Growth hormone insensitivity and adipose tissue: tissue morphology and transcriptome analyses in pigs and humans.
Adipose
Growth hormone
Laron Syndrome
Pig
Transcriptome
Journal
Pituitary
ISSN: 1573-7403
Titre abrégé: Pituitary
Pays: United States
ID NLM: 9814578
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
accepted:
18
09
2023
medline:
24
11
2023
pubmed:
25
9
2023
entrez:
25
9
2023
Statut:
ppublish
Résumé
Growth hormone receptor knockout (GHR-KO) pigs have recently been developed, which serve as a large animal model of Laron syndrome (LS). GHR-KO pigs, like individuals with LS, are obese but lack some comorbidities of obesity. The purpose of this study was to examine the histological and transcriptomic phenotype of adipose tissue (AT) in GHR-KO pigs and humans with LS. Intraabdominal (IA) and subcutaneous (SubQ) AT was collected from GHR-KO pigs and examined histologically for adipocyte size and collagen content. RNA was isolated and cDNA sequenced, and the results were analyzed to determine differentially expressed genes that were used for enrichment and pathway analysis in pig samples. For comparison, we also performed limited analyses on human AT collected from a single individual with and without LS. GHR-KO pigs have increased adipocyte size, while the LS AT had a trend towards an increase. Transcriptome analysis revealed 55 differentially expressed genes present in both depots of pig GHR-KO AT. Many significant terms in the enrichment analysis of the SubQ depot were associated with metabolism, while in the IA depot, IGF and longevity pathways were negatively enriched. In pathway analysis, multiple expected and novel pathways were significantly affected by genotype, i.e. KO vs. controls. When GH related gene expression was analyzed, SOCS3 and CISH showed species-specific changes. AT of GHR-KO pigs has several similarities to that of humans with LS in terms of adipocyte size and gene expression profile that help describe the depot-specific adipose phenotype of both groups.
Identifiants
pubmed: 37747600
doi: 10.1007/s11102-023-01355-y
pii: 10.1007/s11102-023-01355-y
doi:
Substances chimiques
Receptors, Somatotropin
0
Growth Hormone
9002-72-6
Insulin-Like Growth Factor I
67763-96-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
660-674Subventions
Organisme : NIH HHS
ID : AG059779
Pays : United States
Organisme : NIH HHS
ID : AG059779
Pays : United States
Organisme : NIH HHS
ID : AG059779
Pays : United States
Organisme : NIH HHS
ID : AG059779
Pays : United States
Organisme : NIH HHS
ID : AG059779
Pays : United States
Organisme : NIH HHS
ID : AG059779
Pays : United States
Organisme : NIH HHS
ID : AG059779
Pays : United States
Organisme : NIH HHS
ID : AG059779
Pays : United States
Organisme : NIH HHS
ID : AG059779
Pays : United States
Organisme : NIH HHS
ID : AG059779
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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