Long Amplification PCR (LA-PCR) Detection of Azole Resistant Trichophyton indotineae.
Azole
Dermatophytosis
Long amplification PCR
Resistance
Trichophyton indotineae
Journal
Mycopathologia
ISSN: 1573-0832
Titre abrégé: Mycopathologia
Pays: Netherlands
ID NLM: 7505689
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
10
05
2023
accepted:
30
08
2023
medline:
1
12
2023
pubmed:
26
9
2023
entrez:
26
9
2023
Statut:
ppublish
Résumé
Trichphyton indotineae, a species newly designated in 2020 independent of T. interdigitale, comprises highly terbinafine (TRF)-resistant dermatophytosis that is epidemic in North India and spreding to worldwide. Some clinical isolates of T. indotineae have been resistance both TRF and azoles that might be caused the treatment failure. To detect the azole resistance strains, we developed a long amplification PCR (LA-PCR) detection method for the tandem repeat of the CYP51B (encoding sterol 14a-demethylase gene) in T. indotineae. Contrasting the drug susceptibility test results with the LA-PCR results confirmed a trend toward low susceptibility to azole antifungal agents in strains with amplifications of 9.5 kbp or greater (3 or more copies of CYP51B). Our results suggest that the method could be detected rapidly of low-susceptibility strains to azole antifungal agents.
Identifiants
pubmed: 37751125
doi: 10.1007/s11046-023-00793-3
pii: 10.1007/s11046-023-00793-3
doi:
Substances chimiques
Antifungal Agents
0
Azoles
0
Terbinafine
G7RIW8S0XP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1085-1088Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
Références
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