Human OPRM1 and murine Oprm1 promoter driven viral constructs for genetic access to μ-opioidergic cell types.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 09 2023
13 09 2023
Historique:
received:
26
09
2022
accepted:
31
08
2023
medline:
15
9
2023
pubmed:
14
9
2023
entrez:
13
9
2023
Statut:
epublish
Résumé
With concurrent global epidemics of chronic pain and opioid use disorders, there is a critical need to identify, target and manipulate specific cell populations expressing the mu-opioid receptor (MOR). However, available tools and transgenic models for gaining long-term genetic access to MOR+ neural cell types and circuits involved in modulating pain, analgesia and addiction across species are limited. To address this, we developed a catalog of MOR promoter (MORp) based constructs packaged into adeno-associated viral vectors that drive transgene expression in MOR+ cells. MORp constructs designed from promoter regions upstream of the mouse Oprm1 gene (mMORp) were validated for transduction efficiency and selectivity in endogenous MOR+ neurons in the brain, spinal cord, and periphery of mice, with additional studies revealing robust expression in rats, shrews, and human induced pluripotent stem cell (iPSC)-derived nociceptors. The use of mMORp for in vivo fiber photometry, behavioral chemogenetics, and intersectional genetic strategies is also demonstrated. Lastly, a human designed MORp (hMORp) efficiently transduced macaque cortical OPRM1+ cells. Together, our MORp toolkit provides researchers cell type specific genetic access to target and functionally manipulate mu-opioidergic neurons across a range of vertebrate species and translational models for pain, addiction, and neuropsychiatric disorders.
Identifiants
pubmed: 37704594
doi: 10.1038/s41467-023-41407-2
pii: 10.1038/s41467-023-41407-2
pmc: PMC10499891
doi:
Substances chimiques
OPRM1 protein, human
0
Receptors, Opioid
0
Receptors, Opioid, mu
0
Oprm protein, mouse
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5632Subventions
Organisme : NIDA NIH HHS
ID : F32 DA055458
Pays : United States
Organisme : NIDA NIH HHS
ID : R00 DA043609
Pays : United States
Organisme : NIGMS NIH HHS
ID : DP2 GM140923
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA056599
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA054374
Pays : United States
Informations de copyright
© 2023. Springer Nature Limited.
Références
Neuron. 2021 May 5;109(9):1426-1429
pubmed: 33957072
Sci Transl Med. 2021 Nov 10;13(619):eabj9837
pubmed: 34757806
Neuroscience. 2005;133(1):97-103
pubmed: 15893634
Synapse. 2013 May;67(5):216-23
pubmed: 23280810
Pain. 1987 Jul;30(1):103-114
pubmed: 3614974
Neuroscience. 2000;98(2):221-31
pubmed: 10854753
J Neurosci. 2020 Nov 18;40(47):9043-9054
pubmed: 33067362
Brain. 2008 May;131(Pt 5):1171-96
pubmed: 18048446
J Pharmacol Exp Ther. 2003 Jul;306(1):179-86
pubmed: 12676881
Cell Mol Neurobiol. 2021 Jul;41(5):1119-1129
pubmed: 33400082
Annu Rev Pharmacol Toxicol. 2011;51:75-97
pubmed: 20868272
PLoS One. 2016 Jun 16;11(6):e0156805
pubmed: 27309348
Eur J Pharmacol. 1999 Jun 11;374(1):77-84
pubmed: 10422643
Anesthesiology. 2018 Jun;128(6):1220-1236
pubmed: 29601322
Pain. 2020 Jul;161(7):1636-1649
pubmed: 32102022
Brain Res. 1996 Mar 25;713(1-2):99-107
pubmed: 8724980
Neuron. 2021 May 5;109(9):1449-1464.e13
pubmed: 33789083
Annu Rev Neurosci. 2018 Jul 8;41:453-473
pubmed: 29852083
Nat Rev Drug Discov. 2018 Sep;17(9):641-659
pubmed: 30093643
Curr Protoc Hum Genet. 2018 Jan 24;96:21.12.1-21.12.12
pubmed: 29364522
J Neurosci. 2023 Mar 8;43(10):1692-1713
pubmed: 36717230
Biochem Biophys Res Commun. 1997 May 19;234(2):351-7
pubmed: 9177274
J Neurosci. 2005 Feb 9;25(6):1366-74
pubmed: 15703390
eNeuro. 2020 May 29;7(3):
pubmed: 32381649
Nat Neurosci. 2013 Feb;16(2):183-92
pubmed: 23292683
Trends Neurosci. 1988 Jul;11(7):308-14
pubmed: 2465635
Front Psychiatry. 2019 Jan 04;9:726
pubmed: 30662412
N Engl J Med. 2017 Nov 2;377(18):1713-1722
pubmed: 29091557
Nucleic Acids Res. 2003 Jul 1;31(13):3651-3
pubmed: 12824386
Nucleic Acids Res. 2006;34(22):6392-403
pubmed: 17130167
Neuroscience. 2014 Sep 26;277:595-609
pubmed: 25086313
PLoS One. 2013;8(4):e60365
pubmed: 23565235
J Neurosci Methods. 1994 Jul;53(1):55-63
pubmed: 7990513
Front Mol Neurosci. 2022 Jul 11;15:932189
pubmed: 35898697
Front Neuroanat. 2019 Nov 26;13:93
pubmed: 31849618
Eur J Pharmacol. 1986 Mar 4;121(3):355-65
pubmed: 2870933
Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):13117-22
pubmed: 20615975
Brain. 2021 Jun 22;144(5):1312-1335
pubmed: 34128530
STAR Protoc. 2021 Feb 08;2(1):100333
pubmed: 33615276
Mol Pharmacol. 2013 Nov;84(5):687-95
pubmed: 23960100
J Neurosci Res. 2022 Jan;100(1):48-65
pubmed: 33957003
Nature. 2021 Oct;598(7882):646-651
pubmed: 34646022
Exp Neurol. 1998 Apr;150(2):183-94
pubmed: 9527887
Eur J Anaesthesiol. 2004 Mar;21(3):179-85
pubmed: 15055889
Nat Neurosci. 2017 Aug;20(8):1172-1179
pubmed: 28671695
ACS Chem Neurosci. 2018 Apr 18;9(4):684-690
pubmed: 29266926
Nat Neurosci. 2014 Jan;17(1):131-43
pubmed: 24316888
Nat Methods. 2022 Aug;19(8):976-985
pubmed: 35879607
J Neurosci Methods. 1985 Jun;14(1):69-76
pubmed: 4033190
J Neuromuscul Dis. 2019;6(3):307-317
pubmed: 31381526
Nat Rev Neurosci. 2018 Aug;19(8):499-514
pubmed: 29934561
Neuron. 2011 Sep 22;71(6):995-1013
pubmed: 21943598
J Comp Neurol. 1994 Dec 15;350(3):412-38
pubmed: 7884049
Physiol Behav. 2012 Jun 25;106(4):562-8
pubmed: 22504494
EBioMedicine. 2019 Jan;39:401-408
pubmed: 30503201
Nat Med. 2017 Feb;23(2):164-173
pubmed: 28092666
Neuron. 2020 Dec 23;108(6):1075-1090.e6
pubmed: 33080229
J Neurosci. 2007 Feb 14;27(7):1543-51
pubmed: 17301163
Pharmacol Biochem Behav. 2021 Feb;201:173093
pubmed: 33385438
Bio Protoc. 2021 Aug 05;11(15):e4102
pubmed: 34458396
Nat Neurosci. 2016 Dec;19(12):1743-1749
pubmed: 27798629
Mol Ther. 2006 Mar;13(3):528-37
pubmed: 16413228
J Comp Neurol. 2001 Apr 30;433(2):272-96
pubmed: 11283964
Science. 2019 Jan 18;363(6424):276-281
pubmed: 30655440
J Neurosci. 2016 Jun 15;36(24):6488-96
pubmed: 27307236
Neuron. 2018 Apr 4;98(1):90-108.e5
pubmed: 29576387
Am Fam Physician. 2006 Oct 15;74(8):1347-54
pubmed: 17087429
Neuropharmacology. 2001 Oct;41(5):574-81
pubmed: 11587712
J Neurosci. 2008 Jan 23;28(4):904-13
pubmed: 18216198
Neural Regen Res. 2021 Apr;16(4):653-658
pubmed: 33063716
Am J Physiol. 1997 Apr;272(4 Pt 2):R1028-32
pubmed: 9139997
J Neurovirol. 2017 Feb;23(1):47-66
pubmed: 27873219
Respir Physiol Neurobiol. 2017 Jan;235:71-78
pubmed: 27743812
Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):
pubmed: 34074761
J Biol Chem. 2004 May 7;279(19):19764-74
pubmed: 14998994
Pharmacol Rev. 2013 Sep 27;65(4):1257-317
pubmed: 24076545
Neuropharmacology. 2018 Mar 15;131:282-290
pubmed: 29247677
Nucleic Acids Res. 2015 Jan;43(Database issue):D92-6
pubmed: 25378343
Br J Anaesth. 2010 Jun;104(6):698-704
pubmed: 20413378
J Pharmacol Exp Ther. 1998 Jan;284(1):378-87
pubmed: 9435201
N Engl J Med. 2013 Apr 11;368(15):1388-97
pubmed: 23574118
Genesis. 2020 Jan;58(1):e23341
pubmed: 31651080
Nat Methods. 2023 May;20(5):682-685
pubmed: 36973548
J Comp Neurol. 2001 May 14;433(4):471-85
pubmed: 11304712
Eur J Pharmacol. 1980 Oct 17;67(2-3):313-6
pubmed: 6893963
Nat Neurosci. 2013 Mar;16(3):332-9
pubmed: 23354330
Sci Rep. 2015 Sep 21;5:14306
pubmed: 26387932
J Biol Chem. 2021 Jan-Jun;296:100277
pubmed: 33428940
J Alzheimers Dis. 2020;74(2):563-578
pubmed: 32065798
Cell. 2022 Jul 21;185(15):2739-2755
pubmed: 35868277
J Neurosci Res. 2022 Jan;100(1):66-98
pubmed: 33314372
eNeuro. 2022 Jun 21;:
pubmed: 35728954
Nature. 2018 Nov;563(7729):72-78
pubmed: 30382198
Elife. 2019 Nov 11;8:
pubmed: 31710287
Biol Psychiatry. 2020 Jan 1;87(1):74-81
pubmed: 31399256
Stem Cell Reports. 2023 Apr 11;18(4):1030-1047
pubmed: 37044067
Front Pharmacol. 2018 Dec 19;9:1478
pubmed: 30618766
Elife. 2018 Sep 07;7:
pubmed: 30192227
Nat Commun. 2019 Sep 13;10(1):4169
pubmed: 31519873
Nature. 2010 Nov 11;468(7321):270-6
pubmed: 21068836
Nat Rev Neurosci. 2023 Apr;24(4):252-265
pubmed: 36658346
Annu Rev Neurosci. 2022 Jul 8;45:447-469
pubmed: 35440143
Proc Natl Acad Sci U S A. 2007 Aug 14;104(33):13525-30
pubmed: 17686976
Cell Metab. 2020 Feb 4;31(2):351-362.e5
pubmed: 31928886
Methods Mol Biol. 2008;437:51-91
pubmed: 18369962
Cell Rep Methods. 2021 May 24;1(1):
pubmed: 34318289
Mol Ther Methods Clin Dev. 2019 Nov 26;17:69-82
pubmed: 31890742
Annu Rev Neurosci. 2018 Jul 8;41:323-348
pubmed: 29709207
PLoS One. 2022 Dec 19;17(12):e0270317
pubmed: 36534642
Neuropharmacology. 2014 Jan;76 Pt B:204-17
pubmed: 24035914
Elife. 2023 Jan 20;12:
pubmed: 36661218