CALU-3 lung cells three-dimensionally assembled onto CellFate® matrix present angiotensin-converting enzyme-2 activity.
3D model
ACE-2
COVID-19
lung epithelium
nanocellulose
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
08
08
2023
received:
15
05
2023
accepted:
30
08
2023
medline:
16
11
2023
pubmed:
11
9
2023
entrez:
11
9
2023
Statut:
ppublish
Résumé
Currently, there is a great need for the development of three-dimensional (3D) in vitro lung models. Particularly, the production of a suitable 3D model of pulmonary epithelium for understanding the pathophysiology of diseases such as the COVID-19 must consider the tissue architecture and presence, for example, of the angiotensin-converting enzyme-2 (ACE-2) in the cells. Different polymeric membranes are being used to support cell culturing, especially of lung cells, however, there is still no information about the culture of these cells onto bacterial nanocellulose (BNC) matrices. We have used the BNC matrix CellFate® as a support for the assembly of a 3D in vitro model of lung epithelium, composed of human lung fibroblasts (HLF) and lung adenocarcinoma cells (CALU-3). CellFate® matrices were made from bacterial fermentation resulting in a natural and biocompatible biopolymer. Cells were cultured onto CellFate® and maintained in a 5% CO
Substances chimiques
Angiotensins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3602-3611Subventions
Organisme : Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina
ID : FAPESC: No: 2020TR1063
Informations de copyright
© 2023 Wiley Periodicals LLC.
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