Recombinant expression and subcellular targeting of the particulate methane monooxygenase (pMMO) protein components in plants.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 09 2023
15 09 2023
Historique:
received:
28
03
2023
accepted:
07
09
2023
medline:
18
9
2023
pubmed:
16
9
2023
entrez:
15
9
2023
Statut:
epublish
Résumé
Methane is a potent greenhouse gas, which has contributed to approximately a fifth of global warming since pre-industrial times. The agricultural sector produces significant methane emissions, especially from livestock, waste management and rice cultivation. Rice fields alone generate around 9% of total anthropogenic emissions. Methane is produced in waterlogged paddy fields by methanogenic archaea, and transported to the atmosphere through the aerenchyma tissue of rice plants. Thus, bioengineering rice with catalysts to detoxify methane en route could contribute to an efficient emission mitigation strategy. Particulate methane monooxygenase (pMMO) is the predominant methane catalyst found in nature, and is an enzyme complex expressed by methanotrophic bacteria. Recombinant expression of pMMO has been challenging, potentially due to its membrane localization, multimeric structure, and polycistronic operon. Here we show the first steps towards the engineering of plants for methane detoxification with the three pMMO subunits expressed in the model systems tobacco and Arabidopsis. Membrane topology and protein-protein interactions were consistent with correct folding and assembly of the pMMO subunits on the plant ER. Moreover, a synthetic self-cleaving polypeptide resulted in simultaneous expression of all three subunits, although low expression levels precluded more detailed structural investigation. The work presents plant cells as a novel heterologous system for pMMO allowing for protein expression and modification.
Identifiants
pubmed: 37714899
doi: 10.1038/s41598-023-42224-9
pii: 10.1038/s41598-023-42224-9
pmc: PMC10504283
doi:
Substances chimiques
methane monooxygenase
EC 1.14.13.25
Dust
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15337Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/W011166/1
Pays : United Kingdom
Informations de copyright
© 2023. Springer Nature Limited.
Références
Plant Physiol. 2015 Jul;168(3):776-87
pubmed: 25971551
Science. 2022 Mar 18;375(6586):1287-1291
pubmed: 35298269
Nucleic Acids Res. 2015 Jul 1;43(W1):W408-12
pubmed: 25943549
J Bacteriol. 1996 Feb;178(4):1018-29
pubmed: 8576034
Plant J. 2003 Aug;35(4):545-55
pubmed: 12904216
J Biol Chem. 2018 Jul 6;293(27):10457-10465
pubmed: 29739854
Plant Physiol. 1994 Oct;106(2):739-745
pubmed: 12232366
Plant Cell Physiol. 1999 Dec;40(12):1253-61
pubmed: 10682347
Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):10902-7
pubmed: 27621477
Plant J. 1993 Nov;4(5):793-800
pubmed: 8275099
Appl Environ Microbiol. 2018 Mar 1;84(6):
pubmed: 29305514
Arch Microbiol. 2000 May-Jun;173(5-6):325-32
pubmed: 10896210
Front Plant Sci. 2018 Sep 19;9:1379
pubmed: 30283486
J Inorg Biochem. 2019 Jul;196:110691
pubmed: 31063931
Curr Opin Cell Biol. 2006 Aug;18(4):358-64
pubmed: 16806883
Plant J. 1998 Dec;16(6):735-43
pubmed: 10069079
Sci Rep. 2017 May 19;7(1):2193
pubmed: 28526819
Biochemistry. 2005 Aug 23;44(33):10954-65
pubmed: 16101279
Plant Cell. 2010 Apr;22(4):1333-43
pubmed: 20424177
EMBO J. 1992 Jun;11(6):2345-55
pubmed: 1376250
Curr Biol. 2014 Jun 16;24(12):1397-1405
pubmed: 24909329
Nature. 2007 Nov 29;450(7170):663-9
pubmed: 18046402
Biochemistry. 2015 Apr 14;54(14):2283-94
pubmed: 25806595
J Biol Chem. 1994 May 27;269(21):14995-5005
pubmed: 8195135
Plant Cell. 2010 Aug;22(8):2796-811
pubmed: 20699392
J Microsc. 2008 Aug;231(2):299-316
pubmed: 18778428
AoB Plants. 2012;2012:pls031
pubmed: 23119138
Plant Physiol. 1990 Sep;94(1):59-66
pubmed: 16667719
Plant J. 2003 Mar;33(5):949-56
pubmed: 12609035
Funct Plant Biol. 2009 May;36(5):409-430
pubmed: 32688656
Arch Biochem Biophys. 1995 Aug 20;321(2):421-8
pubmed: 7646068
Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3820-5
pubmed: 12634423
Plant Physiol. 2015 Nov;169(3):1933-45
pubmed: 26353761
Biochemistry. 2008 Jul 1;47(26):6793-801
pubmed: 18540635
Philos Trans A Math Phys Eng Sci. 2011 May 28;369(1943):2058-72
pubmed: 21502176
Nature. 2005 Mar 10;434(7030):177-82
pubmed: 15674245
Trends Plant Sci. 2005 Mar;10(3):103-5
pubmed: 15749466
Microbiol Rev. 1996 Jun;60(2):439-71
pubmed: 8801441
J Exp Bot. 2020 Mar 25;71(6):1757-1762
pubmed: 31811712
Trends Biochem Sci. 2018 Jan;43(1):32-43
pubmed: 29153511
Plant Cell. 2000 Nov;12(11):2201-18
pubmed: 11090219
J Gen Virol. 2001 May;82(Pt 5):1013-1025
pubmed: 11297676
Mol Interv. 2005 Aug;5(4):216-25
pubmed: 16123536
Antioxid Redox Signal. 2016 May 1;24(13):752-62
pubmed: 26154420
J Exp Bot. 2016 Jul;67(14):4195-207
pubmed: 27208541
Plant Physiol. 2000 May;123(1):287-96
pubmed: 10806245
Traffic. 2006 Dec;7(12):1701-23
pubmed: 17118121
Nat Commun. 2019 Feb 28;10(1):984
pubmed: 30816109
J Biol Chem. 2004 Mar 26;279(13):13044-53
pubmed: 14722062
Biochemistry. 2011 Nov 29;50(47):10231-40
pubmed: 22013879
Methods Enzymol. 2011;495:135-47
pubmed: 21419919
Plant J. 2009 Feb;57(3):534-41
pubmed: 18939964
Biometals. 1998 Sep;11(3):229-34
pubmed: 9850566
FEMS Microbiol Lett. 2006 Oct;263(2):136-41
pubmed: 16978347
Cell Rep. 2016 Feb 2;14(4):679-685
pubmed: 26804901
Nat Protoc. 2006;1(4):2019-25
pubmed: 17487191
J Microsc. 2015 Apr;258(1):68-78
pubmed: 25664385
Plant Biotechnol J. 2005 Mar;3(2):141-55
pubmed: 17173615
Cell Mol Life Sci. 2016 Jan;73(1):79-94
pubmed: 26433683
Bioinformatics. 2017 Nov 01;33(21):3387-3395
pubmed: 29036616
Chem Soc Rev. 2021 Mar 7;50(5):3424-3436
pubmed: 33491685
Microorganisms. 2021 Apr 12;9(4):
pubmed: 33921272
J Am Chem Soc. 2016 Aug 3;138(30):9327-40
pubmed: 27366961
Sci Adv. 2021 Mar 31;7(14):
pubmed: 33789891