Development of a PNGase Rc Column for Online Deglycosylation of Complex Glycoproteins during HDX-MS.


Journal

Journal of the American Society for Mass Spectrometry
ISSN: 1879-1123
Titre abrégé: J Am Soc Mass Spectrom
Pays: United States
ID NLM: 9010412

Informations de publication

Date de publication:
01 Nov 2023
Historique:
medline: 2 11 2023
pubmed: 27 9 2023
entrez: 27 9 2023
Statut: ppublish

Résumé

Protein glycosylation is one of the most common PTMs and many cell surface receptors, extracellular proteins, and biopharmaceuticals are glycosylated. However, HDX-MS analysis of such important glycoproteins has so far been limited by difficulties in determining the HDX of the protein segments that contain glycans. We have developed a column containing immobilized PNGase Rc (from

Identifiants

pubmed: 37756257
doi: 10.1021/jasms.3c00268
pmc: PMC10623573
doi:

Substances chimiques

Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase EC 3.5.1.52
Glycoproteins 0
Polysaccharides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2556-2566

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Auteurs

Thomas Lambert (T)

Department of Pharmacy, University of Copenhagen, 2100 Copenhagen, Denmark.

Marius Gramlich (M)

NMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, Germany.

Luisa Stutzke (L)

Department of Pharmacy, University of Copenhagen, 2100 Copenhagen, Denmark.

Luke Smith (L)

Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, OX1 3QZ Oxford, England.

Dingyu Deng (D)

Department of Pharmacy, University of Copenhagen, 2100 Copenhagen, Denmark.

Philipp D Kaiser (PD)

NMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, Germany.

Ulrich Rothbauer (U)

NMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, Germany.
Pharmaceutical Biotechnology, Eberhard Karls University, 72074 Tübingen, Germany.

Justin L P Benesch (JLP)

Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, OX1 3QZ Oxford, England.

Cornelia Wagner (C)

Roche Pharma Research and Early Development, Large Molecule Research, Roche Innovation Center Munich, 82377 Penzberg, Germany.

Maximiliane Koenig (M)

Roche Pharma Research and Early Development, Large Molecule Research, Roche Innovation Center Munich, 82377 Penzberg, Germany.

Petr Pompach (P)

BioCev, Institute of Biotechnology of the CAS, 252 50 Prumyslova, Czech Republic.

Petr Novak (P)

BioCeV, Institute of Microbiology of the CAS, 142 20 Prumyslova, Czech Republic.

Anne Zeck (A)

NMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, Germany.

Kasper D Rand (KD)

Department of Pharmacy, University of Copenhagen, 2100 Copenhagen, Denmark.

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Classifications MeSH