Pichia pastoris growth-coupled heme biosynthesis analysis using metabolic modelling.

Glycerol Leghemoglobin Methanol Heme

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
22 09 2023
Historique:
received: 30 03 2023
accepted: 15 09 2023
medline: 25 9 2023
pubmed: 23 9 2023
entrez: 22 9 2023
Statut: epublish

Résumé

Soy leghemoglobin is one of the most important and key ingredients in plant-based meat substitutes that can imitate the colour and flavour of the meat. To improve the high-yield production of leghemoglobin protein and its main component-heme in the yeast Pichia pastoris, glycerol and methanol cultivation conditions were studied. Additionally, in-silico metabolic modelling analysis of growth-coupled enzyme quantity, suggests metabolic gene up/down-regulation strategies for heme production. First, cultivations and metabolic modelling analysis of P. pastoris were performed on glycerol and methanol in different growth media. Glycerol cultivation uptake and production rates can be increased by 50% according to metabolic modelling results, but methanol cultivation-is near the theoretical maximum. Growth-coupled metabolic optimisation results revealed the best feasible upregulation (33 reactions) (1.47% of total reactions) and 66 downregulation/deletion (2.98% of total) reaction suggestions. Finally, we describe reaction regulation suggestions with the highest potential to increase heme production yields.

Identifiants

DOI: 10.1038/s41598-023-42865-w PMID: 37739976 PMC: PMC10516909
pubmed: 37739976
doi: 10.1038/s41598-023-42865-w
pii: 10.1038/s41598-023-42865-w
pmc: PMC10516909
doi:

Substances chimiques

Glycerol PDC6A3C0OX
Leghemoglobin 0
Methanol Y4S76JWI15
Heme 42VZT0U6YR

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

15816

Informations de copyright

© 2023. Springer Nature Limited.

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Auteurs

Agris Pentjuss (A)

Microbiology and Biotechnology Institute, University of Latvia, Jelgavas Street 1, Riga, 1004, Latvia. agris.pentjuss@gmail.com.
Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, Riga, 1006, Latvia. agris.pentjuss@gmail.com.

Emils Bolmanis (E)

Latvian Biomedical Research and Study Centre, Ratsupites Street 1 K-1, Riga, 1067, Latvia.

Anastasija Suleiko (A)

Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, Riga, 1006, Latvia.
Bioreactors.Net AS, Dzerbenes Street 27, Riga, 1006, Latvia.

Elina Didrihsone (E)

Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, Riga, 1006, Latvia.

Arturs Suleiko (A)

Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, Riga, 1006, Latvia.
Bioreactors.Net AS, Dzerbenes Street 27, Riga, 1006, Latvia.

Konstantins Dubencovs (K)

Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, Riga, 1006, Latvia.
Bioreactors.Net AS, Dzerbenes Street 27, Riga, 1006, Latvia.

Janis Liepins (J)

Microbiology and Biotechnology Institute, University of Latvia, Jelgavas Street 1, Riga, 1004, Latvia.

Andris Kazaks (A)

Latvian Biomedical Research and Study Centre, Ratsupites Street 1 K-1, Riga, 1067, Latvia.

Juris Vanags (J)

Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, Riga, 1006, Latvia.
Bioreactors.Net AS, Dzerbenes Street 27, Riga, 1006, Latvia.

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Classifications MeSH

questionsmedicales.fr › Glucides › Polyols › Dérivés du glycérol › Glycérol › Pichia pastoris growth-coupled heme...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Leghémoglobine › Pichia pastoris growth-coupled heme...
questionsmedicales.fr › Composés chimiques organiques › Alcools › Méthanol › Pichia pastoris growth-coupled heme...
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Porphyrines › Métalloporphyrines › Hème › Pichia pastoris growth-coupled heme...