protein EcfA was significantly upregulated by OGT stress (P < 0.05). The glycolide metabolism pathway was significantly altered by OGT1 stress compared with that in response to OGT2 stress (P < 0.05).
CONCLUSION
CONCLUSIONS
L. plantarum S7 had a strong ability to resist OGT stress, which was regulated by the proteins and pathways related to OGT stress. The adhesion ability of L. plantarum S7 was enhanced after continuous exposure to OGT1 stress, making it a potential probiotic with a promising future for application.
Identifiants
pubmed: 37660047
doi: 10.1186/s12934-023-02174-3
pii: 10.1186/s12934-023-02174-3
pmc: PMC10474686
doi:
Substances chimiques
Bile Acids and Salts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
170Subventions
Organisme : National key research and development program of the fourteen of China
ID : 2022YFD2101503-4
Organisme : National Natural Science Foundation of China
ID : 32272362
Organisme : Natural Science Foundation of Jiangsu Province
ID : BK20211325
Organisme : Key Laboratory of Probiotics and Dairy Deep Processing of Yangzhou
ID : YZ2020265
Informations de copyright
© 2023. BioMed Central Ltd., part of Springer Nature.
Références
Appl Environ Microbiol. 2002 Feb;68(2):784-90
pubmed: 11823219
Appl Environ Microbiol. 2008 Aug;74(15):4711-8
pubmed: 18539797
Theriogenology. 2020 Sep 15;154:181-189
pubmed: 32622198
Microbiol Res. 2013 Dec 14;168(10):639-45
pubmed: 23890721
Appl Environ Microbiol. 1999 Mar;65(3):1071-7
pubmed: 10049865
J Mol Microbiol Biotechnol. 2010;18(4):206-14
pubmed: 20559014
FEMS Microbiol Rev. 2005 Sep;29(4):625-51
pubmed: 16102595
Nucleic Acids Res. 2012 Jan;40(Database issue):D109-14
pubmed: 22080510
J Bacteriol. 2007 Aug;189(16):5929-36
pubmed: 17557824
Probiotics Antimicrob Proteins. 2022 Nov 14;:
pubmed: 36376613
Biotechnol Lett. 2012 Aug;34(8):1511-8
pubmed: 22526425
Nat Methods. 2009 May;6(5):359-62
pubmed: 19377485
J Mol Biol. 2001 Jan 12;305(2):245-57
pubmed: 11124903
Int J Food Microbiol. 2010 Aug 15;142(1-2):132-41
pubmed: 20621375
World J Microbiol Biotechnol. 2019 Oct 1;35(10):156
pubmed: 31576430
Clin Nutr. 2020 May;39(5):1315-1323
pubmed: 31174942
Biochim Biophys Acta. 2010 Apr;1803(4):520-5
pubmed: 20144902
J Dairy Sci. 2015 Oct;98(10):6759-66
pubmed: 26254535
J Appl Microbiol. 2008 Jun;104(6):1667-74
pubmed: 18194256
Mediators Inflamm. 2013;2013:237921
pubmed: 23576850
Mol Cell Proteomics. 2014 Oct;13(10):2593-603
pubmed: 24997997
Trends Microbiol. 2013 Dec;21(12):652-9
pubmed: 24156876
Appl Microbiol Biotechnol. 2022 Jan;106(2):755-771
pubmed: 35015143
Biotechnol Adv. 2001 Dec;19(8):597-625
pubmed: 14550013
Microbiol Spectr. 2022 Aug 31;10(4):e0061022
pubmed: 35700135
Gut Microbes. 2010 Jul;1(4):254-268
pubmed: 21327032
Biotechnol Adv. 2011 Jan-Feb;29(1):54-66
pubmed: 20807563
J Bacteriol. 2012 May;194(10):2509-19
pubmed: 22389474
J Appl Microbiol. 2010 Sep;109(3):927-35
pubmed: 20408914
Appl Environ Microbiol. 2000 Aug;66(8):3519-27
pubmed: 10919816
J Appl Microbiol. 2009 Jul;107(1):269-79
pubmed: 19302300
J Dairy Sci. 2021 Feb;104(2):1474-1483
pubmed: 33246623
J Appl Microbiol. 2014 Jan;116(1):134-44
pubmed: 24016102
Appl Environ Microbiol. 1989 Jun;55(6):1549-54
pubmed: 16347948
Colloids Surf B Biointerfaces. 2005 Mar 10;41(1):33-41
pubmed: 15698754
Infect Immun. 2006 Jan;74(1):425-34
pubmed: 16368998
Biology (Basel). 2014 Mar 10;3(1):178-204
pubmed: 24833341
Mol Microbiol. 2007 Mar;63(5):1285-95
pubmed: 17302811
Front Microbiol. 2019 Apr 24;10:841
pubmed: 31068918
Curr Microbiol. 2020 Dec;77(12):3831-3841
pubmed: 33079206
Curr Opin Microbiol. 2012 Jun;15(3):390-6
pubmed: 22538051
Int J Food Microbiol. 2009 Nov 15;135(3):295-302
pubmed: 19748697
PLoS One. 2020 Oct 29;15(10):e0241310
pubmed: 33119648
Infect Immun. 2000 Sep;68(9):4839-49
pubmed: 10948095
Probiotics Antimicrob Proteins. 2019 Jun;11(2):382-396
pubmed: 29542032
J Proteome Res. 2013 Jan 4;12(1):432-43
pubmed: 23181408
Proteomics. 2015 Jul;15(13):2244-57
pubmed: 25728239
Proteomes. 2021 Feb 10;9(1):
pubmed: 33578796
Microorganisms. 2022 Feb 08;10(2):
pubmed: 35208844
J Biosci Bioeng. 2021 Feb;131(2):153-160
pubmed: 33077360
Proteomics. 2004 Jan;4(1):106-22
pubmed: 14730676
Biochem Soc Trans. 1988 Apr;16(2):87-9
pubmed: 3131165
Appl Environ Microbiol. 2008 Mar;74(6):1812-9
pubmed: 18245259
Langmuir. 2019 Jan 15;35(2):529-537
pubmed: 30567428
Microb Pathog. 2007 Aug-Sep;43(2-3):78-87
pubmed: 17524609
J Proteome Res. 2021 May 7;20(5):2447-2457
pubmed: 33705137
Microbiol Res. 2013 Jul 19;168(6):351-359
pubmed: 23414698
DNA Seq. 2003 Apr;14(2):141-5
pubmed: 12825356
BMC Microbiol. 2013 Sep 17;13:210
pubmed: 24044741