Salmonid polysialyltransferases to generate a variety of sialic acid polymers.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
20 09 2023
20 09 2023
Historique:
received:
29
06
2023
accepted:
05
09
2023
medline:
22
9
2023
pubmed:
21
9
2023
entrez:
20
9
2023
Statut:
epublish
Résumé
The human polysialyltransferases ST8Sia II and ST8Sia IV catalyze the transfer of several Neu5Ac residues onto glycoproteins forming homopolymers with essential roles during different physiological processes. In salmonids, heterogeneous set of sialic acids polymers have been described in ovary and on eggs cell surface and three genes st8sia4, st8sia2-r1 and st8sia2-r2 were identified that could be implicated in these heteropolymers. The three polysialyltransferases from the salmonid Coregonus maraena were cloned, recombinantly expressed in HEK293 cells and the ST8Sia IV was biochemically characterized. The MicroPlate Sialyltransferase Assay and the non-natural donor substrate CMP-SiaNAl were used to demonstrate enzyme activity and optimize polysialylation reactions. Polysialylation was also carried out with natural donor substrates CMP-Neu5Ac, CMP-Neu5Gc and CMP-Kdn in cell-free and cell-based assays and structural analyses of polysialylated products using the anti-polySia monoclonal antibody 735 and endoneuraminidase N and HPLC approaches. Our data highlighted distinct specificities of human and salmonid polysialyltransferases with notable differences in donor substrates use and the capacity of fish enzymes to generate heteropolymers. This study further suggested an evolution of the biological functions of polySia. C. maraena ST8Sia IV of particular interest to modify glycoproteins with a variety of polySia chains.
Identifiants
pubmed: 37730806
doi: 10.1038/s41598-023-42095-0
pii: 10.1038/s41598-023-42095-0
pmc: PMC10511417
doi:
Substances chimiques
N-Acetylneuraminic Acid
GZP2782OP0
polysialic acid
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15610Informations de copyright
© 2023. Springer Nature Limited.
Références
BMC Evol Biol. 2008 Sep 23;8:258
pubmed: 18811928
Front Vet Sci. 2022 May 03;9:889635
pubmed: 35591870
J Biol Chem. 1986 Sep 5;261(25):11550-7
pubmed: 3745155
J Biol Chem. 2012 Feb 24;287(9):6441-53
pubmed: 22184126
Int J Mol Sci. 2020 Jan 14;21(2):
pubmed: 31947579
J Biol Chem. 1988 Dec 5;263(34):18269-76
pubmed: 3142874
Methods Enzymol. 2003;362:543-60
pubmed: 12968387
Mol Aspects Med. 2021 Jun;79:100892
pubmed: 32863045
J Biol Chem. 1993 Nov 5;268(31):23675-84
pubmed: 8226894
Biology (Basel). 2017 Apr 27;6(2):
pubmed: 28448440
Indian J Biochem Biophys. 1997 Feb-Apr;34(1-2):157-65
pubmed: 9343944
Glycoconj J. 1994 Oct;11(5):493-9
pubmed: 7696852
J Biol Chem. 2009 Jun 5;284(23):15505-16
pubmed: 19336400
J Mol Graph Model. 2006 Oct;25(2):247-60
pubmed: 16458552
J Biol Chem. 1994 Mar 25;269(12):8762-71
pubmed: 8132608
Analyst. 2020 Jul 7;145(13):4512-4521
pubmed: 32412559
J Biol Chem. 1986 Apr 25;261(12):5262-9
pubmed: 3514613
Glycobiology. 2016 Nov;26(11):1151-1156
pubmed: 27543325
Bioconjug Chem. 2018 Oct 17;29(10):3377-3384
pubmed: 30192128
Chembiochem. 2017 Jul 4;18(13):1251-1259
pubmed: 28395125
Cells. 2020 Oct 31;9(11):
pubmed: 33142835
Int J Mol Sci. 2019 Jan 31;20(3):
pubmed: 30709055
Front Cell Dev Biol. 2022 Oct 20;10:982931
pubmed: 36340022
Front Immunol. 2022 Feb 11;12:823637
pubmed: 35222358
Glycoconj J. 2006 Jul;23(5-6):423-36
pubmed: 16897183
Cell Mol Life Sci. 2012 Apr;69(7):1179-91
pubmed: 22068610
Annu Rev Biochem. 2006;75:403-33
pubmed: 16756497
Glycobiology. 2005 Aug;15(8):805-17
pubmed: 15843597
Physiol Rev. 2014 Apr;94(2):461-518
pubmed: 24692354
Biology (Basel). 2022 Dec 20;12(1):
pubmed: 36671698
Front Neurosci. 2020 Dec 04;14:591738
pubmed: 33343287
J Biol Chem. 2013 Jun 28;288(26):18825-33
pubmed: 23671285
Int J Mol Sci. 2020 Feb 26;21(5):
pubmed: 32111064
Carbohydr Polym. 2019 Mar 15;208:32-41
pubmed: 30658806
Biochem Biophys Res Commun. 2022 Aug 20;617(Pt 1):16-21
pubmed: 35667241
Cells. 2021 May 29;10(6):
pubmed: 34072405
Chem Rev. 2002 Feb;102(2):439-69
pubmed: 11841250
Anal Biochem. 2021 Dec 15;635:114426
pubmed: 34687617
Biochimie. 2001 Aug;83(8):727-37
pubmed: 11530204
Int J Mol Sci. 2018 Jun 05;19(6):
pubmed: 29874880
Glycoconj J. 2023 Aug;40(4):473-492
pubmed: 37247156
PLoS One. 2015 Mar 30;10(3):e0123960
pubmed: 25822229
Nat Struct Mol Biol. 2005 Jan;12(1):90-6
pubmed: 15608653
J Biol Chem. 2013 Nov 22;288(47):33784-33796
pubmed: 24100042
Nat Chem Biol. 2018 Feb;14(2):156-162
pubmed: 29251719
J Biochem. 2006 Nov;140(5):687-701
pubmed: 17023684