Tracheal microbiome and metabolome profiling in iatrogenic subglottic tracheal stenosis.
Metabolome
Subglottic tracheal stenosis
Tracheal microbiome
Journal
BMC pulmonary medicine
ISSN: 1471-2466
Titre abrégé: BMC Pulm Med
Pays: England
ID NLM: 100968563
Informations de publication
Date de publication:
26 Sep 2023
26 Sep 2023
Historique:
received:
30
03
2023
accepted:
13
09
2023
medline:
22
11
2023
pubmed:
27
9
2023
entrez:
26
9
2023
Statut:
epublish
Résumé
To study the role of microecology and metabolism in iatrogenic tracheal injury and cicatricial stenosis, we investigated the tracheal microbiome and metabolome in patients with tracheal stenosis after endotracheal intubation. We collected 16 protected specimen brush (PSB) and 8 broncho-alveolar lavage (BAL) samples from 8 iatrogenic subglottic tracheal stenosis patients, including 8 PSB samples from tracheal scar sites, 8 PSB samples from scar-free sites and 8 BAL samples, by lavaging the subsegmental bronchi of the right-middle lobe. Metagenomic sequencing was performed to characterize the microbiome profiling of 16 PSB and 8 BAL samples. Untargeted metabolomics was performed in 6 PSB samples (3 from tracheal scar PSB and 3 from tracheal scar-free PSB) using high-performance liquid chromatography‒mass spectrometry (LC‒MS). At the species level, the top four bacterial species were Neisseria subflava, Streptococcus oralis, Capnocytophaga gingivals, and Haemophilus aegyptius. The alpha and beta diversity among tracheal scar PSB, scar-free PSB and BAL samples were compared, and no significant differences were found. Untargeted metabolomics was performed in 6 PSB samples using LC‒MS, and only one statistically significant metabolite, carnitine, was identified. Pathway enrichment analysis of carnitine revealed significant enrichment in fatty acid oxidation. Our study found that carnitine levels in tracheal scar tissue were significantly lower than those in scar-free tissue, which might be a new target for the prevention and treatment of iatrogenic tracheal stenosis in the future.
Sections du résumé
BACKGROUND
BACKGROUND
To study the role of microecology and metabolism in iatrogenic tracheal injury and cicatricial stenosis, we investigated the tracheal microbiome and metabolome in patients with tracheal stenosis after endotracheal intubation.
METHODS
METHODS
We collected 16 protected specimen brush (PSB) and 8 broncho-alveolar lavage (BAL) samples from 8 iatrogenic subglottic tracheal stenosis patients, including 8 PSB samples from tracheal scar sites, 8 PSB samples from scar-free sites and 8 BAL samples, by lavaging the subsegmental bronchi of the right-middle lobe. Metagenomic sequencing was performed to characterize the microbiome profiling of 16 PSB and 8 BAL samples. Untargeted metabolomics was performed in 6 PSB samples (3 from tracheal scar PSB and 3 from tracheal scar-free PSB) using high-performance liquid chromatography‒mass spectrometry (LC‒MS).
RESULTS
RESULTS
At the species level, the top four bacterial species were Neisseria subflava, Streptococcus oralis, Capnocytophaga gingivals, and Haemophilus aegyptius. The alpha and beta diversity among tracheal scar PSB, scar-free PSB and BAL samples were compared, and no significant differences were found. Untargeted metabolomics was performed in 6 PSB samples using LC‒MS, and only one statistically significant metabolite, carnitine, was identified. Pathway enrichment analysis of carnitine revealed significant enrichment in fatty acid oxidation.
CONCLUSION
CONCLUSIONS
Our study found that carnitine levels in tracheal scar tissue were significantly lower than those in scar-free tissue, which might be a new target for the prevention and treatment of iatrogenic tracheal stenosis in the future.
Identifiants
pubmed: 37752498
doi: 10.1186/s12890-023-02654-7
pii: 10.1186/s12890-023-02654-7
pmc: PMC10523634
doi:
Substances chimiques
Carnitine
S7UI8SM58A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
361Subventions
Organisme : the Special and Joint Program of the Yunnan Provincial Science and Technology Department and Kunming Medical University
ID : 202201AY070001-277
Organisme : the Special and Joint Program of the Yunnan Provincial Science and Technology Department and Kunming Medical University
ID : 202201AY070001-265
Organisme : Science Research Foundation of Yunnan Provincial Education Department
ID : 2022J0019
Organisme : the National Natural Science Foundation of China
ID : No. 82160016
Organisme : Famous Doctors of High-level Talent Training Support Program of Yunnan Province
ID : No. YNWR-MY-2020-013
Informations de copyright
© 2023. The Author(s).
Références
Laryngoscope. 2016 Nov;126(11):E356-E361
pubmed: 27296163
Am J Respir Crit Care Med. 2019 May 1;199(9):1127-1138
pubmed: 30789747
Laryngoscope. 2015 May;125(5):1137-43
pubmed: 25290987
Front Pharmacol. 2021 Oct 26;12:760581
pubmed: 34764874
Life Sci. 2018 Feb 1;194:88-97
pubmed: 29241711
Laryngoscope. 2020 Dec;130(12):E773-E781
pubmed: 31904876
Metab Brain Dis. 2022 Oct;37(7):2497-2510
pubmed: 35881298
Annu Rev Physiol. 2019 Feb 10;81:403-428
pubmed: 30485759
Nat Rev Microbiol. 2023 Apr;21(4):222-235
pubmed: 36385637
Immunology. 2020 Feb;159(2):156-166
pubmed: 31631335
Chin Med J (Engl). 2010 Mar 5;123(5):621-7
pubmed: 20367992
Nat Commun. 2020 Nov 18;11(1):5886
pubmed: 33208745
Ann Otol Rhinol Laryngol. 2015 Sep;124(9):734-9
pubmed: 25910757
Curr Opin Pediatr. 2021 Dec 1;33(6):639-647
pubmed: 34412069
Nat Cell Biol. 2016 Aug;18(8):823-32
pubmed: 27428307
Laryngoscope. 2022 Jul;132(7):1356-1363
pubmed: 34319583
Otolaryngol Head Neck Surg. 2017 May;156(5):886-892
pubmed: 28349784
Zhonghua Jie He He Hu Xi Za Zhi. 2013 Sep;36(9):651-4
pubmed: 24423817
Eur J Med Res. 2021 May 25;26(1):48
pubmed: 34034813
Laryngoscope. 2017 Jan;127(1):179-185
pubmed: 27295947
Laryngoscope. 2005 Jun;115(6):1087-92
pubmed: 15933527
Front Physiol. 2022 Jan 14;12:794629
pubmed: 35095559
Eur Respir Rev. 2021 Jan 19;30(159):
pubmed: 33472959
Nat Commun. 2023 Feb 13;14(1):721
pubmed: 36781848
Ann Otol Rhinol Laryngol. 1994 Apr;103(4 Pt 1):319-23
pubmed: 8154776
Front Microbiol. 2021 Nov 05;12:761111
pubmed: 34803987
Nat Microbiol. 2016 Apr 04;1:16031
pubmed: 27572644
Int J Lab Hematol. 2021 Apr;43(2):244-249
pubmed: 33099872
Front Microbiol. 2022 Mar 24;13:855988
pubmed: 35401464
Biochem Biophys Res Commun. 2016 Jan 22;469(4):967-77
pubmed: 26718401
Front Microbiol. 2018 Feb 21;9:151
pubmed: 29515527
Vitam Horm. 2011;86:353-66
pubmed: 21419279
Ann Am Thorac Soc. 2015 Jun;12(6):821-30
pubmed: 25803243
Arch Otolaryngol Head Neck Surg. 2012 Sep;138(9):854-8
pubmed: 22986720
Otolaryngol Head Neck Surg. 2021 Jun;164(6):1257-1264
pubmed: 33290179
Nat Rev Genet. 2019 Jun;20(6):341-355
pubmed: 30918369
Front Cell Infect Microbiol. 2021 Aug 30;11:725284
pubmed: 34527604
mSphere. 2019 May 1;4(3):
pubmed: 31043518
Cells. 2022 Sep 01;11(17):
pubmed: 36078130