Characterization of vestibular schwannoma tissues using liquid chromatography-tandem mass spectrometry analysis of specific peptide fragments separated by in-sample tryptic protein digestion followed by mathematical analysis.
In-sample protein digestion
liquid chromatography
principal component analysis
tandem mass spectrometry
vestibular schwannomas
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
revised:
12
09
2023
received:
28
07
2023
accepted:
15
09
2023
medline:
27
11
2023
pubmed:
22
9
2023
entrez:
22
9
2023
Statut:
ppublish
Résumé
Vestibular schwannoma is the most common benign neoplasm of the cerebellopontine angle. Its first symptoms include hearing loss, tinnitus, and vestibular symptoms, followed by cerebellar and brainstem symptoms, along with palsy of the adjacent cranial nerves. However, the clinical picture has unpredictable dynamics and currently, there are no reliable predictors of tumor behavior. Hence, it is desirable to have a fast routine method for analysis of vestibular schwannoma tissues at the molecular level. The major objective of this study was to verify whether a technique using in-sample specific protein digestion with trypsin would have the potential to provide a proteomic characterization of these pathological tissues. The achieved results showed that the use of this approach with subsequent liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of released peptides allowed a fast identification of a considerable number of proteins in two differential parts of vestibular schwannoma tissue as well as in tissues of control healthy samples. Furthermore, mathematical analysis of MS data was able to discriminate between pathological vestibular schwannoma tissues and healthy tissues. Thus, in-sample protein digestion combined with LC-MS/MS separation and identification of released specific peptides followed by mathematical analysis appears to have the potential for routine characterization of vestibular schwannomas at the molecular level. Data are available via ProteomeXchange with identifier PXD045261.
Identifiants
pubmed: 37735989
doi: 10.1002/jssc.202300543
doi:
Substances chimiques
Peptide Fragments
0
Peptides
0
Trypsin
EC 3.4.21.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300543Subventions
Organisme : Grant Agency of Charles University in Prague (GAUK)
ID : 194423
Organisme : Grant Agency of Charles University in Prague (GAUK)
ID : 874120
Organisme : Czech Science Foundation (GACR)
ID : 19-08241S
Organisme : Ministry of Health of the Czech Republic
ID : NV19-06-00189
Organisme : Ministry of Health of the Czech Republic
ID : NV20-08-00311
Organisme : Charles University in Prague
ID : Cooperatio ONCO
Organisme : Czech Academy of Sciences
ID : RVO 61388963
Organisme : Ministry of Education, Youth and Sports of the Czech Republic
ID : LUC23138
Informations de copyright
© 2023 The Authors. Journal of Separation Science published by Wiley-VCH GmbH.
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