Recombinase-independent AAV for anterograde transsynaptic tracing.
AAV
Neural-circuit
Neuroanatomy
Transsynaptic
Viral-tracing
Journal
Molecular brain
ISSN: 1756-6606
Titre abrégé: Mol Brain
Pays: England
ID NLM: 101468876
Informations de publication
Date de publication:
15 09 2023
15 09 2023
Historique:
received:
05
07
2023
accepted:
03
09
2023
medline:
18
9
2023
pubmed:
16
9
2023
entrez:
15
9
2023
Statut:
epublish
Résumé
Viral transsynaptic labeling has become indispensable for investigating the functional connectivity of neural circuits in the mammalian brain. Adeno-associated virus serotype 1 (AAV1) allows for anterograde transneuronal labeling and manipulation of postsynaptic neurons. However, it is limited to delivering an AAV1 expressing a recombinase which relies on using transgenic animals or genetic access to postsynaptic neurons. We reasoned that a strong expression level could overcome this limitation. To this end, we used a self-complementary AAV of serotype 1 (scAAV1) under a strong promoter (CAG). We demonstrated the anterograde transneuronal efficiency of scAAV1 by delivering a fluorescent marker in mouse retina-superior colliculus and thalamic-amygdala pathways in a recombinase-independent manner in the mouse brain. In addition to investigating neuronal connectivity, anterograde transsynaptic AAVs with a strong promoter may be suitable for functional mapping and imaging.
Identifiants
pubmed: 37715263
doi: 10.1186/s13041-023-01053-7
pii: 10.1186/s13041-023-01053-7
pmc: PMC10504749
doi:
Substances chimiques
Recombinases
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
66Subventions
Organisme : European Research Council
ID : 22736
Pays : International
Informations de copyright
© 2023. Min Zhuo, Bong-Kiun Kaang and BioMed central Ltd.
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