Ubiquitination detection techniques.
Ubiquitination
chemiluminescence
detection techniques
fluorescence
spectrophotometric
Journal
Experimental biology and medicine (Maywood, N.J.)
ISSN: 1535-3699
Titre abrégé: Exp Biol Med (Maywood)
Pays: England
ID NLM: 100973463
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
pmc-release:
03
04
2024
medline:
6
11
2023
pubmed:
3
10
2023
entrez:
3
10
2023
Statut:
ppublish
Résumé
Ubiquitination is an intricately regulated post-translational modification that involves the covalent attachment of ubiquitin to a substrate protein. The complex dynamic nature of the ubiquitination process regulates diverse cellular functions including targeting proteins for degradation, cell cycle, deoxyribonucleic acid (DNA) damage repair, and numerous cell signaling pathways. Ubiquitination also serves as a crucial mechanism in protein quality control. Dysregulation in ubiquitination could result in lethal disease conditions such as cancers and neurodegenerative diseases. Therefore, the ubiquitination cascade has become an attractive target for therapeutic interventions. Enormous efforts have been made to detect ubiquitination involving different detection techniques to better grasp the underlying molecular mechanisms of ubiquitination. This review discusses a wide range of techniques stretching from the simplest assays to real-time assays. This includes western blotting/immunoblotting, fluorescence assays, chemiluminescence assays, spectrophotometric assays, and nanopore sensing assays. This review compares these applications, and the inherent advantages and limitations.
Identifiants
pubmed: 37787047
doi: 10.1177/15353702231191186
pmc: PMC10625345
doi:
Substances chimiques
Ubiquitin
0
Proteins
0
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Review
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1333-1346Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM128595
Pays : United States
Déclaration de conflit d'intérêts
Declaration Of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Références
Curr Opin Cell Biol. 2004 Apr;16(2):119-26
pubmed: 15196553
Front Chem. 2020 Jan 10;7:915
pubmed: 31998699
J Cell Biol. 2003 Mar 3;160(5):629-33
pubmed: 12615908
Front Chem. 2019 Dec 04;7:816
pubmed: 31867306
J Biol Chem. 1982 Sep 10;257(17):10329-37
pubmed: 6286650
ACS Sens. 2017 Jan 27;2(1):31-45
pubmed: 28722447
Science. 2001 Nov 9;294(5545):1307-13
pubmed: 11588219
Int Immunopharmacol. 2003 Dec;3(13-14):1715-29
pubmed: 14636823
Eur J Biochem. 1995 Oct 1;233(1):42-7
pubmed: 7588772
Cell. 2004 Jan 23;116(2 Suppl):S29-32, 2 p following S32
pubmed: 15055578
Assay Drug Dev Technol. 2003 Feb;1(1 Pt 2):175-80
pubmed: 15090144
Curr Protoc Mol Biol. 2001 May;Appendix 3:Appendix 3A
pubmed: 18265031
J Biol Chem. 2000 Oct 13;275(41):31908-13
pubmed: 10906321
J Mol Biol. 2003 Nov 21;334(2):229-40
pubmed: 14607115
J Microbiol Methods. 2013 Jul;94(1):25-9
pubmed: 23611841
Stem Cells Int. 2020 Nov 13;2020:8835714
pubmed: 33273928
Protein Sci. 2008 Apr;17(4):777-84
pubmed: 18359863
Signal Transduct Target Ther. 2020 Feb 29;5(1):11
pubmed: 32296023
Nat Rev Drug Discov. 2011 Jan;10(1):29-46
pubmed: 21151032
Curr Opin Biotechnol. 1995 Feb;6(1):103-10
pubmed: 7534502
Nat Protoc. 2014 May;9(5):1146-59
pubmed: 24743421
Molecules. 2020 Nov 09;25(21):
pubmed: 33182242
J Biomol Screen. 2014 Feb;19(2):215-22
pubmed: 24436077
Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):151-6
pubmed: 9874787
New Phytol. 2018 Jan;217(2):613-624
pubmed: 28277608
Cytometry. 1998 Aug 15;34(4):159-79
pubmed: 9725457
J Mol Biol. 1987 Apr 5;194(3):531-44
pubmed: 3041007
Methods Mol Biol. 2018;1844:385-400
pubmed: 30242722
Curr Protoc Chem Biol. 2009 Dec 1;1(1):1-15
pubmed: 23839960
HFSP J. 2007 Sep;1(3):169-80
pubmed: 19404444
Nat Commun. 2016 Aug 24;7:12547
pubmed: 27552911
J Biomol Screen. 2004 Dec;9(8):695-703
pubmed: 15634796
Nat Rev Mol Cell Biol. 2012 Jul 23;13(8):508-23
pubmed: 22820888
Nat Protoc. 2006;1(3):1278-86
pubmed: 17406412
EMBO Rep. 2017 Mar;18(3):392-402
pubmed: 28082312
Cell. 2008 Aug 22;134(4):668-78
pubmed: 18724939
Anal Biochem. 2008 Apr 15;375(2):364-6
pubmed: 18154725
Anal Biochem. 2016 Oct 1;510:41-51
pubmed: 27325501
J Pharm Biomed Anal. 2019 Sep 5;173:40-46
pubmed: 31108422
Neurochem Res. 1994 Apr;19(4):391-8
pubmed: 8065495
FEBS J. 2008 Apr;275(7):1540-1555
pubmed: 18312418
Plant Cell. 2003 Jun;15(6):1347-59
pubmed: 12782728
Nat Commun. 2019 Apr 23;10(1):1870
pubmed: 31015455
Nat Methods. 2006 Mar;3(3):165-74
pubmed: 16489332
J Biomol Screen. 2000 Oct;5(5):319-28
pubmed: 11080690
Biopolymers. 2001-2002;61(3):159-79
pubmed: 11987179
Cell Biosci. 2016 Dec 20;6:62
pubmed: 28031783
Nat Struct Mol Biol. 2009 Dec;16(12):1328-30
pubmed: 19935683
Cell Res. 2016 Apr;26(4):399-422
pubmed: 27012465
J Biol Chem. 1990 Apr 15;265(11):6323-9
pubmed: 2156848
Int J Biochem Cell Biol. 2003 May;35(5):590-605
pubmed: 12672452
Chem Soc Rev. 2021 May 7;50(9):5668-5705
pubmed: 33735357
J Chem Biol. 2013 Apr 13;6(3):85-95
pubmed: 24432126
Nat Rev Mol Cell Biol. 2006 Jun;7(6):449-56
pubmed: 16625152
Nature. 2009 Apr 9;458(7239):732-6
pubmed: 19360080
Annu Rev Biochem. 2009;78:363-97
pubmed: 19489724
Nat Chem Biol. 2007 Nov;3(11):697-705
pubmed: 17948018
Nat Rev Mol Cell Biol. 2009 May;10(5):319-31
pubmed: 19352404
Dev Biol. 1999 Mar 15;207(2):322-33
pubmed: 10068466
Nat Rev Mol Cell Biol. 2016 Oct;17(10):626-42
pubmed: 27485899
Anal Biochem. 2011 Nov 1;418(1):102-10
pubmed: 21771579
Proc Natl Acad Sci U S A. 2022 Feb 8;119(6):
pubmed: 35101976
Methods Mol Biol. 2016;1439:77-98
pubmed: 27316989
J Proteomics. 2021 Jan 16;231:103996
pubmed: 33017648
Mol Biol Cell. 2001 Dec;12(12):3839-51
pubmed: 11739784
Biophys J. 2015 May 5;108(9):2340-9
pubmed: 25954891
Cell Death Differ. 2011 Sep;18(9):1393-402
pubmed: 21372847
Biochim Biophys Acta. 2004 Nov 29;1695(1-3):189-207
pubmed: 15571815
Cell. 2009 Mar 20;136(6):1098-109
pubmed: 19303852
Trends Biotechnol. 2008 Nov;26(11):622-30
pubmed: 18804297
Eur Biophys J. 2010 Jan;39(2):241-53
pubmed: 19693494
Nat Methods. 2004 Dec;1(3):203-8
pubmed: 15782195
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8247-52
pubmed: 12821780
Expert Opin Drug Discov. 2010 Sep;5(9):835-49
pubmed: 22823259
Methods Enzymol. 2005;399:663-82
pubmed: 16338388
J Virol. 2002 Mar;76(5):2043-53
pubmed: 11836382
PLoS One. 2013 Sep 05;8(9):e73482
pubmed: 24039955
Mol Cell. 2015 Apr 2;58(1):83-94
pubmed: 25752573
Methods Enzymol. 2005;398:20-32
pubmed: 16275316
Cell. 2017 Nov 2;171(4):918-933.e20
pubmed: 29033132
Anal Biochem. 1998 Jul 1;260(2):135-41
pubmed: 9657869
Front Mol Biosci. 2020 Feb 27;7:21
pubmed: 32175328
Trends Pharmacol Sci. 2004 Jan;25(1):35-41
pubmed: 14723977
Annu Rev Pharmacol Toxicol. 2009;49:73-96
pubmed: 18834306
ACS Nano. 2017 May 23;11(5):4387-4394
pubmed: 28353339
Curr Protoc Protein Sci. 2001 May;Chapter 3:Unit 3.3
pubmed: 18429169
Micromachines (Basel). 2019 Aug 13;10(8):
pubmed: 31412540
J Pharm Biomed Anal. 1998 Sep 1;17(6-7):941-53
pubmed: 9884186
Methods Mol Biol. 2011;680:3-28
pubmed: 21153370
Anal Chem. 2017 Jan 3;89(1):157-188
pubmed: 28105845
Cell Death Differ. 2010 Jan;17(1):4-13
pubmed: 19557013
Nat Med. 2014 Nov;20(11):1242-53
pubmed: 25375928
J Biol Chem. 1982 Mar 10;257(5):2543-8
pubmed: 6277905
BMC Biol. 2018 Aug 10;16(1):88
pubmed: 30097011
Biochem J. 2011 Jan 1;433(1):31-42
pubmed: 21158740
Chem Rev. 2018 Feb 14;118(3):889-918
pubmed: 28234446
Electrophoresis. 1999 Feb;20(2):418-28
pubmed: 10197449
Biochim Biophys Acta. 2004 Nov 29;1695(1-3):55-72
pubmed: 15571809
Methods Enzymol. 1995;246:300-34
pubmed: 7752929
Assay Drug Dev Technol. 2009 Aug;7(4):348-55
pubmed: 19656081
Mol Biol Cell. 2015 Dec 1;26(24):4325-32
pubmed: 26446837
Methods Enzymol. 2019;618:135-147
pubmed: 30850049
Open Biol. 2017 Oct;7(10):
pubmed: 29021212
Assay Drug Dev Technol. 2009 Dec;7(6):560-72
pubmed: 20105026
Methods Enzymol. 2008;450:79-106
pubmed: 19152857
J Protein Chem. 2000 Aug;19(6):489-98
pubmed: 11195973
Nat Cell Biol. 2016 May 27;18(6):579-86
pubmed: 27230526