Quantitative biochemical phenotypic heterogeneity of senescent macrophage at a single cell level by Synchrotron Radiation Fourier Transform Infrared Microspectroscopy.
Age-related diseases
Biochemical phenotype and phenotypic heterogeneity
Macrophage senescence
SR-FTIR microspectroscopy
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
28 09 2023
28 09 2023
Historique:
received:
05
04
2023
accepted:
31
08
2023
medline:
29
9
2023
pubmed:
28
9
2023
entrez:
28
9
2023
Statut:
epublish
Résumé
Macrophage senescence plays an important role in pathophysiological process of age-related diseases such as atherosclerosis, chronic obstructive pulmonary disease (COPD), pulmonary fibrosis, and lung cancer. After macrophage senescence, the biochemical phenotypes related to biological functions showed great heterogeneity. However, the biochemical phenotype and phenotypic heterogeneity of senescent macrophage has not been fully understood. Exploring the phenotype of biochemical substances in senescent macrophage will be helpful for understanding the function of senescent macrophage and finding out the potential mechanism between immune macrophage senescence and age-related diseases. In this study, we employed SR-FTIR microspectroscopy to detect the biochemical phenotype and phenotypic heterogeneity of single macrophage. The whole infrared spectra of senescent macrophages shifted, indicating biochemical substance changes within senescent macrophages. PCA and intercellular Euclidean distance statistical analysis based on specific spectra regions revealed dynamic changes of lipids and proteins during macrophage senescence. This proved that SR-FTIR microspectroscopy is an effective tool to detect the single cell biochemical phenotype transformation and phenotypic heterogeneity during macrophage senescence. It is of great significance to provide an evaluation method or clue for the study of cellular functions related to intracellular biochemical substances.
Identifiants
pubmed: 37768393
doi: 10.1007/s00604-023-05980-z
pii: 10.1007/s00604-023-05980-z
pmc: PMC10539409
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
416Subventions
Organisme : The Hunan Cancer Hospital Climb Plan
ID : Grant No. ZX2020005
Organisme : The Hunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for esophageal carcinoma
ID : Grant No.2020SK4005
Organisme : Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province
ID : Grant No. 2021SK51109
Organisme : Health Commission of Hunan Province
ID : Grant No. 20230227-1001
Informations de copyright
© 2023. The Author(s).
Références
Cell. 2019 Oct 31;179(4):813-827
pubmed: 31675495
Annu Rev Pathol. 2020 Jan 24;15:123-147
pubmed: 31530089
Anal Bioanal Chem. 2011 Mar;399(8):2771-8
pubmed: 21249341
Annu Rev Physiol. 2020 Feb 10;82:433-459
pubmed: 31730381
Anal Chim Acta. 2023 Aug 29;1271:341434
pubmed: 37328242
Int J Mol Sci. 2021 Oct 04;22(19):
pubmed: 34639083
Biotechnol Adv. 2012 Nov-Dec;30(6):1390-404
pubmed: 22401782
Nature. 2021 Feb;590(7844):122-128
pubmed: 33473210
J Clin Invest. 2021 Feb 15;131(4):
pubmed: 33586677
Cancer Cell. 2022 Jun 13;40(6):639-655.e13
pubmed: 35700707
Immunity. 2022 Aug 9;55(8):1448-1465.e6
pubmed: 35931085
Front Genet. 2019 Mar 01;10:8
pubmed: 30881372
Ageing Res Rev. 2021 Nov;71:101458
pubmed: 34500043
Nat Rev Mol Cell Biol. 2021 Feb;22(2):75-95
pubmed: 33328614
Cancer Cell. 2023 Jul 10;41(7):1242-1260.e6
pubmed: 37267953
Ageing Res Rev. 2021 May;67:101280
pubmed: 33581314
Stem Cells Dev. 2012 Jul 20;21(11):2000-11
pubmed: 22214206
Trends Cell Biol. 2018 Jun;28(6):436-453
pubmed: 29477613
Immunity. 2015 Mar 17;42(3):419-30
pubmed: 25786174
Anal Bioanal Chem. 2019 Nov;411(27):7157-7164
pubmed: 31492998
Cell. 2023 Jan 19;186(2):243-278
pubmed: 36599349
Pharmacol Rev. 2023 Jul;75(4):675-713
pubmed: 36732079
Anal Chem. 2019 Jan 2;91(1):588-621
pubmed: 30362708
Analyst. 2012 Jan 7;137(1):126-32
pubmed: 22076587
Nat Rev Immunol. 2022 Aug;22(8):484-498
pubmed: 34815556
Ageing Res Rev. 2020 Dec;64:101156
pubmed: 32949770
Biol Rev Camb Philos Soc. 2022 Oct;97(5):1844-1867
pubmed: 35569818
Biogerontology. 2021 Dec;22(6):571-587
pubmed: 34490541
Chem Commun (Camb). 2020 Nov 7;56(86):13237-13240
pubmed: 33030170
Immunity. 2022 Sep 13;55(9):1564-1580
pubmed: 36103853
Eur J Cell Biol. 2020 Aug;99(6):151108
pubmed: 32800277
Trends Cell Biol. 2023 Jan;33(1):9-17
pubmed: 35599179
Analyst. 2010 Feb;135(2):268-77
pubmed: 20098758
ACS Omega. 2020 Oct 16;5(46):29698-29705
pubmed: 33251405
Anal Chem. 2021 Jan 19;93(2):671-676
pubmed: 33290049