A mechanosensing mechanism controls plasma membrane shape homeostasis at the nanoscale.
bar proteins
cell biology
human
mechanobiology
membrane biophysics
mouse
physics of living systems
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
25 09 2023
25 09 2023
Historique:
received:
19
07
2021
accepted:
24
09
2023
medline:
23
10
2023
pubmed:
25
9
2023
entrez:
25
9
2023
Statut:
epublish
Résumé
As cells migrate and experience forces from their surroundings, they constantly undergo mechanical deformations which reshape their plasma membrane (PM). To maintain homeostasis, cells need to detect and restore such changes, not only in terms of overall PM area and tension as previously described, but also in terms of local, nanoscale topography. Here, we describe a novel phenomenon, by which cells sense and restore mechanically induced PM nanoscale deformations. We show that cell stretch and subsequent compression reshape the PM in a way that generates local membrane evaginations in the 100 nm scale. These evaginations are recognized by I-BAR proteins, which triggers a burst of actin polymerization mediated by Rac1 and Arp2/3. The actin polymerization burst subsequently re-flattens the evagination, completing the mechanochemical feedback loop. Our results demonstrate a new mechanosensing mechanism for PM shape homeostasis, with potential applicability in different physiological scenarios.
Identifiants
pubmed: 37747150
doi: 10.7554/eLife.72316
pii: 72316
pmc: PMC10569792
doi:
pii:
Substances chimiques
Actins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023, Quiroga et al.
Déclaration de conflit d'intérêts
XQ, NW, AD, AC, AM, FT, XT, RP, GS, MA, AL, PR No competing interests declared, MG Reviewing editor, eLife
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