Methyl halide transferase-based gas reporters for quantification of filamentous bacteria in microdroplet emulsions.
Streptomyces
microfluidics
soil microbiology
Journal
Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801
Informations de publication
Date de publication:
28 09 2023
28 09 2023
Historique:
medline:
29
9
2023
pubmed:
12
9
2023
entrez:
12
9
2023
Statut:
ppublish
Résumé
The application of microfluidic techniques in experimental and environmental studies is a rapidly emerging field. Water-in-oil microdroplets can serve readily as controllable micro-vessels for studies that require spatial structure. In many applications, it is useful to monitor cell growth without breaking or disrupting the microdroplets. To this end, optical reporters based on color, fluorescence, or luminescence have been developed. However, optical reporters suffer from limitations when used in microdroplets such as inaccurate readings due to strong background interference or limited sensitivity during early growth stages. In addition, optical detection is typically not amenable to filamentous or biofilm-producing organisms that have significant nonlinear changes in opacity and light scattering during growth. To overcome such limitations, we show that volatile methyl halide gases produced by reporter cells expressing a methyl halide transferase (MHT) can serve as an alternative nonoptical detection approach suitable for microdroplets. In this study, an MHT-labeled
Identifiants
pubmed: 37699129
doi: 10.1128/aem.00764-23
pmc: PMC10537575
doi:
Substances chimiques
Emulsions
0
Gases
0
Transferases
EC 2.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0076423Subventions
Organisme : NIAID NIH HHS
ID : R01 AI080714
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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