Anti-fungal bioactive terpenoids in the bioenergy crop switchgrass (Panicum virgatum) may contribute to ecotype-specific microbiome composition.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
07 09 2023
07 09 2023
Historique:
received:
21
04
2023
accepted:
25
08
2023
medline:
11
9
2023
pubmed:
8
9
2023
entrez:
7
9
2023
Statut:
epublish
Résumé
Plant derived bioactive small molecules have attracted attention of scientists across fundamental and applied scientific disciplines. We seek to understand the influence of these phytochemicals on rhizosphere and root-associated fungi. We hypothesize that - consistent with accumulating evidence that switchgrass genotype impacts microbiome assembly - differential terpenoid accumulation contributes to switchgrass ecotype-specific microbiome composition. An initial in vitro Petri plate-based disc diffusion screen of 18 switchgrass root derived fungal isolates revealed differential responses to upland- and lowland-isolated metabolites. To identify specific fungal growth-modulating metabolites, we tested fractions from root extracts on three ecologically important fungal isolates - Linnemania elongata, Trichoderma sp. and Fusarium sp. Saponins and diterpenoids were identified as the most prominent antifungal metabolites. Finally, analysis of liquid chromatography-purified terpenoids revealed fungal inhibition structure - activity relationships (SAR). Saponin antifungal activity was primarily determined by the number of sugar moieties - saponins glycosylated at a single core position were inhibitory whereas saponins glycosylated at two core positions were inactive. Saponin core hydroxylation and acetylation were also associated with reduced activity. Diterpenoid activity required the presence of an intact furan ring for strong fungal growth inhibition. These results inform future breeding and biotechnology strategies for crop protection with reduced pesticide application.
Identifiants
pubmed: 37679469
doi: 10.1038/s42003-023-05290-3
pii: 10.1038/s42003-023-05290-3
pmc: PMC10485007
doi:
Substances chimiques
Terpenes
0
Antifungal Agents
0
Banques de données
Dryad
['10.5061/dryad.stqjq2c7x']
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
917Informations de copyright
© 2023. Springer Nature Limited.
Références
Planta. 1988 Dec;173(4):474-81
pubmed: 24226684
Annu Rev Plant Biol. 2021 Jun 17;72:185-216
pubmed: 33848429
New Phytol. 2018 Nov;220(3):692-702
pubmed: 27604856
Sci Rep. 2021 Jan 11;11(1):333
pubmed: 33431904
Nat Rev Microbiol. 2017 Oct;15(10):579-590
pubmed: 28824177
Anal Chem. 2008 Jan 1;80(1):115-22
pubmed: 18027910
Nat Biotechnol. 2021 Apr;39(4):462-471
pubmed: 33230292
Nat Rev Microbiol. 2020 Nov;18(11):607-621
pubmed: 32788714
Biophys J. 1999 Jan;76(1 Pt 1):281-90
pubmed: 9876141
Nat Food. 2021 Nov;2(11):873-885
pubmed: 37117503
Phytochemistry. 2011 Apr;72(6):435-57
pubmed: 21333312
J Mol Model. 2010 Jan;16(1):107-18
pubmed: 19513766
J Microbiol Methods. 2015 Apr;111:64-71
pubmed: 25655778
Proc Natl Acad Sci U S A. 2019 Jun 25;116(26):12933-12941
pubmed: 31182579
Plant Physiol. 2018 Apr;176(4):2677-2690
pubmed: 29475898
Nature. 1962 Dec 8;196:952-5
pubmed: 13966357
Phytochemistry. 2020 Apr;172:112289
pubmed: 32036187
Trends Plant Sci. 2016 Mar;21(3):187-198
pubmed: 26832945
Nat Plants. 2019 Oct;5(10):1043-1056
pubmed: 31527844
Nat Commun. 2016 Jul 12;7:12151
pubmed: 27402057
Proc Natl Acad Sci U S A. 2020 Feb 18;117(7):3874-3883
pubmed: 32015118
J Cell Biol. 1973 Feb;56(2):519-27
pubmed: 4566525
Proc Natl Acad Sci U S A. 2018 Feb 06;115(6):E1157-E1165
pubmed: 29358405
Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12923-8
pubmed: 10536024
PLoS One. 2022 Apr 12;17(4):e0261908
pubmed: 35413060
Fungal Divers. 2020 Sep;104(1):267-289
pubmed: 33364917
ISME J. 2019 Jul;13(7):1647-1658
pubmed: 30796337
Acta Histochem. 2000 Feb;102(1):21-35
pubmed: 10726162
Science. 2016 Jun 17;352(6292):1392-3
pubmed: 27313024
Nat Protoc. 2008;3(12):1820-31
pubmed: 18989260
Plant Cell Environ. 2021 Apr;44(4):1151-1164
pubmed: 33522606
Proc Natl Acad Sci U S A. 2020 Jan 14;117(2):1148-1159
pubmed: 31806755
Plant J. 2014 Aug;79(4):659-78
pubmed: 24450747
J Agric Food Chem. 2022 Jul 6;70(26):8010-8023
pubmed: 35729681
New Phytol. 2022 Nov;236(4):1393-1408
pubmed: 36028985
Plant Cell. 2017 Jun;29(6):1440-1459
pubmed: 28536145
Environ Microbiol. 2022 Dec;24(12):6238-6251
pubmed: 36229418
Phytopathology. 2019 Jan;109(1):6-16
pubmed: 30412012
Microbiome. 2021 May 10;9(1):105
pubmed: 33971961
PLoS Biol. 2022 Aug 11;20(8):e3001681
pubmed: 35951523
mBio. 2022 Apr 26;13(2):e0007922
pubmed: 35384699
FEMS Microbiol Ecol. 2020 Jun 1;96(6):
pubmed: 32275297
Biochemistry. 1964 Mar;3:426-31
pubmed: 14155109
Science. 1995 Jan 20;267(5196):371-4
pubmed: 7824933
Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5213-E5222
pubmed: 29686086
Bioresour Technol. 2003 Jan;86(1):65-72
pubmed: 12421011
Sci Adv. 2019 Sep 25;5(9):eaaw0759
pubmed: 31579818
Nat Rev Microbiol. 2004 Jan;2(1):43-56
pubmed: 15035008
Microbiol Spectr. 2022 Feb 23;10(1):e0232421
pubmed: 35019691
Appl Environ Microbiol. 2021 Apr 27;87(10):
pubmed: 33741622
Biochim Biophys Acta. 1995 Dec 13;1240(2):216-28
pubmed: 8541293
Am Nat. 2014 May;183(5):682-92
pubmed: 24739200
J Appl Microbiol. 2000 Jan;88(1):161-9
pubmed: 10735255
Proc Natl Acad Sci U S A. 2015 Feb 24;112(8):E911-20
pubmed: 25605935
FEMS Microbiol Ecol. 2009 Apr;68(1):1-13
pubmed: 19243436