Structural Insight into the Catalytic Mechanisms of an L-Sorbosone Dehydrogenase.
2-keto-L-gulonic acid
Gluconobacter oxydans
Vitamin C
redox control
substrate pocket
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
10 2023
10 2023
Historique:
revised:
29
07
2023
received:
26
03
2023
medline:
30
10
2023
pubmed:
8
9
2023
entrez:
7
9
2023
Statut:
ppublish
Résumé
L-Sorbosone dehydrogenase (SNDH) is a key enzyme involved in the biosynthesis of 2-keto-L-gulonic acid , which is a direct precursor for the industrial scale production of vitamin C. Elucidating the structure and the catalytic mechanism is essential for improving SNDH performance. By solving the crystal structures of SNDH from Gluconobacter oxydans WSH-004, a reversible disulfide bond between Cys295 and the catalytic Cys296 residues is discovered. It allowed SNDH to switch between oxidation and reduction states, resulting in opening or closing the substrate pocket. Moreover, the Cys296 is found to affect the NADP
Identifiants
pubmed: 37679059
doi: 10.1002/advs.202301955
pmc: PMC10602560
doi:
Substances chimiques
L-sorbosone dehydrogenase
EC 1.2.99.-
Aldehyde Oxidoreductases
EC 1.2.-
Ascorbic Acid
PQ6CK8PD0R
Vitamins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2301955Subventions
Organisme : National Natural Science Foundation of China
ID : 31830068
Organisme : Foundation for Innovative Research Groups of the National Natural Science Foundation of China
ID : 32021005
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
Références
Protein Sci. 2021 Aug;30(8):1714-1722
pubmed: 34048122
J Phys Chem B. 2020 Nov 5;124(44):9840-9851
pubmed: 33111518
Chem Biol Interact. 2003 Feb 1;143-144:85-91
pubmed: 12604192
J Biotechnol. 2019 Aug 10;301:24-34
pubmed: 31136757
Commun Biol. 2022 Apr 13;5(1):354
pubmed: 35418200
Acta Crystallogr D Biol Crystallogr. 2015 May;71(Pt 5):1159-75
pubmed: 25945581
J Biol Chem. 2011 Jul 1;286(26):23357-67
pubmed: 21540484
Foods. 2021 Jan 19;10(1):
pubmed: 33478115
Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):E4724-E4733
pubmed: 28559343
J Comput Chem. 2004 Jul 15;25(9):1157-74
pubmed: 15116359
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
Curr Protoc Bioinformatics. 2016 Jun 20;54:5.6.1-5.6.37
pubmed: 27322406
Structure. 1997 May 15;5(5):701-11
pubmed: 9195888
Nucleic Acids Res. 2021 Jan 8;49(D1):D1388-D1395
pubmed: 33151290
Nat Struct Biol. 1997 Apr;4(4):317-26
pubmed: 9095201
J Inherit Metab Dis. 2020 May;43(3):635-644
pubmed: 31652343
Nat Commun. 2019 Oct 4;10(1):4527
pubmed: 31586059
Biomolecules. 2019 Aug 16;9(8):
pubmed: 31426424
Chem Biol Interact. 2003 Feb 1;143-144:23-8
pubmed: 12604185
J Mol Graph. 1996 Feb;14(1):33-8, 27-8
pubmed: 8744570
J Comput Chem. 2003 Oct;24(13):1549-62
pubmed: 12925999
Acta Biochim Pol. 2018;65(1):125-132
pubmed: 29518171
Nat Commun. 2019 Jul 19;10(1):3198
pubmed: 31324776
Phys Rev B Condens Matter. 1988 Jan 15;37(2):785-789
pubmed: 9944570
J Chem Theory Comput. 2015 Aug 11;11(8):3696-713
pubmed: 26574453
J Mol Biol. 2019 Feb 1;431(3):576-592
pubmed: 30580036
J Biol Chem. 1997 Jul 25;272(30):18817-22
pubmed: 9228056
Methods Enzymol. 1997;276:307-26
pubmed: 27754618
PLoS Comput Biol. 2012;8(10):e1002708
pubmed: 23093919
Nat Chem Biol. 2015 Feb;11(2):156-63
pubmed: 25580853
Nat Commun. 2020 Mar 18;11(1):1426
pubmed: 32188856
Microorganisms. 2022 Jul 18;10(7):
pubmed: 35889163
Front Microbiol. 2015 Sep 16;6:957
pubmed: 26441891
J Chem Phys. 2010 Apr 21;132(15):154104
pubmed: 20423165
Front Bioeng Biotechnol. 2020 Mar 17;8:194
pubmed: 32258011
Biotechnol Adv. 2022 Jan-Feb;54:107863
pubmed: 34793881
Alcohol. 1997 Mar-Apr;14(2):181-9
pubmed: 9085720
J Comput Chem. 2011 May;32(7):1456-65
pubmed: 21370243
Chem Biol Interact. 2011 May 30;191(1-3):269-77
pubmed: 21338592
Nat Chem Biol. 2022 Oct;18(10):1065-1075
pubmed: 35788181
Chemistry. 2010 May 3;16(17):5097-101
pubmed: 20349464
FASEB J. 2021 Feb;35(2):e21219
pubmed: 33236467
EMBO J. 2009 Apr 8;28(7):959-68
pubmed: 19300440
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
J Mol Biol. 2020 Mar 27;432(7):2186-2203
pubmed: 32087198
Adv Sci (Weinh). 2023 Oct;10(30):e2301955
pubmed: 37679059
Sci Rep. 2018 Sep 6;8(1):13327
pubmed: 30190503
Biotechnol Adv. 2018 Nov 15;36(7):1882-1899
pubmed: 30031778
Front Bioeng Biotechnol. 2019 Dec 13;7:385
pubmed: 31921801
Science. 1985 Oct 11;230(4722):144-9
pubmed: 17842676
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Chem Biol Interact. 2001 Jan 30;130-132(1-3):173-9
pubmed: 11306041
Protein Sci. 2016 Nov;25(11):2045-2053
pubmed: 27571338
Protein Eng Des Sel. 2017 Mar 1;30(3):271-278
pubmed: 28338942
Expert Opin Drug Metab Toxicol. 2008 Jun;4(6):697-720
pubmed: 18611112
Front Microbiol. 2021 Feb 25;12:643472
pubmed: 33717042
Phys Chem Chem Phys. 2005 Sep 21;7(18):3297-305
pubmed: 16240044
Microb Cell Fact. 2014 Oct 18;13:146
pubmed: 25323199
Bioresour Technol. 2022 Feb;346:126662
pubmed: 34999190
Oral Dis. 2016 Sep;22(6):463-93
pubmed: 26808119
Metab Eng. 2014 Jul;24:30-7
pubmed: 24792618
J Phys Chem B. 2009 May 7;113(18):6378-96
pubmed: 19366259
Front Bioeng Biotechnol. 2021 Apr 09;9:673844
pubmed: 33898410
J Mol Biol. 2009 Jan 16;385(2):542-57
pubmed: 19013472
J Chem Theory Comput. 2011 Jul 12;7(7):2284-95
pubmed: 26606496
Biochemistry. 2005 Jun 7;44(22):8022-9
pubmed: 15924421
Bioresour Technol. 2020 Dec;318:124069
pubmed: 32916460
Biosci Rep. 2019 Apr 26;39(4):
pubmed: 30914451