High-cell-density cultivation of Vibrio natriegens in a low-chloride chemically defined medium.
Ammonium magnesium phosphate
Citrate
Corrosion
Fed-batch
High-cell-density cultivation
Precipitation
Specific growth rate
Vibrio natriegens
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
07
06
2023
accepted:
14
09
2023
revised:
12
09
2023
medline:
13
11
2023
pubmed:
24
9
2023
entrez:
23
9
2023
Statut:
ppublish
Résumé
Vibrio natriegens is a halophilic bacterium with the fastest generation time of non-pathogenic bacteria reported so far. It therefore has high potential as a production strain for biotechnological production processes or other applications in biotechnology. Culture media for V. natriegens typically contain high sodium chloride concentrations. The corresponding high chloride concentrations can lead to corrosion processes on metal surfaces in bioreactors. Here we report the development of a low-chloride chemically defined medium for V. natriegens. Sodium chloride was completely replaced by the sodium salts disodium hydrogen phosphate, disodium sulfate, and sodium citrate, while keeping the total concentration of sodium ions constant. The use of citrate prevents the occurrence of precipitates, especially of ammonium magnesium phosphate. With this defined medium, high-cell-density fed-batch cultivations in laboratory-scale bioreactors using exponential feeding yielded biomass concentrations of more than 60 g L
Identifiants
pubmed: 37741940
doi: 10.1007/s00253-023-12799-4
pii: 10.1007/s00253-023-12799-4
pmc: PMC10638117
doi:
Substances chimiques
Chlorides
0
Sodium Chloride
451W47IQ8X
Sodium
9NEZ333N27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7043-7054Informations de copyright
© 2023. The Author(s).
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