Comprehensive autoantibody profiling in systemic autoimmunity by a highly-sensitive multiplex protein array.
autoantibody
dermatomyositis
polymyositis
proteomics
systemic sclerosis
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2023
2023
Historique:
received:
09
07
2023
accepted:
11
08
2023
medline:
14
9
2023
pubmed:
13
9
2023
entrez:
13
9
2023
Statut:
epublish
Résumé
Comprehensive autoantibody evaluation is essential for the management of autoimmune disorders. However, conventional methods suffer from poor sensitivity, low throughput, or limited availability. Here, using a proteome-wide human cDNA library, we developed a novel multiplex protein assay (autoantibody array assay; A-Cube) covering 65 antigens of 43 autoantibodies that are associated with systemic sclerosis (SSc) and polymyositis/dermatomyositis (PM/DM). The performance of A-Cube was validated against immunoprecipitation and established enzyme-linked immunosorbent assay. Further, through an evaluation of serum samples from 357 SSc and 172 PM/DM patients, A-Cube meticulously illustrated a diverse autoantibody landscape in these diseases. The wide coverage and high sensitivity of A-Cube also allowed the overlap and correlation analysis between multiple autoantibodies. Lastly, reviewing the cases with distinct autoantibody profiles by A-Cube underscored the importance of thorough autoantibody detection. Together, these data highlighted the utility of A-Cube as well as the clinical relevance of autoantibody profiles in SSc and PM/DM.
Identifiants
pubmed: 37701440
doi: 10.3389/fimmu.2023.1255540
pmc: PMC10493387
doi:
Substances chimiques
Autoantibodies
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1255540Informations de copyright
Copyright © 2023 Kuzumi, Norimatsu, Matsuda, Ono, Okumura, Kogo, Goshima, Fukasawa, Fushida, Horii, Yamashita, Yoshizaki-Ogawa, Yamaguchi, Matsushita, Sato and Yoshizaki.
Déclaration de conflit d'intérêts
Authors CO, TO, EK, KY, and NG are employed by the company ProteoBridge Corporation. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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