Cep104 is a component of the centriole distal tip complex that regulates centriole growth and contributes to Drosophila spermiogenesis.
axoneme
centriole
centrosome
distal tip complex
microtubule
spermiogenesis
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
09 10 2023
09 10 2023
Historique:
received:
14
03
2023
revised:
21
07
2023
accepted:
24
08
2023
pmc-release:
09
10
2024
medline:
26
10
2023
pubmed:
21
9
2023
entrez:
20
9
2023
Statut:
ppublish
Résumé
Proper centrosome number and function relies on the accurate assembly of centrioles, barrel-shaped structures that form the core duplicating elements of the organelle. The growth of centrioles is regulated in a cell cycle-dependent manner; while new daughter centrioles elongate during the S/G2/M phase, mature mother centrioles maintain their length throughout the cell cycle. Centriole length is controlled by the synchronized growth of the microtubules that ensheathe the centriole barrel. Although proteins exist that target the growing distal tips of centrioles, such as CP110 and Cep97, these proteins are generally thought to suppress centriolar microtubule growth, suggesting that distal tips may also contain unidentified counteracting factors that facilitate microtubule polymerization. Currently, a mechanistic understanding of how distal tip proteins balance microtubule growth and shrinkage to either promote daughter centriole elongation or maintain centriole length is lacking. Using a proximity-labeling screen in Drosophila cells, we identified Cep104 as a novel component of a group of evolutionarily conserved proteins that we collectively refer to as the distal tip complex (DTC). We found that Cep104 regulates centriole growth and promotes centriole elongation through its microtubule-binding TOG domain. Furthermore, analysis of Cep104 null flies revealed that Cep104 and Cep97 cooperate during spermiogenesis to align spermatids and coordinate individualization. Lastly, we mapped the complete DTC interactome and showed that Cep97 is the central scaffolding unit required to recruit DTC components to the distal tip of centrioles.
Identifiants
pubmed: 37729913
pii: S0960-9822(23)01156-9
doi: 10.1016/j.cub.2023.08.075
pmc: PMC10591971
mid: NIHMS1929446
pii:
doi:
Substances chimiques
Microtubule-Associated Proteins
0
Cell Cycle Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
4202-4216.e9Subventions
Organisme : NCI NIH HHS
ID : P30 CA023074
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136265
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA HL006126
Pays : United States
Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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