Humic acid-dependent respiratory growth of Methanosarcina acetivorans involves pyrroloquinoline quinone.
Journal
The ISME journal
ISSN: 1751-7370
Titre abrégé: ISME J
Pays: England
ID NLM: 101301086
Informations de publication
Date de publication:
11 2023
11 2023
Historique:
received:
08
05
2022
accepted:
14
09
2023
revised:
12
09
2023
pmc-release:
01
11
2024
medline:
23
10
2023
pubmed:
22
9
2023
entrez:
22
9
2023
Statut:
ppublish
Résumé
Although microbial humus respiration plays a critical role in organic matter decomposition and biogeochemical cycling of elements in diverse anoxic environments, the role of methane-producing species (methanogens) is not well defined. Here we report that a major fraction of humus, humic acid reduction enhanced the growth of Methanosarcina acetivorans above that attributed to methanogenesis when utilizing the energy sources methanol or acetate, results which showed both respiratory and fermentative modes of energy conservation. Growth characteristics with methanol were the same for an identically cultured mutant deleted for the gene encoding a multi-heme cytochrome c (MmcA), results indicating MmcA is not essential for respiratory electron transport to humic acid. Transcriptomic analyses revealed that growth with humic acid promoted the upregulation of genes annotated as cell surface pyrroloquinoline quinone (PQQ)-binding proteins. Furthermore, PQQ isolated from the membrane fraction was more abundant in humic acid-respiring cells, and the addition of PQQ improved efficiency of the extracellular electron transport. Given that the PQQ-binding proteins are widely distributed in methanogens, the findings extend current understanding of microbial humus respiration in the context of global methane dynamics.
Identifiants
pubmed: 37737251
doi: 10.1038/s41396-023-01520-y
pii: 10.1038/s41396-023-01520-y
pmc: PMC10579383
doi:
Substances chimiques
Methanol
Y4S76JWI15
Humic Substances
0
PQQ Cofactor
72909-34-3
Methane
OP0UW79H66
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2103-2111Informations de copyright
© 2023. The Author(s), under exclusive licence to International Society for Microbial Ecology.
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