Isospectral intermediates in the photochemical reaction cycle of anion channelrhodopsin GtACR1.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
17 10 2023
17 10 2023
Historique:
received:
24
04
2023
revised:
15
08
2023
accepted:
15
09
2023
pmc-release:
17
10
2024
medline:
23
10
2023
pubmed:
26
9
2023
entrez:
26
9
2023
Statut:
ppublish
Résumé
The most effective tested optogenetic tools available for neuronal silencing are the light-gated anion channel proteins found in the cryptophyte alga Guillardia theta (GtACRs). Molecular mechanisms of GtACRs, including the photointermediates responsible for the open channel state, are of great interest for understanding their exceptional conductance. In this study, the photoreactions of GtACR1 and its D234N, A75E, and S97E mutants were investigated using multichannel time-resolved absorption spectroscopy. For each of the proteins, the analysis showed two early microsecond transitions between K-like and L-like forms and two late millisecond recovery steps. Spectral forms associated with potential molecular intermediates of the proteins were derived and their evolutions in time were analyzed. The results indicate the presence of isospectral intermediates in the photocycles and expand the range of potential intermediates responsible for the open channel state.
Identifiants
pubmed: 37749886
pii: S0006-3495(23)00586-6
doi: 10.1016/j.bpj.2023.09.009
pmc: PMC10598346
pii:
doi:
Substances chimiques
Channelrhodopsins
0
Anions
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4091-4103Subventions
Organisme : NEI NIH HHS
ID : R01 EY029343
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM140838
Pays : United States
Informations de copyright
Copyright © 2023. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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