Application of a simplified PCR-SSP method to detect A4GALT*01 and A4GALT*02 typing among Thai blood donors.
A4GALT
P1PK
PCR-SSP
Red blood cell genotyping
Thai blood donors
Journal
Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine
ISSN: 1953-8022
Titre abrégé: Transfus Clin Biol
Pays: France
ID NLM: 9423846
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
04
07
2023
revised:
30
08
2023
accepted:
30
08
2023
medline:
30
10
2023
pubmed:
7
9
2023
entrez:
6
9
2023
Statut:
ppublish
Résumé
An intronic A4GALT single nucleotide variant, rs5751348:G>T, P The conventional test tube technique was used to detect the P1 antigen in 222 blood samples from Thai blood donors at Thammasat University Hospital. A PCR-SSP method was optimized and validated for reproducibility and specificity to identify these alleles and was subsequently tested on 1,840 DNA samples of unknown phenotypes obtained from central, northern and southern Thais. In addition, allele frequencies of central Thais were compared with those of other populations. In the tested cohort (n = 222), P Our study has successfully developed a simple, precise, and reliable method to genotype A4GALT*01 and A4GALT*02 using inhouse developed PCR-SSP for predicting P
Identifiants
pubmed: 37673392
pii: S1246-7820(23)00101-5
doi: 10.1016/j.tracli.2023.08.007
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
436-442Informations de copyright
Copyright © 2023 Société française de transfusion sanguine (SFTS). Published by Elsevier Masson SAS. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.